Name: near infrared (nir) light increases expression of a marker of mitochondrial function in the mouse vestibular sensory epithelium
Uploaded: 2015-03-14T14:00:00
Description: Watch this Scientific Journal Video about Near Infrared NIr Light Increases Expression of a Marker of Mitochondrial Function in the Mouse Vestibular Sensory Epithelium at JoVE.com

Forfatterne ønsker å erkjenne Dr. Paul Witting og Ms Genevieve Fong for deres hjelp med mRNA ekstraksjon og PCR, og Garnett Passe og Rodney Williams Memorial Foundation for støtte.

Etikk Uttalelse: Alle prosedyrer beskrevet nedenfor ble godkjent av University of Sydney Animal Ethics Committee. 1. Dyr MERK: 1 og 8 - 9 måneder gamle mus (C57 / BL6) ble hentet fra Animal Resources Centre (Perth, Australia). Mus ble plassert i Bosch gnager Facility ved University of Sydney. <ol><li> Huset mus i standard muse bur på en 12/12 timers lys / mørke syklus med tilgang til mat og vann ad libitum. </li><li> Divide mus i hver aldersgruppe i...

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De representative resultater som er beskrevet her viser at korte transcranial levering av NIR-lys (90 sek / dag i 5 dager) er tilstrekkelig til å heve nivåene av antioksidant ekspresjon i eldre mus sammenlignet med sham-behandlede mus. Mens utstrålt varme vil kunne representere en kilde for mitokondriell og / eller neuronal aktivering, som rapportert for rotte-vestibular afferenter 24 - vår måling av varmen som avgis av NIR LED-enheten var &lt;0,2 ° C i løpet av 90 sek, og som sådan er lite sannsynlig...

Degressiv ytelse og påfølgende fall er vanlig, og dessverre ofte definerende trekk ved naturlig aldring en. Virkningen av denne nedgangen kan være både fysisk og sosialt, og reduserer livskvaliteten for eldre mennesker. Som svar har fysisk behandling og rehabilitering vært fokus for forskning på fossen, men har ikke vært assosiert med jevn reduksjon i utbredelsen av gjentatte fall. Samtidig, arbeid undersøke endringer i perifer eller sentral vestibulære systemet (systemet ansvarlig for å opprettholde balanse) er mangelvare, og potensielle terapeutiske strategier rettet mot disse systemene og de underliggende årsakene til ubalanse begrenset. Nyere arbeider på alders assosiert nevrodegenerative lidelser inkludert aldersrelatert makuladegenerasjon 2-4, Alzheimers sykdom modellene 5-8, og Parkinsons sykdom 9-12 har vist nervecellene effekter av simple non-invasiv anvendelse av nær infrarød (NIR) lys. Videre, i den vestibulære systemet, NIR har vært brukt for å øke aktiviteten av vestibulære primære afferente neuroner in vitro 13. Mens mekanismen av NIR lys ikke er godt forstått, har de fleste studier ved hjelp av NIR antydet at NIR stimulerer mitokondriene kompleks IV (cytokrom c oksidase) 14-17 til rette for cellenes stoffskifte. I det vestibulære sensorisk epitel intradermal plate av type I hårceller er tett i mitokondriene 18 og kan derfor representere et område av handlingen for terapeutisk NIR behandling. Her, en kort, ikke-invasiv behandling regime av transcranially anvendt NIR som kan brukes til å måle cellulær metabolisme (og dermed, mitokondriell funksjon) i mus vestibulære sensorisk epitel er beskrevet. Også diskutert er en forberedelse av det vestibulære sensorisk epitel og det er vist at NIR øker uttrykk for en ubiquitooss antioksidant (peroksyddismutase 1) i sensorisk epitel - tidligere vist seg å være viktig for cochlea hår celle overlevelse 19.

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near infrared (nir) light increases expression of a marker of mitochondrial function in the mouse vestibular sensory epithelium

Strategier for å dempe nedgangen i balanse funksjon med økende alder er hovedsakelig fokusert på fysisk terapi omfatter balanser oppgaver og mosjon. Men disse tilnærmingene ikke de underliggende årsakene til balanse nedgang. Ved hjelp av mus, ble virkningen av nær infrarødt lys (NIR) på metabolismen av cellene i det vestibulære sensorisk epitel vurdert. Data som samles inn, viser at denne enkle og trygge inngrep kan beskytte disse sårbare cellene fra de skadelige effektene av naturlig aldring. mRNA ble ekstrahert fra den isolerte perifer vestibular sensorisk epitel (Crista ampullaris og utricular makula) og deretter transkribert til et cDNA-bibliotek. Dette bibliotek ble så undersøkt for ekspresjon av stedsnærværende antioksydant (SOD-1). Antioksidant-genekspresjon ble så brukt til å kvantifisere cellulær metabolisme. Hjelp transcranial levering av NIR i ung (4 uker) og eldre (8-9 måneder) mus, og en kort behandling regime (90 sek / dag for fem days), antyder dette arbeidet Nir alene kan være tilstrekkelig til å forbedre mitokondriell funksjon i det vestibulære sensorisk epitel. Siden det er for øyeblikket ingen tilgjengelige, rimelige, ikke-invasive metoder for behandling for å bedre vestibulære hår celle funksjon, gir bruk av ekstern NIR stråling en potensiell strategi for å motvirke effekten av aldring på cellenes stoffskifte inthe vestibular sensorisk epitel.

Å sammenligne effekten av Nir behandling i ung (4 uker) og eldre (8-9 måneder) mus vi målt uttrykk for antioksidant superoksyddismutase 1 (SOD-1) i ung (n = 16) og eldre (n = 20) mus som var NIR-behandlede, narrebehandlede eller NIR-blokkert. Figur 2 viser en betydelig økning i β-aktin normalisert SOD-1-ekspresjon av mer enn to ganger i små NIR-behandlede dyr sammenlignet med unge sham-behandlede dyr (p &lt;0,01) og unge NIR-blokkerte dyr (p &lt;0,01). Eldre NIR-behandlede dyr viste også mer enn ...

Watch this Scientific Journal Video about Near Infrared NIr Light Increases Expression of a Marker of Mitochondrial Function in the Mouse Vestibular Sensory Epithelium at JoVE.com

Near Infrared NIr Light Increases Expression of a Marker of Mitochondrial Function in the Mouse Vestibular Sensory Epithelium

Nær infrarød (NIR) Lys Øker Uttrykk for en markør av mitokondrienes funksjon i mus Vestibulær Sensory Epitel

Mitokondriell dysfunksjon er et kjennetegn på mobil senescens. Dette papiret bruker ikke-invasiv nær-infrarødt (NIR) behandling for å bedre mitokondriefunksjonen i den aldrende mus vestibular sensorisk epitel.

Strategies for attenuating decline in balance function with increasing age are predominantly focused on physical therapies including balance tasks and ...

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Biology

Near Infrared (NIr) Light Increases Expression of a Marker of Mitochondrial Function in the Mouse Vestibular Sensory Epithelium

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Efforts to isolate the catalytic subunit of telomerase, TERT, in sufficient quantities for structural studies, have been met with limited success for more ...

Using High Resolution Computed Tomography to Visualize the Three Dimensional Structure and Function of Plant Vasculature

Ved hjelp av høyoppløselig CT for å visualisere tredimensjonale struktur og funksjon Plant vasculature

High resolution x-ray computed tomography (HRCT) is a non-destructive diagnostic imaging technique with sub-micron resolution capability that is now being ...

MicroRNA Expression Profiles of Human iPS Cells, Retinal Pigment Epithelium Derived From iPS, and Fetal Retinal Pigment Epithelium

MikroRNA uttrykk profiler av menneskelige iPS celler, retinal pigment epitelet avledet fra iPS, og Fetal Netthinne Pigment Epitel

The objective of this report is to describe the protocols for comparing the microRNA (miRNA) profiles of human induced-pluripotent stem (iPS) cells, ...

Method for the Assessment of Effects of a Range of Wavelengths and Intensities of Red/near-infrared Light Therapy on Oxidative Stress In Vitro

Method for the Assessment of Effects of a Range of Wavelengths and Intensities of Red/near-infrared Light Therapy on Oxidative Stress In Vitro

Metode for vurdering av effektene av en Range av bølgelengder og intensiteter av Red / nær-infrarødt Lysterapi på oksidativt stress<em&gt; In Vitro</em

Red/near-infrared light therapy (R/NIR-LT), delivered by laser or light emitting diode (LED), improves functional and morphological outcomes in a range of ...

Studying Mitochondrial Structure and Function in Drosophila Ovaries

Studying Mitochondrial Structure and Function in Drosophila Ovaries

Studerer mitokondrie struktur og funksjon i<em&gt; Drosophila</em&gt; Eggstokkene

Analysis of the mitochondrial structure-function relationship is required for a thorough understanding of the regulatory mechanisms of mitochondrial ...

Primary Cell Cultures from the Mouse Retinal Pigment Epithelium

Primært cellekulturer fra musen netthinnens Pigment epitel

The retinal pigment epithelium (RPE) is a highly polarized multi-functional epithelium that is located between the neural retina and the choroid of the ...