Name: near infrared (nir) light increases expression of a marker of mitochondrial function in the mouse vestibular sensory epithelium
Uploaded: 2015-03-14T14:00:00
Description: Watch this Scientific Journal Video about Near Infrared NIr Light Increases Expression of a Marker of Mitochondrial Function in the Mouse Vestibular Sensory Epithelium at JoVE.com

Yazarlar mRNA çıkarma ve PCR ile yardım için Dr. Paul Witting ve Bayan Genevieve Fong kabul etmek isteyen, ve destek için Garnett Passe ve Rodney Williams Memorial Vakfı.

Etik Beyanı: Aşağıda belirtilen tüm işlemler Sydney Hayvan Etik Kurulu Üniversitesi tarafından onaylanmıştır. 1. Hayvanlar NOT: 1 ve 8 - 9 aylık fareler (C57 / BL6) Hayvan Kaynakları Merkezi (Perth, Avustralya) elde edilmiştir. Fareler Sydney Üniversitesi&#39;nde Bosch Kemirgen Tesisinde yerleştirildiler. <ol><li> Gıda ve su ad libitum erişimi olan bir 12/12 saat ışık / karanlık döngüsünde standart fare kafesleri Ev fareleri. <...

<ol>
	<li>Agrawal, Y., Carey, J. P., Della Santina, ., C, C., Schubert, M. C., Minor, L. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Disorders+of+balance+and+vestibular+function+in+US+adults:+data+from+the+National+Health+and+Nutrition+Examination+Survey,+2001-2004.">Disorders of balance and vestibular function in US adults: data from the National Health and Nutrition Examination Survey, 2001-2004.</a> Archives of internal medicine. 169, 938-944 (2009).</li><li>Bessho, K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effect+of+subthreshold+infrared+laser+treatment+for+drusen+regression+on+macular+autofluorescence+in+patients+with+age-related+macular+degeneration.">Effect of subthreshold infrared laser treatment for drusen regression on macular autofluorescence in patients with age-related macular degeneration.</a> Retina. 25, 981-988 (2005).</li><li>Olk, R. J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Therapeutic+benefits+of+infrared+(810-nm)+diode+laser+macular+grid+photocoagulation+in+prophylactic+treatment+of+nonexudative+age-related+macular+degeneration:+two-year+results+of+a+randomized+pilot+study.">Therapeutic benefits of infrared (810-nm) diode laser macular grid photocoagulation in prophylactic treatment of nonexudative age-related macular degeneration: two-year results of a randomized pilot study.</a> Ophthalmology. 106, 2082-2090 (1999).</li><li>Rodanant, N., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Predictors+of+drusen+reduction+after+subthreshold+infrared+(810+nm)+diode+laser+macular+grid+photocoagulation+for+nonexudative+age-related+macular+degeneration.">Predictors of drusen reduction after subthreshold infrared (810 nm) diode laser macular grid photocoagulation for nonexudative age-related macular degeneration.</a> American journal of ophthalmology. 134, 577-585 (2002).</li><li>De Taboada, L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Transcranial+laser+therapy+attenuates+amyloid-beta+peptide+neuropathology+in+amyloid-beta+protein+precursor+transgenic+mice.">Transcranial laser therapy attenuates amyloid-beta peptide neuropathology in amyloid-beta protein precursor transgenic mice.</a> Journal of Alzheimer's disease : JAD. 23, 521-535 (2011).</li><li>Grillo, S. L., Duggett, N. A., Ennaceur, A., Chazot, P. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Non-invasive+infra-red+therapy+(1072+nm)+reduces+beta-amyloid+protein+levels+in+the+brain+of+an+Alzheimer's+disease+mouse+model.">Non-invasive infra-red therapy (1072 nm) reduces beta-amyloid protein levels in the brain of an Alzheimer's disease mouse model.</a> TASTPM. Journal of photochemistry and photobiology. B, Biology. 123, 13-22 (2013).</li><li>Purushothuman, S., Johnstone, D. M., Nandasena, C., Mitrofanis, J., Stone, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Photobiomodulation+with+near+infrared+light+mitigates+Alzheimer's+disease-related+pathology+in+cerebral+cortex+-+evidence+from+two+transgenic+mouse+models.">Photobiomodulation with near infrared light mitigates Alzheimer's disease-related pathology in cerebral cortex - evidence from two transgenic mouse models.</a> Alzheimer's researc., &amp; therapy. 6, 2 (2014).</li><li>Sommer, A. P., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=670+nm+laser+light+and+EGCG+complementarily+reduce+amyloid-beta+aggregates+in+human+neuroblastoma+cells:+basis+for+treatment+of+Alzheimer's+disease.">670 nm laser light and EGCG complementarily reduce amyloid-beta aggregates in human neuroblastoma cells: basis for treatment of Alzheimer's disease.</a> Photomedicine and laser surgery. 30, 54-60 (2012).</li><li>Moro, C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Photobiomodulation+preserves+behaviour+and+midbrain+dopaminergic+cells+from+MPTP+toxicity:+evidence+from+two+mouse+strains.">Photobiomodulation preserves behaviour and midbrain dopaminergic cells from MPTP toxicity: evidence from two mouse strains.</a> BMC neuroscience. 14, 40 (2013).</li><li>Peoples, C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Photobiomodulation+enhances+nigral+dopaminergic+cell+survival+in+a+chronic+MPTP+mouse+model+of+Parkinson's+disease.">Photobiomodulation enhances nigral dopaminergic cell survival in a chronic MPTP mouse model of Parkinson's disease.</a> Parkinsonis., &amp; related. 18, 469-476 (2012).</li><li>Shaw, V. E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Neuroprotection+of+midbrain+dopaminergic+cells+in+MPTP-treated+mice+after+near-infrared+light+treatment.">Neuroprotection of midbrain dopaminergic cells in MPTP-treated mice after near-infrared light treatment.</a> The Journal of comparative neurology. 518, 25-40 (2010).</li><li>Ying, R., Liang, H. L., Whelan, H. T., Eells, J. T., Wong-Riley, M. T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Pretreatment+with+near-infrared+light+via+light-emitting+diode+provides+added+benefit+against+rotenone-+and+MPP+-induced+neurotoxicity.">Pretreatment with near-infrared light via light-emitting diode provides added benefit against rotenone- and MPP+-induced neurotoxicity.</a> Brain research. 1243, 167-173 (2008).</li><li>Rajguru, S. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Infrared+photostimulation+of+the+crista+ampullaris.">Infrared photostimulation of the crista ampullaris.</a> The Journal of physiology. 589, 1283-1294 (2011).</li><li>Chung, H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+nuts+and+bolts+of+low-level+laser+(light)+therapy.">The nuts and bolts of low-level laser (light) therapy.</a> Annals of biomedical engineering. 40, 516-533 (2012).</li><li>Desmet, K. D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Clinical+and+experimental+applications+of+NIR-LED+photobiomodulation.">Clinical and experimental applications of NIR-LED photobiomodulation.</a> Photomedicine and laser surgery. 24, 121-128 (2006).</li><li>Huang, Y. Y., Chen, A. C., Carroll, J. D., Hamblin, M. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Biphasic+dose+response+in+low+level+light+therapy.">Biphasic dose response in low level light therapy.</a> Dose-response : a publication of International Hormesis Society. 7, 358-383 (2009).</li><li>Rojas, J. C., Gonzalez-Lima, F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Low-level+light+therapy+of+the+eye+and+brain.">Low-level light therapy of the eye and brain.</a> Eye and brain. 3, 49-67 (2011).</li><li>Vranceanu, F., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Striated+organelle,+a+cytoskeletal+structure+positioned+to+modulate+hair-cell+transduction.">Striated organelle, a cytoskeletal structure positioned to modulate hair-cell transduction.</a> Proceedings of the National Academy of Sciences of the United States of America. 109, 4473-4478 (2012).</li><li>Johnson, K. R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Separate+and+combined+effects+of+Sod1+and+Cdh23+mutations+on+age-related+hearing+loss+and+cochlear+pathology+in+C57BL/6J+mice.">Separate and combined effects of Sod1 and Cdh23 mutations on age-related hearing loss and cochlear pathology in C57BL/6J mice.</a> Hearing research. 268, 85-92 (2010).</li><li>Tung, V. W., Di Marco, S., Lim, R., Brichta, A. M., Camp, A. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=An+isolated+semi-intact+preparation+of+the+mouse+vestibular+sensory+epithelium+for+electrophysiology+and+high-resolution+two-photon+microscopy.">An isolated semi-intact preparation of the mouse vestibular sensory epithelium for electrophysiology and high-resolution two-photon microscopy.</a> Journal of visualized experiments : JoVE. , e50471 (2013).</li><li>Kirby, J., Menzies, F. M., Cookson, M. R., Bushby, K., Shaw, P. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Differential+gene+expression+in+a+cell+culture+model+of+SOD1-related+familial+motor+neurone+disease.">Differential gene expression in a cell culture model of SOD1-related familial motor neurone disease.</a> Human molecular genetics. 11, 2061-2075 (2002).</li><li>Parry, S. N., Ellis, N., Li, Z., Maitz, P., Witting, P. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Myoglobin+induces+oxidative+stress+and+decreases+endocytosis+and+monolayer+permissiveness+in+cultured+kidney+epithelial+cells+without+affecting+viability.">Myoglobin induces oxidative stress and decreases endocytosis and monolayer permissiveness in cultured kidney epithelial cells without affecting viability.</a> Kidney and Blood Pressure Research. 31, 16-28 (2008).</li><li>Saee-Rad, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Analysis+of+superoxide+dismutase+1,+dual-specificity+phosphatase+1,+and+transforming+growth+factor,+beta+1+genes+expression+in+keratoconic+and+non-keratoconic+corneas.">Analysis of superoxide dismutase 1, dual-specificity phosphatase 1, and transforming growth factor, beta 1 genes expression in keratoconic and non-keratoconic corneas.</a> Molecular vision. 19, 2501-2507 (2013).</li><li>Albert, E. S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=TRPV4+channels+mediate+the+infrared+laser-evoked+response+in+sensory+neurons.">TRPV4 channels mediate the infrared laser-evoked response in sensory neurons.</a> Journal of neurophysiology. 107, 3227-3234 (2012).</li><li>Moro, C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Photobiomodulation+inside+the+brain:+a+novel+method+of+applying+near-infrared+light+intracranially+and+its+impact+on+dopaminergic+cell+survival+in+MPTP-treated+mice.">Photobiomodulation inside the brain: a novel method of applying near-infrared light intracranially and its impact on dopaminergic cell survival in MPTP-treated mice.</a> Journal of neuroscience. 120, 670-683 (2014).</li><li>Moreno, L. E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Infrared+neural+stimulation:+beam+path+in+the+guinea+pig+cochlea.">Infrared neural stimulation: beam path in the guinea pig cochlea.</a> Hearing research. 282, 289-302 (2011).</li><li>Curthoys, I. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+red+thread+as+a+guide+in+the+vestibular+labyrinth.">A red thread as a guide in the vestibular labyrinth.</a> The Journal of physiology. 589, 1241-1241 (2011).</li><li>Chakrabarti, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mitochondrial+Dysfunction+during+Brain+Aging:+Role+of+Oxidative+Stress+and+Modulation+by+Antioxidant+Supplementation.">Mitochondrial Dysfunction during Brain Aging: Role of Oxidative Stress and Modulation by Antioxidant Supplementation.</a> Aging and disease. 2, 242-256 (2011).</li><li>Petrosillo, G., De Benedictis, V., Ruggiero, F. M., Paradies, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Decline+in+cytochrome+c+oxidase+activity+in+rat-brain+mitochondria+with+aging.+Role+of+peroxidized+cardiolipin+and+beneficial+effect+of+melatonin.">Decline in cytochrome c oxidase activity in rat-brain mitochondria with aging. Role of peroxidized cardiolipin and beneficial effect of melatonin.</a> Journal of bioenergetics and biomembranes. 45, 431-440 (2013).</li><li>Zhu, H., Sun, A., Zou, Y., Ge, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Inducible+metabolic+adaptation+promotes+mesenchymal+stem+cell+therapy+for+ischemia:+a+hypoxia-induced+and+glycogen-based+energy+prestorage+strategy.">Inducible metabolic adaptation promotes mesenchymal stem cell therapy for ischemia: a hypoxia-induced and glycogen-based energy prestorage strategy.</a> Arteriosclerosis, thrombosis, and vascular biology. 34, 870-876 (2014).</li></ol>

Burada açıklanan temsili sonuçlar NIR ışık kısa transkraniyal dağıtım (5 gün boyunca 90 sn / gün) plasebo ile tedavi edilen fareler ile karşılaştırıldığında, daha yaşlı farelerin antioksidan ifade seviyelerini yükseltmek için yeterli olduğunu göstermektedir. Nir yaydığı ısı bizim ölçüm cihazı &lt;0,2 ° C 90 sn bitmişti LED ve gibi neden düşüktür - yayılan ısı mitokondriyal ve / veya nöronal aktivasyon kaynağı temsil edebilir ederken, sıçan vestibüler için bildirilen <su...

Denge performansını Azalan ve sonraki düşüşler yaygındır ve doğal yaşlanma 1 maalesef sıklıkla tanımlayan özellikler. Bu düşüşün etkisi fiziksel hem de sosyal olmak, ve önemli ölçüde yaşlı insanlar için yaşam kalitesini düşürür yapabilirsiniz. Buna karşılık, fizik tedaviler ve rehabilitasyon düşme içine araştırma odağı olmuştur ama tekrarlanan düşme yaygınlığı tutarlı azalma ile ilişkili değil. Aynı zamanda, iş periferik veya santral vestibüler sistemi (dengesini korumak için sorumlu sistem) kıt olduğu ve bu sistemleri hedefleyen potansiyel tedavi stratejileri değişiklik ve sınırlı dengesizliğin altında yatan nedenleri araştırmak. Yaşla ilgili maküler dejenerasyon, 2-4, Alzheimer hastalığı modelleri 5-8, ve Parkinson hastalığı dahil olmak üzere, 9-12 yaş ile ilişkili nörodejeneratif hastalıkların üzerine yapılan son çalışmalar si nöro-koruyucu etkiler göstermiştiryakın kızılötesi (NIR) ışık mple non-invaziv bir uygulama. Bundan başka, denge sisteminin, NIR in vitro 13 vestibüler birincil aferent nöronların etkinliğini geliştirmek için kullanılmıştır. Nir ışık mekanizması iyi anlaşılamamakla birlikte, Nir kullanarak çalışmaların çoğu Nir hücresel metabolizmayı kolaylaştırmak için mitokondri karmaşık IV (sitokrom-c oksidaz) 14-17 uyardığını ileri sürmüşlerdir. Vestibüler duyu epitel tip I saç hücrelerinin subkutiküler plaka mitokondri 18 yoğun olduğu ve gibi terapötik Nir tedavisi için bir eylem siteyi temsil edebilir. Burada, transcranially kısa bir invazif olmayan bir tedavi rejimi hücresel metabolizmayı ölçmek için kullanılabilir (ve dolaylı mitokondriyal fonksiyonu olarak), fare vestibüler duyusal epitel anlatılan NIR uygulanır. Ayrıca ele alınan vestibüler duyusal epitel bir preparattır ve NIR bir ubiquito ekspresyonunu arttırdığı gösterilmiştirBize Anti-oksidanın, duyusal epitel (süperoksit dismutaz 1) - daha önce koklea saç hücre hayatta kalma 19 için önemli olduğu gösterilmiştir.

<table border="0" cellpadding="0" cellspacing="0" width="603">
	<tbody>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">Name of Material/ Equipment</td>
			<td style="width:101px;">Company</td>
			<td style="width:119px;">Catalog Number</td>
			<td style="width:167px;">Comments/Description</td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;">Quantum WARP 10</td>
			<td style="width:101px;">Quantum Devices</td>
			<td style="width:119px;">2070N030-A</td>
			<td></td>
		</tr>
		<tr height="63">
			<td height="63" style="height:63px;">Screw top microtubules</td>
			<td style="width:101px;">Quality Scientific Plastics</td>
			<td style="width:119px;">520-GRD-Q</td>
			<td></td>
		</tr>
		<tr height="63">
			<td height="63" style="height:63px;width:216px;">Ketamine</td>
			<td style="width:101px;">Parnell, Alexandria Australia</td>
			<td style="width:119px;"></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Standard Pattern Scissors</td>
			<td style="width:101px;">FST</td>
			<td style="width:119px;">14001-12</td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">Carbon steel Surgical Blades #22</td>
			<td style="width:101px;">Livingstone</td>
			<td style="width:119px;">SBLDCL 22</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Friedman-Pearson Rongeurs</td>
			<td style="width:101px;">FST</td>
			<td style="width:119px;">16221-14</td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">Stereo microscope</td>
			<td style="width:101px;">Leica Microsystems</td>
			<td style="width:119px;">A60S</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Dumont #5 SF Forceps</td>
			<td style="width:101px;">FST</td>
			<td style="width:119px;">11252-00</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Isolate II RNA Micro Kit</td>
			<td style="width:101px;">Bioline</td>
			<td style="width:119px;">BIO-52075</td>
			<td></td>
		</tr>
	</tbody>
</table>

near infrared (nir) light increases expression of a marker of mitochondrial function in the mouse vestibular sensory epithelium

Artan yaş ile denge fonksiyonu düşüş zayıflatmaya yönelik stratejiler denge görevleri ve egzersiz gibi fiziksel tedaviler ağırlıklı odaklanmış bulunmaktadır. Ancak, bu yaklaşım denge düşüş yatan nedenleri ele yoktur. Fareler kullanarak, vestibüler duyu epitel hücrelerin metabolizması üzerine yakın kızılötesi ışığın etkisi (NUR) değerlendirildi. Toplanan veriler bu basit ve güvenli bir müdahale doğal yaşlanma zararlı etkilerinden bu hassas hücreleri koruyan olabileceğini göstermektedir. mRNA izole vestibüler duyusal epiteli (krista ampullaris ve utricular makula) elde edilir ve daha sonra, bir cDNA kitaplığında transkribe edilmiştir. Bu kütüphane, daha sonra her yerde antioksidan (SOD-1) ifadesi için problanmıştır. Antioksidan gen ifadesi hücre metabolizmasını ölçmek için kullanıldı. Genç Nir ve transkranial teslim (4 hafta) ve üzeri kullanma - 5 d (8 9 ay) fareler ve kısa bir tedavi rejimi (90 sn / günays), bu çalışma vestibüler duyusal epitel mitokondriyal fonksiyonu geliştirmek için yeterli olabilir ve yalnız NIR göstermektedir. Vestibüler saç hücre fonksiyonunu artırmak için tedavinin hiçbir mevcut, uygun fiyatlı, non-invaziv yöntemler şu anda olduğundan, dış NIR radyasyon uygulaması vestibüler duyu epiteli inthe hücresel metabolizma üzerinde yaşlanma etkilerini ortadan kaldırmak için potansiyel bir strateji sağlar.

Genç NIR tedavi etkisini (4 hafta) ve daha büyük karşılaştırılması - fareler (8 9 ay) (n = 20) (n = 16), genç antioksidan süperoksit dismutaz 1 (SOD-1) ifadesini ölçülür ve eski plasebo ile tedavi edilmiş, NIR-muamele ya da NIR-bloke fareler. Şekil 2, genç plasebo ile tedavi edilen hayvanlara kıyasla NIR tedavi genç hayvanlarda en fazla 2 misli β-aktin normalize SOD-1 ekspresyonunda önemli bir artış olduğunu göstermektedir (p &lt;0.01) ve genç Nir-bloke hayvanlar (p &lt;0.01)....

Watch this Scientific Journal Video about Near Infrared NIr Light Increases Expression of a Marker of Mitochondrial Function in the Mouse Vestibular Sensory Epithelium at JoVE.com

Near Infrared NIr Light Increases Expression of a Marker of Mitochondrial Function in the Mouse Vestibular Sensory Epithelium

Yakın Kızılötesi (NIR) Işık Fare Vestibüler Duyu Epitelinin Mitokondriyal Fonksiyonun bir Marker Anlatım artırır

Mitokondriyal disfonksiyon hücresel yaşlanma bir özelliğidir. Bu kağıt yaşlanma fare vestibüler duyu epitel mitokondriyal fonksiyon geliştirmek için non-invaziv yakın kızılötesi (NIR) tedavi kullanır.

Strategies for attenuating decline in balance function with increasing age are predominantly focused on physical therapies including balance tasks and ...

Research

JoVE Journal

Biology

Near Infrared (NIr) Light Increases Expression of a Marker of Mitochondrial Function in the Mouse Vestibular Sensory Epithelium

Exploring Cognitive Functions in Babies, Children &amp; Adults with Near Infrared Spectroscopy

Exploring Cognitive Functions in Babies, Children & Adults with Near Infrared Spectroscopy

Yakın Kızılötesi Spektroskopisi ile bebekler, Çocuk ve Yetişkin Bilişsel Fonksiyonları keşfetmek

An explosion of functional Near Infrared Spectroscopy (fNIRS) studies investigating cortical activation in relation to higher cognitive processes, such as ...

Using the Gene Pulser MXcell Electroporation System to Transfect Primary Cells with High Efficiency

Yüksek Verimli Primer Hücre Transfect Gen Verici MXcell Elektroporasyon Sistemi

It is becoming increasingly apparent that electroporation is the most effective way to introduce plasmid DNA or siRNA into primary cells. The Gene Pulser ...

Hi-C: A Method to Study the Three-dimensional Architecture of Genomes.

Hi-C: genomların Üç boyutlu Mimari Eğitim Bir Yöntem.

The three-dimensional folding of chromosomes compartmentalizes the genome and and can bring distant functional elements, such as promoters and enhancers, ...

Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells

Gen İfadesi Çalışmaları basitleştirin Otomatik Hücre Sayıcı kullanma: Namalwa Hücreler IL-4 Bağımlı Gen İfadesi siRNA demonte

The use of siRNA mediated gene knockdown is continuing to be an important tool in studies of gene expression.  siRNA studies are being conducted not only ...

In vitro Reconstitution of the Active T. castaneum Telomerase

In vitro Reconstitution of the Active T. castaneum Telomerase

Active T. in vitro Sulandırma castaneum Telomeraz

Efforts to isolate the catalytic subunit of telomerase, TERT, in sufficient quantities for structural studies, have been met with limited success for more ...

Using High Resolution Computed Tomography to Visualize the Three Dimensional Structure and Function of Plant Vasculature

Bitki damarları ve Üç Boyutlu Yapı ve Fonksiyon Visualize&#39;a Yüksek Çözünürlüklü Bilgisayarlı Tomografi Kullanımı

High resolution x-ray computed tomography (HRCT) is a non-destructive diagnostic imaging technique with sub-micron resolution capability that is now being ...

MicroRNA Expression Profiles of Human iPS Cells, Retinal Pigment Epithelium Derived From iPS, and Fetal Retinal Pigment Epithelium

İnsan iPS Hücreleri MicroRNA İfade Profilleri, iPS Kaynaklanan Retina pigment epiteli ve Fetal Retina pigment epiteli

The objective of this report is to describe the protocols for comparing the microRNA (miRNA) profiles of human induced-pluripotent stem (iPS) cells, ...

Method for the Assessment of Effects of a Range of Wavelengths and Intensities of Red/near-infrared Light Therapy on Oxidative Stress In Vitro

Method for the Assessment of Effects of a Range of Wavelengths and Intensities of Red/near-infrared Light Therapy on Oxidative Stress In Vitro

Dalga boyundaki ve Oksidatif Stres Kırmızı / yakın-kızılötesi ışık Terapisi şiddetler bir Range Etkilerinin Değerlendirilmesi için Yöntemi<em&gt; İn Vitro</em

Red/near-infrared light therapy (R/NIR-LT), delivered by laser or light emitting diode (LED), improves functional and morphological outcomes in a range of ...

Studying Mitochondrial Structure and Function in Drosophila Ovaries

Studying Mitochondrial Structure and Function in Drosophila Ovaries

Mitokondriyal Yapısı ve İşlevi incelenmesi<em&gt; Drosophila</em&gt; Yumurtalıklar

Analysis of the mitochondrial structure-function relationship is required for a thorough understanding of the regulatory mechanisms of mitochondrial ...

Primary Cell Cultures from the Mouse Retinal Pigment Epithelium

Fare retina Pigment epiteli birincil hücre kültürleri

The retinal pigment epithelium (RPE) is a highly polarized multi-functional epithelium that is located between the neural retina and the choroid of the ...