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JoVE Journal

Immunology and Infection

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2D 전기 영동에 의한 대 식세포의 프로테옴 프로파일 링

12.5K Views

07:53 min

November 4th, 2014

November 4th, 2014

12,455 Views

0:05

Title

1:40

Extraction of M1 (Pro-inflammatory) and M2 (Anti-inflammatory) Macrophage Proteins for 2D Electrophoresis

3:55

Isoelectrofocusing and Second Dimensional Electrophoresis

5:29

Image Acquisition and Bioinformatic Analysis

6:25

Results: Bioinformatic Analysis of M1 and M2 2D Protein Patterns

7:15

Conclusion

Macrophages are the key cells involved in host pathogenicity. Macrophages display phenotypic and functional diversity that can be analysed and detected by proteomic analysis. This article describes how to perform 2D electrophoresis of primary cultures of human macrophages differentiated into M1 or M2 phenotype.

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