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DOI :
10.3791/52104-v
November 11th, 2014
Chapters
0:05
Title
2:09
Preparation of Baby Hamster Kidney (BHK) Cell Culture Medium for Stem Cell Factor Collection
3:25
EML Cell Culture and Depletion of Lineage Positive Cells
4:52
Separation of Lin–CD34+ and Lin–CD34– Cells
7:15
RNA Preparation and Library Construction for High-throughput Sequencing
9:54
Results: RNA-Seq Identifies Transcription Factors that Regulate EML Self-renewal and Differentiation
11:38
Conclusion
RNA测序和生物信息学分析被用来确定显著和差异表达的转录因子在小鼠EMLcells林-CD34 +和林-CD34-细胞亚群。这些转录因子可能在确定自我更新林的CD34 +和偏微分林CD34-细胞间的开关发挥重要作用。
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