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Interna och Observation av fluorescerande Biomolekyler i levande mikroorganismer via elektroporation

DOI :

10.3791/52208-v

15:27 min

February 8th, 2015

February 8th, 2015

16,331 Views

1Clarendon Laboratory, Department of Physics, University of Oxford, 2Wellcome Trust Sanger Institute, Genome Center

Studies of biomolecules in vivo are crucial for understanding molecular function in a biological context. Here we describe a novel method allowing the internalization of fluorescent biomolecules, such as DNA or proteins, into living microorganisms. Analysis of in vivo data recorded by fluorescence microscopy is also presented and discussed.

Tags

Mikrobiologi

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