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DOI :
10.3791/52281-v
January 22nd, 2015
Chapters
0:05
Title
1:44
Giant Unilamellar Vesicle Growth by Electroformation
4:29
Giant Unilamellar Vesicle Growth by Gel-assisted Swelling
6:07
Harvesting and Transferring GUVs
7:33
Patch-clamping GUVs
9:14
Results: Microscopy and Patch Clamp Studies of KvAP GUVs
11:03
Conclusion
電鋳し、ゲル支援腫れ - 巨人層小胞(GUVs)に貫通タンパク質、KvAPの再構成は、2脱水 - 再水和法について実証されている。両方の方法では、タンパク質を含む小さな単層小胞を蛍光顕微鏡法およびパッチクランプ電気生理学によって研究することができるGUVsを形成するために一緒に融合される。
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