Name: near infrared (nir) light increases expression of a marker of mitochondrial function in the mouse vestibular sensory epithelium
Uploaded: 2015-03-14T14:00:00
Description: Watch this Scientific Journal Video about Near Infrared NIr Light Increases Expression of a Marker of Mitochondrial Function in the Mouse Vestibular Sensory Epithelium at JoVE.com

Os autores gostariam de agradecer Dr. Paul Witting e Ms. Genevieve Fong por sua assistência com extração de mRNA e PCR, eo Garnett Passe e Rodney Williams Memorial Foundation para o apoio.

Declaração de Ética: Todos os procedimentos descritos a seguir foram aprovados pelo Comitê de Ética da Universidade de Sydney animal. 1. Animais NOTA: 1 e 8-9 meses de idade camundongos (C57 / BL6) foram obtidos a partir do Centro de Recursos Animal (Perth, Austrália). Os ratinhos foram alojados no mecanismo Bosch Roedor na Universidade de Sydney. <ol><li> Casa ratos em gaiolas-padrão do mouse em um ciclo claro / escuro de 12/12 horas de luz com acesso a...

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Os resultados representativos descritos aqui mostram que a entrega breve transcraniana de luz NIR (90 seg / dia durante 5 dias) é suficiente para elevar os níveis de expressão de antioxidantes em ratos mais velhos quando comparados com os ratos tratados por simulação. Enquanto calor emitido poderia representar uma fonte de mitocondrial e / ou ativação neuronal, como relatado para vestibular rato aferentes 24 - nossa medição do calor emitido pelo NIR LED dispositivo foi &lt;0,2 ° C durante 90 segundo...

O declínio da performance de equilíbrio e quedas subsequentes são comuns, e, infelizmente, muitas vezes definem características de envelhecimento natural 1. O impacto desse declínio pode ser tanto física e social, e reduz significativamente a qualidade de vida das pessoas mais velhas. Em resposta, terapias físicas e reabilitação têm sido o foco da investigação sobre as quedas, mas não têm sido associadas com redução consistente na prevalência de quedas repetidas. Ao mesmo tempo, o trabalho investiga as mudanças no sistema vestibular periférico ou central (o sistema responsável por manter o equilíbrio) é escassa, e potenciais estratégias terapêuticas dirigidas a esses sistemas e as causas subjacentes de desequilíbrio limitado. Trabalhos recentes sobre doenças neurodegenerativas associadas com a idade relacionada com a idade, incluindo degeneração macular 2-4, modelos da doença de Alzheimer, 5-8 e 9-12 doença de Parkinson têm mostrado efeitos neuroprotectores de simple aplicação não invasiva do infravermelho próximo (NIR) luz. Além disso, no sistema vestibular, NIR foi usada para aumentar a actividade dos neurónios aferentes primários vestibulares in vitro 13. Embora o mecanismo de NIR luz não é bem compreendido, a maioria dos estudos que usam NIR sugeriram que estimula NIR mitocôndrias complexo IV (citocromo c oxidase) 14-17 para facilitar o metabolismo celular. No epitélio sensorial vestibular da placa subcuticular do tipo células ciliadas I é denso em mitocôndrias 18 e, como tal, pode representar um local de ação para o tratamento terapêutico NIR. Aqui, uma breve regime de tratamento, não-invasivo de transcraniana aplicado NIR que podem ser utilizadas para medir o metabolismo celular (e implicitamente, a função mitocondrial) no rato epitélio sensorial vestibular é descrito. Também é discutida a preparação de um epitélio sensorial vestibular e é mostrado que o NIR aumenta a expressão de um ubiquitonos anti-oxidante (superóxido dismutase 1) no epitélio sensorial - anteriormente mostrado ser importante para a sobrevivência de células do cabelo cóclea 19.

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			<td height="42" style="height:42px;">Quantum WARP 10</td>
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			<td height="63" style="height:63px;">Screw top microtubules</td>
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			<td height="63" style="height:63px;width:216px;">Ketamine</td>
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			<td height="21" style="height:21px;">Standard Pattern Scissors</td>
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			<td height="42" style="height:42px;width:216px;">Carbon steel Surgical Blades #22</td>
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			<td height="21" style="height:21px;width:216px;">Friedman-Pearson Rongeurs</td>
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			<td height="42" style="height:42px;width:216px;">Stereo microscope</td>
			<td style="width:101px;">Leica Microsystems</td>
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			<td height="21" style="height:21px;width:216px;">Dumont #5 SF Forceps</td>
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			<td height="21" style="height:21px;width:216px;">Isolate II RNA Micro Kit</td>
			<td style="width:101px;">Bioline</td>
			<td style="width:119px;">BIO-52075</td>
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near infrared (nir) light increases expression of a marker of mitochondrial function in the mouse vestibular sensory epithelium

Estratégias para atenuar o declínio na função equilíbrio com o aumento da idade são predominantemente focado em terapias físicas, incluindo tarefas de equilíbrio e exercícios. No entanto, estas abordagens não abordam as causas subjacentes do declínio do equilíbrio. Usando ratos, o impacto da luz de infravermelho próximo (NIR) sobre o metabolismo de células do epitélio sensitivo vestibular foi avaliada. Os dados coletados mostram que essa intervenção simples e segura pode proteger essas células vulneráveis ​​dos efeitos deletérios do envelhecimento natural. ARNm foi extraído a partir do epitélio sensitivo vestibular periférico isolado (ampulares crista, e mácula utricular) e posteriormente transcrita para uma biblioteca de ADNc. Esta biblioteca foi então sondadas para a expressão de antioxidante ubíqua (SOD-1). A expressão do gene antioxidante foi então usado para quantificar o metabolismo celular. Usando entrega transcraniana de Nir em jovens (4 semanas) e mais velhos (8-9 meses), ratos, e um regime de tratamento breve (90 seg / dia para 5 days), este trabalho sugere NIR sozinho pode ser suficiente para melhorar a função mitocondrial no epitélio sensitivo vestibular. Uma vez que existem atualmente não há métodos disponíveis, acessíveis e não-invasivos de terapia para melhorar a função das células ciliadas vestibular, a aplicação de radiação NIR externa fornece uma estratégia potencial para neutralizar o impacto do envelhecimento sobre o metabolismo celular inthe epitélio sensorial vestibular.

Para comparar o impacto do tratamento NIR em jovens (4 semanas) e mais velhos (8-9 meses), camundongos medimos a expressão de antioxidante superóxido dismutase 1 (SOD-1) em jovens (n ​​= 16) e mais velhos (n = 20) os ratos que foram tratados Nir, tratamento simulado, ou NIR-bloqueados. A Figura 2 mostra um aumento significativo na normalizada SOD-1 expressão β-actina de mais de 2 vezes em jovens tratados com NIR animais jovens em comparação com os animais tratados de forma simulada (p &lt;0,01...

Watch this Scientific Journal Video about Near Infrared NIr Light Increases Expression of a Marker of Mitochondrial Function in the Mouse Vestibular Sensory Epithelium at JoVE.com

Near Infrared NIr Light Increases Expression of a Marker of Mitochondrial Function in the Mouse Vestibular Sensory Epithelium

Infravermelho Próximo (NIR) Luz aumenta a expressão de um marcador da função mitocondrial no mouse Vestibular Sensorial Epithelium

A disfunção mitocondrial é uma característica da senescência celular. Este trabalho usa não-invasivo do infravermelho próximo (NIR) de tratamento para melhorar a função mitocondrial no rato epitélio sensorial vestibular de envelhecimento.

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Near Infrared (NIr) Light Increases Expression of a Marker of Mitochondrial Function in the Mouse Vestibular Sensory Epithelium

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Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells

Usando um contador de células automatizadas para simplificar Estudos da Expressão Gênica: Knockdown siRNA de Expressão IL-4 Gene Dependente em células NAMALWA

The use of siRNA mediated gene knockdown is continuing to be an important tool in studies of gene expression.  siRNA studies are being conducted not only ...

In vitro Reconstitution of the Active T. castaneum Telomerase

In vitro Reconstitution of the Active T. castaneum Telomerase

Na reconstituição in vitro da atividade T. Telomerase castaneum

Efforts to isolate the catalytic subunit of telomerase, TERT, in sufficient quantities for structural studies, have been met with limited success for more ...

Using High Resolution Computed Tomography to Visualize the Three Dimensional Structure and Function of Plant Vasculature

Usando tomografia de tórax para visualizar a estrutura tridimensional e Função da Usina Vasculatura

High resolution x-ray computed tomography (HRCT) is a non-destructive diagnostic imaging technique with sub-micron resolution capability that is now being ...

MicroRNA Expression Profiles of Human iPS Cells, Retinal Pigment Epithelium Derived From iPS, and Fetal Retinal Pigment Epithelium

Perfis de expressão de microRNA iPS Human Cells, Retinal Pigment Epithelium Derivado de Ips, e Fetal Retinal Pigment Epithelium

The objective of this report is to describe the protocols for comparing the microRNA (miRNA) profiles of human induced-pluripotent stem (iPS) cells, ...

Method for the Assessment of Effects of a Range of Wavelengths and Intensities of Red/near-infrared Light Therapy on Oxidative Stress In Vitro

Method for the Assessment of Effects of a Range of Wavelengths and Intensities of Red/near-infrared Light Therapy on Oxidative Stress In Vitro

Método de Avaliação de efeitos de uma gama de comprimentos de onda e intensidades de Terapia da Luz Vermelha / near-infrared sobre o estresse oxidativo<em&gt; In Vitro</em

Red/near-infrared light therapy (R/NIR-LT), delivered by laser or light emitting diode (LED), improves functional and morphological outcomes in a range of ...

Studying Mitochondrial Structure and Function in Drosophila Ovaries

Studying Mitochondrial Structure and Function in Drosophila Ovaries

Estudar mitocondrial estrutura e função em<em&gt; Drosophila</em&gt; ovários

Analysis of the mitochondrial structure-function relationship is required for a thorough understanding of the regulatory mechanisms of mitochondrial ...

Primary Cell Cultures from the Mouse Retinal Pigment Epithelium

Culturas de células primárias do Mouse epithelium Retinal do Pigment

The retinal pigment epithelium (RPE) is a highly polarized multi-functional epithelium that is located between the neural retina and the choroid of the ...