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DOI :
10.3791/52056-v
November 6th, 2014
Chapters
0:05
Title
1:28
Media Preparation and Bone Marrow Isolation
3:05
Gradient Separation of Bone Marrow Cells and Cell Culturing
4:57
Results: Culturing Osteoclasts from Mouse Bone Marrow
5:56
Conclusion
破骨細胞は、体内の主要な骨吸収細胞である。大量に破骨細胞を単離する能力は、破骨細胞の生物学の理解における大きな進歩をもたらした。このプロトコルでは、培養し、インビトロで破骨細胞活性を定量化する、単離するための方法を記載する。
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