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DOI :
10.3791/52266-v
January 28th, 2015
Chapters
0:05
Title
1:27
Generating a Photoconvertible Fusion Construct and Injecting Dechorionated Zebrafish Embryos
3:22
Mounting Zebrafish Embryos for Photoconversion and Confocal Imaging
4:37
Photoconverting and Measuring the Decrease of the Photoconverted Signal
6:47
Analyzing the Data Using PyFDAP
8:18
Results: Half-life of Squint-Dendra2 as Determined by Fluorescence Decay After Photoconversion during Embryogenesis
9:19
Conclusion
세포와 조직에서 단백질 수준은 종종 단단히 단백질 생산 및 통관의 균형에 의해 조절된다. 광 (FDAP) 후 형광 감쇄를 사용하면, 단백질의 클리어런스 동력학 실험적 생체 내에서 측정 할 수있다.
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