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DOI :
10.3791/52142-v
•
7:25 min
October 17th, 2014
Chapters
0:05
Title
1:13
Measurement of Association and Dissociation Rate Constants for mantATP
4:18
Measurement of the Dissociation Rate Constant for mantADP
5:19
Results: Analysis of the ATP Turnover Cycle of Kinesins
6:45
Conclusion
驱动蛋白的特点是核苷酸依赖的互动与微管:ATP周转连接到微管相互作用的循环周期。在这里,我们描述的协议分析个别碱基的转换中驱动蛋白的ATP周转周期使用荧光标记的核苷酸和停流荧光动力学。
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