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DOI :
10.3791/52115-v
•
7:51 min
November 14th, 2014
Chapters
0:05
Title
1:22
Preparation of Mouse GABAergic Medium Spiny Neuron Culture
4:10
Co-culture Preparation
5:18
Results: GABAA Rs Promote the Formation of Active Synaptic Contacts
7:25
Conclusion
个体发育过程中统筹抑制GABA能突触形成的分子机制知之甚少。为了研究这些方法中,我们已经开发出一种共培养模型系统,该系统采用了与稳定转染人胚胎肾293(HEK293)表达功能性GABA A受体的细胞一起培养的胚胎中型多棘GABA能神经元。
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