Name: near infrared (nir) light increases expression of a marker of mitochondrial function in the mouse vestibular sensory epithelium
Uploaded: 2015-03-14T14:00:00
Description: Watch this Scientific Journal Video about Near Infrared NIr Light Increases Expression of a Marker of Mitochondrial Function in the Mouse Vestibular Sensory Epithelium at JoVE.com

Les auteurs tiennent à remercier le Dr Paul Witting et Mme Geneviève Fong pour leur aide dans l&#39;extraction de l&#39;ARNm et la PCR, et de la Passe Garnett et Rodney Williams Memorial Foundation pour l&#39;appui.

Déclaration éthique: Toutes les procédures décrites ci-dessous ont été approuvés par l&#39;Université du comité d&#39;éthique animale Sydney. 1. Les animaux NOTE: 1 et 8-9 mois, vieux souris (C57 / BL6) ont été obtenues auprès du Centre des ressources animales (Perth, Australie). Les souris ont été logés dans la facilité Bosch rongeurs à l&#39;Université de Sydney. <ol><li> Maison souris dans des cages standard de la souris sur un 12/12 h cyc...

<ol>
	<li>Agrawal, Y., Carey, J. P., Della Santina, ., C, C., Schubert, M. C., Minor, L. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Disorders+of+balance+and+vestibular+function+in+US+adults:+data+from+the+National+Health+and+Nutrition+Examination+Survey,+2001-2004.">Disorders of balance and vestibular function in US adults: data from the National Health and Nutrition Examination Survey, 2001-2004.</a> Archives of internal medicine. 169, 938-944 (2009).</li><li>Bessho, K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effect+of+subthreshold+infrared+laser+treatment+for+drusen+regression+on+macular+autofluorescence+in+patients+with+age-related+macular+degeneration.">Effect of subthreshold infrared laser treatment for drusen regression on macular autofluorescence in patients with age-related macular degeneration.</a> Retina. 25, 981-988 (2005).</li><li>Olk, R. J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Therapeutic+benefits+of+infrared+(810-nm)+diode+laser+macular+grid+photocoagulation+in+prophylactic+treatment+of+nonexudative+age-related+macular+degeneration:+two-year+results+of+a+randomized+pilot+study.">Therapeutic benefits of infrared (810-nm) diode laser macular grid photocoagulation in prophylactic treatment of nonexudative age-related macular degeneration: two-year results of a randomized pilot study.</a> Ophthalmology. 106, 2082-2090 (1999).</li><li>Rodanant, N., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Predictors+of+drusen+reduction+after+subthreshold+infrared+(810+nm)+diode+laser+macular+grid+photocoagulation+for+nonexudative+age-related+macular+degeneration.">Predictors of drusen reduction after subthreshold infrared (810 nm) diode laser macular grid photocoagulation for nonexudative age-related macular degeneration.</a> American journal of ophthalmology. 134, 577-585 (2002).</li><li>De Taboada, L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Transcranial+laser+therapy+attenuates+amyloid-beta+peptide+neuropathology+in+amyloid-beta+protein+precursor+transgenic+mice.">Transcranial laser therapy attenuates amyloid-beta peptide neuropathology in amyloid-beta protein precursor transgenic mice.</a> Journal of Alzheimer's disease : JAD. 23, 521-535 (2011).</li><li>Grillo, S. L., Duggett, N. A., Ennaceur, A., Chazot, P. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Non-invasive+infra-red+therapy+(1072+nm)+reduces+beta-amyloid+protein+levels+in+the+brain+of+an+Alzheimer's+disease+mouse+model.">Non-invasive infra-red therapy (1072 nm) reduces beta-amyloid protein levels in the brain of an Alzheimer's disease mouse model.</a> TASTPM. Journal of photochemistry and photobiology. B, Biology. 123, 13-22 (2013).</li><li>Purushothuman, S., Johnstone, D. M., Nandasena, C., Mitrofanis, J., Stone, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Photobiomodulation+with+near+infrared+light+mitigates+Alzheimer's+disease-related+pathology+in+cerebral+cortex+-+evidence+from+two+transgenic+mouse+models.">Photobiomodulation with near infrared light mitigates Alzheimer's disease-related pathology in cerebral cortex - evidence from two transgenic mouse models.</a> Alzheimer's researc., &amp; therapy. 6, 2 (2014).</li><li>Sommer, A. P., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=670+nm+laser+light+and+EGCG+complementarily+reduce+amyloid-beta+aggregates+in+human+neuroblastoma+cells:+basis+for+treatment+of+Alzheimer's+disease.">670 nm laser light and EGCG complementarily reduce amyloid-beta aggregates in human neuroblastoma cells: basis for treatment of Alzheimer's disease.</a> Photomedicine and laser surgery. 30, 54-60 (2012).</li><li>Moro, C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Photobiomodulation+preserves+behaviour+and+midbrain+dopaminergic+cells+from+MPTP+toxicity:+evidence+from+two+mouse+strains.">Photobiomodulation preserves behaviour and midbrain dopaminergic cells from MPTP toxicity: evidence from two mouse strains.</a> BMC neuroscience. 14, 40 (2013).</li><li>Peoples, C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Photobiomodulation+enhances+nigral+dopaminergic+cell+survival+in+a+chronic+MPTP+mouse+model+of+Parkinson's+disease.">Photobiomodulation enhances nigral dopaminergic cell survival in a chronic MPTP mouse model of Parkinson's disease.</a> Parkinsonis., &amp; related. 18, 469-476 (2012).</li><li>Shaw, V. E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Neuroprotection+of+midbrain+dopaminergic+cells+in+MPTP-treated+mice+after+near-infrared+light+treatment.">Neuroprotection of midbrain dopaminergic cells in MPTP-treated mice after near-infrared light treatment.</a> The Journal of comparative neurology. 518, 25-40 (2010).</li><li>Ying, R., Liang, H. L., Whelan, H. T., Eells, J. T., Wong-Riley, M. T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Pretreatment+with+near-infrared+light+via+light-emitting+diode+provides+added+benefit+against+rotenone-+and+MPP+-induced+neurotoxicity.">Pretreatment with near-infrared light via light-emitting diode provides added benefit against rotenone- and MPP+-induced neurotoxicity.</a> Brain research. 1243, 167-173 (2008).</li><li>Rajguru, S. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Infrared+photostimulation+of+the+crista+ampullaris.">Infrared photostimulation of the crista ampullaris.</a> The Journal of physiology. 589, 1283-1294 (2011).</li><li>Chung, H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+nuts+and+bolts+of+low-level+laser+(light)+therapy.">The nuts and bolts of low-level laser (light) therapy.</a> Annals of biomedical engineering. 40, 516-533 (2012).</li><li>Desmet, K. D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Clinical+and+experimental+applications+of+NIR-LED+photobiomodulation.">Clinical and experimental applications of NIR-LED photobiomodulation.</a> Photomedicine and laser surgery. 24, 121-128 (2006).</li><li>Huang, Y. Y., Chen, A. C., Carroll, J. D., Hamblin, M. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Biphasic+dose+response+in+low+level+light+therapy.">Biphasic dose response in low level light therapy.</a> Dose-response : a publication of International Hormesis Society. 7, 358-383 (2009).</li><li>Rojas, J. C., Gonzalez-Lima, F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Low-level+light+therapy+of+the+eye+and+brain.">Low-level light therapy of the eye and brain.</a> Eye and brain. 3, 49-67 (2011).</li><li>Vranceanu, F., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Striated+organelle,+a+cytoskeletal+structure+positioned+to+modulate+hair-cell+transduction.">Striated organelle, a cytoskeletal structure positioned to modulate hair-cell transduction.</a> Proceedings of the National Academy of Sciences of the United States of America. 109, 4473-4478 (2012).</li><li>Johnson, K. R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Separate+and+combined+effects+of+Sod1+and+Cdh23+mutations+on+age-related+hearing+loss+and+cochlear+pathology+in+C57BL/6J+mice.">Separate and combined effects of Sod1 and Cdh23 mutations on age-related hearing loss and cochlear pathology in C57BL/6J mice.</a> Hearing research. 268, 85-92 (2010).</li><li>Tung, V. W., Di Marco, S., Lim, R., Brichta, A. M., Camp, A. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=An+isolated+semi-intact+preparation+of+the+mouse+vestibular+sensory+epithelium+for+electrophysiology+and+high-resolution+two-photon+microscopy.">An isolated semi-intact preparation of the mouse vestibular sensory epithelium for electrophysiology and high-resolution two-photon microscopy.</a> Journal of visualized experiments : JoVE. , e50471 (2013).</li><li>Kirby, J., Menzies, F. M., Cookson, M. R., Bushby, K., Shaw, P. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Differential+gene+expression+in+a+cell+culture+model+of+SOD1-related+familial+motor+neurone+disease.">Differential gene expression in a cell culture model of SOD1-related familial motor neurone disease.</a> Human molecular genetics. 11, 2061-2075 (2002).</li><li>Parry, S. N., Ellis, N., Li, Z., Maitz, P., Witting, P. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Myoglobin+induces+oxidative+stress+and+decreases+endocytosis+and+monolayer+permissiveness+in+cultured+kidney+epithelial+cells+without+affecting+viability.">Myoglobin induces oxidative stress and decreases endocytosis and monolayer permissiveness in cultured kidney epithelial cells without affecting viability.</a> Kidney and Blood Pressure Research. 31, 16-28 (2008).</li><li>Saee-Rad, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Analysis+of+superoxide+dismutase+1,+dual-specificity+phosphatase+1,+and+transforming+growth+factor,+beta+1+genes+expression+in+keratoconic+and+non-keratoconic+corneas.">Analysis of superoxide dismutase 1, dual-specificity phosphatase 1, and transforming growth factor, beta 1 genes expression in keratoconic and non-keratoconic corneas.</a> Molecular vision. 19, 2501-2507 (2013).</li><li>Albert, E. S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=TRPV4+channels+mediate+the+infrared+laser-evoked+response+in+sensory+neurons.">TRPV4 channels mediate the infrared laser-evoked response in sensory neurons.</a> Journal of neurophysiology. 107, 3227-3234 (2012).</li><li>Moro, C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Photobiomodulation+inside+the+brain:+a+novel+method+of+applying+near-infrared+light+intracranially+and+its+impact+on+dopaminergic+cell+survival+in+MPTP-treated+mice.">Photobiomodulation inside the brain: a novel method of applying near-infrared light intracranially and its impact on dopaminergic cell survival in MPTP-treated mice.</a> Journal of neuroscience. 120, 670-683 (2014).</li><li>Moreno, L. E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Infrared+neural+stimulation:+beam+path+in+the+guinea+pig+cochlea.">Infrared neural stimulation: beam path in the guinea pig cochlea.</a> Hearing research. 282, 289-302 (2011).</li><li>Curthoys, I. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+red+thread+as+a+guide+in+the+vestibular+labyrinth.">A red thread as a guide in the vestibular labyrinth.</a> The Journal of physiology. 589, 1241-1241 (2011).</li><li>Chakrabarti, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mitochondrial+Dysfunction+during+Brain+Aging:+Role+of+Oxidative+Stress+and+Modulation+by+Antioxidant+Supplementation.">Mitochondrial Dysfunction during Brain Aging: Role of Oxidative Stress and Modulation by Antioxidant Supplementation.</a> Aging and disease. 2, 242-256 (2011).</li><li>Petrosillo, G., De Benedictis, V., Ruggiero, F. M., Paradies, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Decline+in+cytochrome+c+oxidase+activity+in+rat-brain+mitochondria+with+aging.+Role+of+peroxidized+cardiolipin+and+beneficial+effect+of+melatonin.">Decline in cytochrome c oxidase activity in rat-brain mitochondria with aging. Role of peroxidized cardiolipin and beneficial effect of melatonin.</a> Journal of bioenergetics and biomembranes. 45, 431-440 (2013).</li><li>Zhu, H., Sun, A., Zou, Y., Ge, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Inducible+metabolic+adaptation+promotes+mesenchymal+stem+cell+therapy+for+ischemia:+a+hypoxia-induced+and+glycogen-based+energy+prestorage+strategy.">Inducible metabolic adaptation promotes mesenchymal stem cell therapy for ischemia: a hypoxia-induced and glycogen-based energy prestorage strategy.</a> Arteriosclerosis, thrombosis, and vascular biology. 34, 870-876 (2014).</li></ol>

Les résultats représentatifs décrits ici montrent que brève prestation transcrânienne de la lumière NIR (90 sec / jour pendant 5 jours) est suffisant pour élever les niveaux d&#39;expression antioxydant chez les souris âgées par rapport aux souris sham. Alors que la chaleur émise pourrait représenter une source de mitochondrial et / ou l&#39;activation neuronale, tels que déclarés pour vestibulaire de rat afférences 24 - notre mesure de la chaleur émise par le NIR LED dispositif était &lt;0,2 ...

Baisse du rendement de l&#39;équilibre et chutes ultérieures sont communs, et les caractéristiques définissant malheureusement souvent de vieillissement naturel 1. L&#39;impact de cette baisse peut être à la fois physique et social, et réduit considérablement la qualité de vie des personnes âgées. En réponse, les thérapies physiques et de réhabilitation ont fait l&#39;objet de la recherche sur les chutes, mais ne ont pas été associés à une réduction constante de la prévalence des chutes répétées. Dans le même temps, le travail d&#39;instruction des changements dans le système vestibulaire périphérique ou central (le système chargé de maintenir l&#39;équilibre) est rare, et les stratégies thérapeutiques potentielles ciblant ces systèmes et les causes sous-jacentes du déséquilibre limitée. Des travaux récents sur les troubles neurodégénératifs liés à l&#39;âge, y compris liée à l&#39;âge, la dégénérescence maculaire 2-4 modèles de la maladie d&#39;Alzheimer 5-8, et la maladie de Parkinson 9-12 ont montré des effets neuroprotecteurs de simple application non invasive du proche infrarouge (NIR) de lumière. En outre, dans le système vestibulaire, NIR a été utilisé pour augmenter l&#39;activité des neurones afférents primaires vestibulaires 13 in vitro. Bien que le mécanisme de la lumière NIR ne est pas bien compris, la plupart des études par NIR ont suggéré que Nir stimule les mitochondries complexe IV (cytochrome c oxydase) 14-17 pour faciliter le métabolisme cellulaire. Dans l&#39;épithélium vestibulaire sensoriel la plaque sous-cutanée de cellules Je cheveux de type est dense dans les mitochondries 18 et en tant que telle peut représenter un site d&#39;action pour le traitement Nir thérapeutique. Ici, un bref régime de traitement non invasif des transcranially appliqué Nir qui peut être utilisé pour mesurer le métabolisme cellulaire (et par voie de conséquence, la fonction mitochondriale) dans l&#39;épithélium vestibulaire sensoriel de la souris est décrit. En outre examiné est une préparation de l&#39;épithélium sensoriel vestibulaire et il est montré que NIR augmente l&#39;expression d&#39;un ubiquitonous antioxydant (superoxyde dismutase 1) dans l&#39;épithélium sensoriel - précédemment révélé important pour les cheveux de la survie cellulaire 19 cochlée.

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			<td height="42" style="height:42px;width:216px;">Name of Material/ Equipment</td>
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			<td style="width:167px;">Comments/Description</td>
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			<td height="42" style="height:42px;">Quantum WARP 10</td>
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			<td style="width:119px;">2070N030-A</td>
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			<td height="63" style="height:63px;">Screw top microtubules</td>
			<td style="width:101px;">Quality Scientific Plastics</td>
			<td style="width:119px;">520-GRD-Q</td>
			<td></td>
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			<td height="63" style="height:63px;width:216px;">Ketamine</td>
			<td style="width:101px;">Parnell, Alexandria Australia</td>
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			<td></td>
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			<td height="21" style="height:21px;">Standard Pattern Scissors</td>
			<td style="width:101px;">FST</td>
			<td style="width:119px;">14001-12</td>
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			<td height="42" style="height:42px;width:216px;">Carbon steel Surgical Blades #22</td>
			<td style="width:101px;">Livingstone</td>
			<td style="width:119px;">SBLDCL 22</td>
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			<td height="21" style="height:21px;width:216px;">Friedman-Pearson Rongeurs</td>
			<td style="width:101px;">FST</td>
			<td style="width:119px;">16221-14</td>
			<td></td>
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			<td height="42" style="height:42px;width:216px;">Stereo microscope</td>
			<td style="width:101px;">Leica Microsystems</td>
			<td style="width:119px;">A60S</td>
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			<td height="21" style="height:21px;width:216px;">Dumont #5 SF Forceps</td>
			<td style="width:101px;">FST</td>
			<td style="width:119px;">11252-00</td>
			<td></td>
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			<td height="21" style="height:21px;width:216px;">Isolate II RNA Micro Kit</td>
			<td style="width:101px;">Bioline</td>
			<td style="width:119px;">BIO-52075</td>
			<td></td>
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near infrared (nir) light increases expression of a marker of mitochondrial function in the mouse vestibular sensory epithelium

Stratégies d&#39;atténuation déclin de la fonction de balance avec l&#39;âge sont principalement ciblée sur les thérapies physiques y compris les tâches de l&#39;équilibre et de l&#39;exercice. Cependant, ces approches ne traitent pas les causes sous-jacentes du déclin de l&#39;équilibre. En utilisant des souris, l&#39;impact de la lumière proche infrarouge (NIR) sur le métabolisme des cellules de l&#39;épithélium vestibulaire sensoriel a été évaluée. Les données recueillies montrent que cette intervention simple et sûr peut protéger ces cellules vulnérables contre les effets délétères du vieillissement naturel. L&#39;ARNm a été extrait à partir de l&#39;épithélium sensoriel vestibulaire périphérique isolé (crête ampullaire et macule utriculaire) et ensuite transcrite en une banque d&#39;ADNc. Cette banque a été ensuite sondée pour l&#39;expression ubiquiste d&#39;antioxydant (SOD-1). L&#39;expression du gène antioxydant a ensuite été utilisé pour quantifier le métabolisme cellulaire. Utilisation de la livraison transcrânienne de NIR chez les jeunes (4 semaines) et plus âgés (8-9 mois) des souris, et un régime de traitement brève (90 secondes / jour pendant 5 joursays), ce travail suggère Nir peut suffire à améliorer la fonction mitochondriale dans l&#39;épithélium vestibulaire sensoriel. Comme il n&#39;y a pas de méthodes disponibles, abordables et non invasives de la thérapie pour améliorer la fonction des cellules ciliées vestibulaires, l&#39;application d&#39;un rayonnement NIR externe fournit une stratégie potentielle pour contrer l&#39;impact du vieillissement sur le métabolisme cellulaire INTHE épithélium vestibulaire sensoriel.

Pour comparer l&#39;impact du traitement NIR chez les jeunes (4 semaines) et plus âgés (8-9 mois) des souris, nous avons mesuré l&#39;expression d&#39;antioxydant superoxyde dismutase 1 (SOD-1) chez les jeunes (n = 16) et plus (n = 20) souris qui étaient Nir-traitée, sham-traitée, ou NIR bloqué. Figure 2 montre une augmentation significative de la β-actine normalisée SOD-1 expression de plus de 2 fois chez les jeunes Nir-animaux traités par rapport aux jeunes animaux sham (p &lt;0,01) et les j...

Watch this Scientific Journal Video about Near Infrared NIr Light Increases Expression of a Marker of Mitochondrial Function in the Mouse Vestibular Sensory Epithelium at JoVE.com

Near Infrared NIr Light Increases Expression of a Marker of Mitochondrial Function in the Mouse Vestibular Sensory Epithelium

Proche infrarouge (NIR) Lumière augmente l&#39;expression d&#39;un marqueur de mitochondrial Fonction chez la souris vestibulaire sensoriel épithélium

Dysfonction mitochondriale est une caractéristique de la sénescence cellulaire. Ce document utilise le proche infrarouge (NIR) traitement non-invasif pour améliorer la fonction mitochondriale chez la souris épithélium vestibulaire sensoriel vieillissement.

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Near Infrared (NIr) Light Increases Expression of a Marker of Mitochondrial Function in the Mouse Vestibular Sensory Epithelium

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It is becoming increasingly apparent that electroporation is the most effective way to introduce plasmid DNA or siRNA into primary cells. The Gene Pulser ...

Hi-C: A Method to Study the Three-dimensional Architecture of Genomes.

Salut-C: une méthode d&#39;étude de l&#39;architecture tridimensionnelle des génomes.

The three-dimensional folding of chromosomes compartmentalizes the genome and and can bring distant functional elements, such as promoters and enhancers, ...

Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells

Aide d&#39;un compteur de cellules automatisé pour simplifier les études d&#39;expression génique: Knockdown siRNA de l&#39;IL-4 dans l&#39;expression des gènes dépendant cellules Namalwa

The use of siRNA mediated gene knockdown is continuing to be an important tool in studies of gene expression.  siRNA studies are being conducted not only ...

In vitro Reconstitution of the Active T. castaneum Telomerase

In vitro Reconstitution of the Active T. castaneum Telomerase

La reconstitution in vitro de l&#39;actif T. La télomérase castaneum

Efforts to isolate the catalytic subunit of telomerase, TERT, in sufficient quantities for structural studies, have been met with limited success for more ...

Using High Resolution Computed Tomography to Visualize the Three Dimensional Structure and Function of Plant Vasculature

Utilisation à haute résolution tomodensitométrie pour visualiser la structure tridimensionnelle et la fonction des vaisseaux sanguins des végétaux

High resolution x-ray computed tomography (HRCT) is a non-destructive diagnostic imaging technique with sub-micron resolution capability that is now being ...

MicroRNA Expression Profiles of Human iPS Cells, Retinal Pigment Epithelium Derived From iPS, and Fetal Retinal Pigment Epithelium

Profils MicroRNA d&#39;expression de cellules iPS humaines, l&#39;épithélium pigmentaire rétinien Derived From iPS, et fœtale épithélium pigmentaire rétinien

The objective of this report is to describe the protocols for comparing the microRNA (miRNA) profiles of human induced-pluripotent stem (iPS) cells, ...

Method for the Assessment of Effects of a Range of Wavelengths and Intensities of Red/near-infrared Light Therapy on Oxidative Stress In Vitro

Method for the Assessment of Effects of a Range of Wavelengths and Intensities of Red/near-infrared Light Therapy on Oxidative Stress In Vitro

Méthode d&#39;évaluation des effets d&#39;une gamme de longueurs d&#39;onde et des intensités de Luminothérapie Rouge / proche infrarouge sur le stress oxydatif<em&gt; In Vitro</em

Red/near-infrared light therapy (R/NIR-LT), delivered by laser or light emitting diode (LED), improves functional and morphological outcomes in a range of ...

Studying Mitochondrial Structure and Function in Drosophila Ovaries

Studying Mitochondrial Structure and Function in Drosophila Ovaries

Étudier mitochondrial Structure et fonction<em&gt; Drosophila</em&gt; ovaires

Analysis of the mitochondrial structure-function relationship is required for a thorough understanding of the regulatory mechanisms of mitochondrial ...

Primary Cell Cultures from the Mouse Retinal Pigment Epithelium

Cultures de cellules primaires de l’épithélium pigmentaire rétinien de souris

The retinal pigment epithelium (RPE) is a highly polarized multi-functional epithelium that is located between the neural retina and the choroid of the ...