Name: near infrared (nir) light increases expression of a marker of mitochondrial function in the mouse vestibular sensory epithelium
Uploaded: 2015-03-14T14:00:00
Description: Watch this Scientific Journal Video about Near Infrared NIr Light Increases Expression of a Marker of Mitochondrial Function in the Mouse Vestibular Sensory Epithelium at JoVE.com

Forfatterne ønsker at anerkende Dr. Paul Witting og Ms. Genevieve Fong for deres hjælp med mRNA udvinding og PCR samt Garnett Passe og Rodney Williams Memorial Foundation for støtte.

Etik Statement: Alle procedurer, der er skitseret nedenfor, blev godkendt af University of Sydney Animal Ethics Committee. 1. Dyr BEMÆRK: 1 og 8 - 9 måned gammel mus (C57 / BL6) blev opnået fra Animal Resources Centre (Perth, Australien). Mus blev opstaldet i Bosch Rodent Facility på University of Sydney. <ol><li> Husmus i standard mus bure på en 12/12 timers lys / mørke-cyklus med adgang til foder og vand ad libitum. </li><li> Divide mus i hver ...

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De repræsentative resultater er beskrevet her viser, at korte transkraniel levering af NIR lys (90 sek / dag i 5 dage) er tilstrækkelig til at hæve niveauet af antioxidant ekspression i ældre mus sammenlignet med sham-behandlede mus. Mens udsendte varme kunne udgøre en kilde til mitokondrie og / eller neuronal aktivering, som rapporteret for rotte vestibulære afferenter 24 - vores måling af varme, der afgives af NIR LED anordningen var &lt;0,2 ° C i løbet af 90 sek, og som sådan er usandsynligt at f...

Faldende balance ydeevne og efterfølgende fald er fælles, og desværre ofte afgørende træk af naturlige aldring 1. Virkningen af ​​dette fald kan være både fysisk og socialt, og reducerer livskvaliteten for ældre mennesker. Som reaktion herpå har fysiske behandlinger og genoptræning været genstand for forskning i fald, men har ikke været forbundet med en vedvarende reduktion af forekomsten af ​​gentagne fald. På samme tid, arbejde undersøge ændringer i det perifere eller centrale vestibulære system (systemet er ansvarlig for at opretholde balancen) er knappe, og potentielle terapeutiske strategier rettet mod disse systemer og de underliggende årsager til ubalance begrænset. Nyere arbejde på aldersbetingede associeret neurodegenerative lidelser, herunder aldersrelateret maculadegeneration 2-4, Alzheimers sygdom modeller 5-8, og Parkinsons sygdom 9-12 har vist neurobeskyttende virkninger af simple non-invasiv anvendelse af nær infrarød (NIR) lys. Endvidere i vestibulære system, NIR er blevet anvendt til at øge aktiviteten af vestibulære primære afferente neuroner in vitro 13. Mens den mekanisme af Nir lys ikke er godt forstået, har de fleste undersøgelser med anvendelse af Nir foreslået, at Nir stimulerer mitokondrier kompleks IV (cytochrom c oxidase) 14-17 for at lette cellulær metabolisme. I det vestibulære sensoriske epithel den subkutikulær plade af typen I hårceller er tæt på mitokondrier 18 og som sådan kan udgøre en virkningssted for terapeutisk NIR behandling. Her en kort, ikke-invasiv behandling regime transcranially anvendt Nir der kan anvendes til at måle cellulær metabolisme (og implicit mitokondriefunktion) i mus vestibulære sensoriske epithel er beskrevet. Også diskuteres er et præparat af den vestibulære sensoriske epithel og det godtgøres, at Nir øger udtryk for en ubiquitoos antioxidant (superoxiddismutase 1) i det sensoriske epithel - tidligere vist at være vigtigt for cochlea hår celleoverlevelse 19.

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near infrared (nir) light increases expression of a marker of mitochondrial function in the mouse vestibular sensory epithelium

Strategier for formildende fald i balance funktion med stigende alder er overvejende fokuseret på fysiske behandlinger, herunder balance opgaver og motion. Men disse tiltag ikke de underliggende årsager til balance tilbagegang. Anvendelse af mus blev virkningerne af nær infrarødt lys (NIR) på metabolismen af ​​celler i det vestibulære sensoriske epithel vurderes. Data indsamlet, viser, at denne enkle og sikker indgriben kan beskytte disse sårbare celler fra de skadelige virkninger af naturlig aldring. mRNA blev ekstraheret fra det isolerede perifere vestibulære sensoriske epithel (crista ampullaris og utriculare macula) og efterfølgende transkriberes ind i en cDNA-bibliotek. Dette bibliotek blev derefter probet for ekspressionen af ​​allestedsnærværende antioxidant (SOD-1). Antioxidant genekspression blev derefter anvendt til at kvantificere cellulær metabolisme. Brug af transkraniel levering af Nir i unge (4 uger) og ældre (8-9 måneder) mus, og en kort behandling regime (90 sek / dag i 5 days) dette arbejde foreslår Nir alene kan være tilstrækkelig til at forbedre mitokondrisk funktion i det vestibulære sensoriske epithel. Da der i øjeblikket ikke til rådighed, overkommelige, non-invasive metoder til behandling for at forbedre vestibulære hår celle funktion, anvendelse af eksterne NIR-stråling giver en potentiel strategi til at modvirke aldringens konsekvenser for cellulær metabolisme inthe vestibulære sensoriske epithel.

For at sammenligne virkningen af ​​NIR behandling i unge (4 uger) og ældre (8-9 måneder) mus målte vi ekspressionen af ​​antioxidant superoxiddismutase 1 (SOD-1) i unge (n = 16) og ældre (n = 20) mus, der var NIR-behandlede, sham-behandlede eller NIR-blokeret. Figur 2 viser en betydelig stigning i β-actin-normaliseret SOD-1-ekspression på mere end 2-fold i unge NIR-behandlede dyr sammenlignet med unge sham-behandlede dyr (p &lt;0,01) og unge NIR-blokerede dyr (p &lt;0,01). Ældre NIR-behan...

Watch this Scientific Journal Video about Near Infrared NIr Light Increases Expression of a Marker of Mitochondrial Function in the Mouse Vestibular Sensory Epithelium at JoVE.com

Near Infrared NIr Light Increases Expression of a Marker of Mitochondrial Function in the Mouse Vestibular Sensory Epithelium

Nærheden Infrarød (NIR) lys Øger Angivelse af en markør for mitokondriefunktion i Mouse vestibulære sensoriske epithel

Mitokondriel dysfunktion er kendetegnende for cellulær ældning. Dette papir anvender ikke-invasive nær-infrarøde (NIR) behandling for at forbedre mitokondrisk funktion i den aldrende mus vestibulære sensoriske epithel.

Strategies for attenuating decline in balance function with increasing age are predominantly focused on physical therapies including balance tasks and ...

Research

JoVE Journal

Biology

Near Infrared (NIr) Light Increases Expression of a Marker of Mitochondrial Function in the Mouse Vestibular Sensory Epithelium

Exploring Cognitive Functions in Babies, Children &amp; Adults with Near Infrared Spectroscopy

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In vitro Reconstitution of the Active T. castaneum Telomerase

In vitro Reconstitution of the Active T. castaneum Telomerase

Efforts to isolate the catalytic subunit of telomerase, TERT, in sufficient quantities for structural studies, have been met with limited success for more ...

Using High Resolution Computed Tomography to Visualize the Three Dimensional Structure and Function of Plant Vasculature

Brug High Resolution Computed Tomography at visualisere de tredimensionelle struktur og funktion af dyrs vaskulatur

High resolution x-ray computed tomography (HRCT) is a non-destructive diagnostic imaging technique with sub-micron resolution capability that is now being ...

MicroRNA Expression Profiles of Human iPS Cells, Retinal Pigment Epithelium Derived From iPS, and Fetal Retinal Pigment Epithelium

MicroRNA Expression Profiler af menneskelige iPS celler, retinale pigmentepitel arvet fra iPS og Føtal retinale pigmentepitel

The objective of this report is to describe the protocols for comparing the microRNA (miRNA) profiles of human induced-pluripotent stem (iPS) cells, ...

Method for the Assessment of Effects of a Range of Wavelengths and Intensities of Red/near-infrared Light Therapy on Oxidative Stress In Vitro

Method for the Assessment of Effects of a Range of Wavelengths and Intensities of Red/near-infrared Light Therapy on Oxidative Stress In Vitro

Metode til vurdering af effekter af en række bølgelængder og intensiteter af Rød / nær-infrarødt lys terapi på oxidativt stress<em&gt; In vitro</em

Red/near-infrared light therapy (R/NIR-LT), delivered by laser or light emitting diode (LED), improves functional and morphological outcomes in a range of ...

Studying Mitochondrial Structure and Function in Drosophila Ovaries

Studying Mitochondrial Structure and Function in Drosophila Ovaries

Studere Mitokondriel struktur og funktion i<em&gt; Drosophila</em&gt; ovarier

Analysis of the mitochondrial structure-function relationship is required for a thorough understanding of the regulatory mechanisms of mitochondrial ...

Primary Cell Cultures from the Mouse Retinal Pigment Epithelium

Primære cellekulturer fra mus retinale Pigment epitel

The retinal pigment epithelium (RPE) is a highly polarized multi-functional epithelium that is located between the neural retina and the choroid of the ...