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DOI :
10.3791/52058-v
January 2nd, 2015
Chapters
0:05
Title
1:26
Synthesis of 10-nm Gold Nanoparticles (Gold NPs)
2:28
Coating of Gold NPs with BSA and Labeling with Rhodamine N-hydroxysuccinimidyl Ester (NHS)
4:30
Culture of HeLa Cells and Salmonella Bacteria
5:40
Infection of HeLa Cells by Salmonella and Gold-BSA-Rhodamine NPs Pulse Chase-labeling
7:04
Imaging and Image Analysis
9:18
Results: Salmonella Infection Rearranges the Endolysosomal System in HeLa Cells
10:58
Conclusion
この記事では、合成とナノ粒子(NPS)の蛍光標識するための方法を説明します。 NPは、真核細胞のエンドリソソーム系を標識するために、パルスチェイス実験に適用した。細胞内病原体サルモネラ·エンテリカの活動によって、エンド-リソソームシステムの操作は、ライブセルイメージングが続き、定量化した。
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