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The University of Sheffield

4 ARTICLES PUBLISHED IN JoVE

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Engineering

Using Neutron Spin Echo Resolved Grazing Incidence Scattering to Investigate Organic Solar Cell Materials
Andrew J. Parnell 1, Adam Hobson 2, Robert M. Dalgliesh 3, Richard A. L. Jones 1, Alan D. F. Dunbar 2
1Department of Physics and Astronomy, University of Sheffield, 2Department of Chemical and Biological Engineering, The University of Sheffield, 3ISIS Pulsed Neutron and Muon Source Science and Technology Facilities Council, Rutherford Appleton Laboratory

Progress has been made in utilizing spin echo resolved grazing incidence scattering (SERGIS) as a neutron scattering technique to probe the length-scales in irregular samples. Crystallites of [6,6]-phenyl-C61-butyric acid methyl ester have been probed using the SERGIS technique and the results confirmed by optical and atomic force microscopy.

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Immunology and Infection

Development of an in vitro model system for studying the interaction of Equus caballus IgE with its high-affinity receptor FcεRI
Sari Sabban 1, Hongtu Ye 2, Birgit Helm 2
1Biological Department, King Abdulaziz University, 2The Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, The University of Sheffield

The current study describes the development and applications of a genetically engineered assay system based on the transfection of rat basophilic leukemia cells with the equine FcεRIα gene. Transfected cells express a functional receptor where the release of mediators of the allergic response can be activated by IgE and antigen.

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Engineering

Casting Protocols for the Production of Open Cell Aluminum Foams by the Replication Technique and the Effect on Porosity
Erardo M. Elizondo Luna 1, Farzad Barari 2, Robert Woolley 2, Russell Goodall 1
1Department of Materials Science and Engineering, The University of Sheffield, 2Department of Mechanical Engineering, The University of Sheffield

Replication is one of the processing techniques used for the production of porous metal sponges. In this paper one implementation of the method for the production of open celled porous aluminum is shown in detail.

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Neuroscience

Electrophysiological Method for Recording Intracellular Voltage Responses of Drosophila Photoreceptors and Interneurons to Light Stimuli In Vivo
Mikko Juusola 1,2, An Dau 2, Lei Zheng 1, Diana Rien 1,2
1National Key Laboratory of Cognitive Neuroscience and Learning, Beijing Normal University, 2Department of Biomedical Science, The University of Sheffield

Sharp microelectrodes enable accurate electrophysiological characterization of photoreceptor and visual interneuron output in living Drosophila. Here we show how to use this method to record high-quality voltage responses of individual cells to controlled light stimulation. This method is ideal for studying neural information processing in insect compound eyes.

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