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DOI :
10.3791/52240-v
December 13th, 2014
Chapters
0:05
Title
1:22
Growth of Yeast Cells to Log Phase
2:21
Heat-induced Inhibition of mRNA Synthesis and Harvest of Yeast Cells
5:18
RNA Gel Electrophoresis and Northern Blot Transfer
7:07
Northern Hybridization
8:56
RNA Quantitation
10:03
Results: Down-regulation of an Intron Containing Pre-mRNA in NMD Mutants
11:59
Conclusion
特定的mRNA的稳态水平由合成的mRNA和衰减率决定。的全基因组的mRNA的降解率或特异mRNAs的衰变率可通过测定mRNA的半衰期进行测定。该协议的重点是mRNA降解率在酿酒酵母中的测量。
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