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マクロファージ食作用およびアクチン重合の評価のためのハイスループット蛍光技術

DOI :

10.3791/52195-v

November 27th, 2014

November 27th, 2014

14,336 Views

1Department of Pharmacology, University of Minnesota, 2Department of Surgery, University of Minnesota, 33M Corporate Research Laboratory

Here we present a protocol to quantify phagocytosis of fluorescent particles by adherent macrophage cell line using a fluorometric method. This method facilitates a high throughput quantification of particle internalization as well as the resulting actin polymerization.

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