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Development of an in vitro model system for studying the interaction of Equus caballus IgE with its high-affinity receptor FcεRI

DOI :

10.3791/52222-v

November 1st, 2014

November 1st, 2014

12,267 Views

1Biological Department, King Abdulaziz University, 2The Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, The University of Sheffield

The current study describes the development and applications of a genetically engineered assay system based on the transfection of rat basophilic leukemia cells with the equine FcεRIα gene. Transfected cells express a functional receptor where the release of mediators of the allergic response can be activated by IgE and antigen.

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In Vitro Model System

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