Research
Education
Sign In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Please note that all translations are automatically generated. Click here for the English version.
DOI :
10.3791/52240-v
December 13th, 2014
Chapters
0:05
Title
1:22
Growth of Yeast Cells to Log Phase
2:21
Heat-induced Inhibition of mRNA Synthesis and Harvest of Yeast Cells
5:18
RNA Gel Electrophoresis and Northern Blot Transfer
7:07
Northern Hybridization
8:56
RNA Quantitation
10:03
Results: Down-regulation of an Intron Containing Pre-mRNA in NMD Mutants
11:59
Conclusion
特定のmRNAの定常状態レベルは、mRNAの合成および減衰の速度によって決定される。ゲノムワイドなmRNAの分解速度または特定のmRNAの崩壊速度は、mRNAの半減期を決定することによって測定することができる。このプロトコルは、Saccharomyces cerevisiae中のmRNA減衰率の測定に焦点を当てています。
Tags
-- Views
Related Videos
Privacy
Terms of Use
Policies
Contact Us
Recommend to library
JoVE NEWSLETTERS
JoVE Journal
Methods Collections
JoVE Encyclopedia of Experiments
Archive
JoVE Core
JoVE Business
JoVE Science Education
JoVE Lab Manual
Faculty Resource Center
Authors
Overview
Publishing Process
Editorial Board
Scope and Policies
Peer Review
FAQ
Submit
Librarians
Testimonials
Subscriptions
Access
Resources
Library Advisory Board
ABOUT JoVE
Leadership
Copyright © 2024 MyJoVE Corporation. All rights reserved