Name: mouse pneumonectomy model of compensatory lung growth
Uploaded: 2014-12-17T14:00:00
Description: Watch this Scientific Journal Video about Mouse Pneumonectomy Model of Compensatory Lung Growth at JoVE.com

The authors would like to acknowledge the Cincinnati Children&#8217;s Hospital Division of Veterinary Services for their assistance. This manuscript was supported by the National Institutes of Health K12 HD028827. Anna Perl PhD taught the authors this surgical procedure.

Uso affermazione Animal:: NOTA Tutte le procedure di questo studio sono stati condotti con l&#39;approvazione e seguendo le linee guida del Istituzionale uso degli animali e del Comitato Care (IACUC) dell&#39;Ospedale dei bambini di Cincinnati. Otto settimane di età C57BL / 6J maschi sono stati ottenuti da Jackson Laboratories (Bar Harbor, ME) e permesso di ambientarsi per una settimana prima dell&#39;uso. Fino chirurgia, gli animali sono stati alloggiati in una struttura senza barriere-patogeno e fornito chow autoclav...

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Abbiamo fornito la descrizione più dettagliata delle procedure PNX del mouse PNX e mouse farsa segnalati fino ad oggi. Abbiamo fatto il lettore a conoscenza di alcuni dei problemi comuni che gli investigatori di apprendimento la procedura comunemente incontrano, e abbiamo delineato diverse tecniche sviluppate dal nostro laboratorio per mitigare queste trappole. Altri laboratori che utilizzano questo modello possono sviluppare altre modifiche tecnica o utilizzare strumenti diversi. Nel valutare le differenze nelle tecni...

La funzione principale del polmone è di fornire ossigeno e anidride carbonica scambio tra un organismo e l&#39;atmosfera. Negli esseri umani, una serie di condizioni congenite ed acquisite portano a ridurre la superficie del polmone che si traduce in deficit della funzionalità respiratoria. Anche se una serie di terapie come corticosteroidi inalatori, broncodilatatori, ossigeno supplementare, e la ventilazione meccanica cronica sono utilizzati per attenuare le conseguenze di funzionalità polmonare ridotta 1-3, la terapia ideale per queste condizioni dovrebbe promuovere la ricrescita del tessuto polmonare funzionale - cioè, polmone rigenerazione. Rigenerazione dei tessuti dei mammiferi è stata ben documentata. L&#39;africano Mouse spinosi può rigenerare ampie aree di pelle senza formazione di cicatrici 4. La falange distale nell&#39;uomo può rigenerarsi dopo una lesione o amputazione 5-7. Dopo pneumonectomia (PNX), la crescita del polmone di compensazione avviene nei topi 8, ratti 9, dogs 10, e gli esseri umani 11. Per definizione, la crescita del polmone di compensazione coinvolge non solo l&#39;espansione di spazi aerei esistenti, ma ri-septation di questi spazi aerei allargata con l&#39;espansione del microcircolo associato 12. Analisi di espressione genica ha dimostrato che questo modello ricapitola molti degli eventi di segnalazione dello sviluppo polmonare 13. Quattro settimane dopo il mouse PNX, superficie alveolare è equivalente a quella di fittizie animali operati 14. In questo manoscritto, descriviamo la PNX mouse e procedure PNX fittizi.

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			<td height="101" style="height:101px;width:263px;">6-inch Vascular Clip Applicator</td>
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			<td height="101" style="height:101px;width:263px;">Horizon Small Titanium Red Clip</td>
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			<td style="width:263px;">1201</td>
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			<td height="121" style="height:121px;width:263px;">Narrow Pattern 12cm Curved Forceps</td>
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			<td height="141" style="height:141px;width:263px;">Curved Serated 10 cm Graefe Forceps&#160;</td>
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			<td style="width:263px;">11052-10</td>
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			<td height="21" style="height:21px;width:263px;">Castroviejo Needle Holder</td>
			<td style="width:263px;">Fine Science Tools</td>
			<td style="width:263px;">12565-14</td>
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		<tr height="41">
			<td height="41" style="height:41px;width:263px;">Straight 9 cm Strabismus Scissors (Blunt Tip)</td>
			<td style="width:263px;">Fine Science Tools</td>
			<td style="width:263px;">14075-09</td>
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			<td height="41" style="height:41px;width:263px;">Straight 8.5 cm Hardened Fine Scissors</td>
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			<td style="width:263px;">14090-09</td>
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			<td height="41" style="height:41px;width:263px;">Straight, Blunt Tip Cohan-Vannas Spring Scissors</td>
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			<td height="21" style="height:21px;width:263px;">22 ga angiocatheter</td>
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			<td style="width:263px;"></td>
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			<td height="21" style="height:21px;width:263px;">3 mL luer lock syringe</td>
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			<td style="width:263px;"></td>
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			<td height="21" style="height:21px;width:263px;">4 short retractors</td>
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			<td height="21" style="height:21px;width:263px;">2 long retractors</td>
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			<td height="41" style="height:41px;width:263px;">5-0 Prolene on curved cutting needle</td>
			<td style="width:263px;">Ethicon</td>
			<td style="width:263px;">8698G</td>
			<td style="width:263px;"></td>
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			<td height="21" style="height:21px;width:263px;">0.5 mL syringe on 27 ga needle</td>
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			<td height="21" style="height:21px;width:263px;">Normal Saline</td>
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			<td height="21" style="height:21px;width:263px;">Buprenorphine</td>
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			<td style="width:263px;"></td>
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			<td height="21" style="height:21px;width:263px;">Press-n-Seal Wrap</td>
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			<td height="21" style="height:21px;width:263px;">12 inch X 12 inch cork board stack</td>
			<td style="width:263px;">Office Depot</td>
			<td style="width:263px;"></td>
			<td style="width:263px;"></td>
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			<td height="21" style="height:21px;width:263px;">70% Ethanol</td>
			<td style="width:263px;"></td>
			<td style="width:263px;"></td>
			<td style="width:263px;"></td>
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		<tr height="21">
			<td height="21" style="height:21px;width:263px;">Betadine</td>
			<td style="width:263px;"></td>
			<td style="width:263px;"></td>
			<td style="width:263px;"></td>
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		<tr height="21">
			<td height="21" style="height:21px;width:263px;">Mouse Ventilator</td>
			<td style="width:263px;">Hugo Sachs Elektronnik&#160;</td>
			<td style="width:263px;">Minivent Type 845</td>
			<td style="width:263px;"></td>
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			<td height="21" style="height:21px;width:263px;">Isoflurane Vaporizer</td>
			<td style="width:263px;">OHMEDA</td>
			<td style="width:263px;">Excel 210 SE</td>
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			<td height="21" style="height:21px;width:263px;">artificial tear ointment</td>
			<td style="width:263px;">puralube</td>
			<td style="width:263px;">NDC: 17033-211-38</td>
			<td style="width:263px;"></td>
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mouse pneumonectomy model of compensatory lung growth

In humans, disrupted repair and remodeling of injured lung contributes to a host of acute and chronic lung disorders which may ultimately lead to disability or death. Injury-based animal models of lung repair and regeneration are limited by injury-specific responses making it difficult to differentiate changes related to the injury response and injury resolution from changes related to lung repair and lung regeneration. However, use of animal models to identify these repair and regeneration signaling pathways is critical to the development of new therapies aimed at improving pulmonary function following lung injury. The mouse pneumonectomy model utilizes compensatory lung growth to isolate those repair and regeneration signals in order to more clearly define mechanisms of alveolar re-septation. Here, we describe our technique for performing mouse pneumonectomy and sham pneumonectomy. This technique may be utilized in conjunction with lineage tracing or other transgenic mouse models to define molecular and cellular mechanism of lung repair and regeneration.

. Una trama di PNX e sham gestito pesi del mouse viene fornito in figura 6 Nelle nostre mani, la sopravvivenza è costantemente 95-100% per entrambi PNX e sham pneumonectomia. Per una descrizione di come il polmone destro re-cresce in questo modello e il decorso previsto, rimandiamo il lettore di manoscritti di Gibney et al. 15 e Wang et al. 14 Diversi problemi comuni devono essere evitati per eseguire con successo le procedure sham pn...

Watch this Scientific Journal Video about Mouse Pneumonectomy Model of Compensatory Lung Growth at JoVE.com

Mouse Pneumonectomy Model of Compensatory Lung Growth

Topo Pneumonectomia Modello di crescita compensatoria Lung

Mouse pneumonectomy is a commonly employed model of compensatory lung growth. This procedure can be used in conjunction with lineage tracing or transgenic mouse models to elucidate underlying mechanisms.

In humans, disrupted repair and remodeling of injured lung contributes to a host of acute and chronic lung disorders which may ultimately lead to ...

Research

JoVE Journal

Medicine

A Simple Method of Mouse Lung Intubation

Un metodo semplice di intubazione mouse polmone

A  simple procedure to intubate mice for pulmonary function measurements would  have several advantages in longitudinal studies with limited numbers or  ...

Ricerca

Medicina

Diagnostic Ultrasound Imaging of Mouse Diaphragm Function

Diagnostic Ultrasound Imaging di funzione mouse Diaframma

Function analysis of rodent respiratory skeletal muscles, particularly the diaphragm, is commonly performed by isolating muscle strips using invasive ...

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Un modello murino di ischemia miocardica e riperfusione lesioni da legatura della arteria discendente anteriore sinistra

Acute or chronic myocardial infarction (MI) are cardiovascular events resulting in high morbidity and mortality. Establishing the pathological mechanisms ...

Implantation of Fibrin Gel on Mouse Lung to Study Lung-specific Angiogenesis

L&#39;impianto di fibrina gel sul mouse polmone per studiare angiogenesi Lung-specifica

Recent significant advances in stem cell research and bioengineering techniques have made great progress in utilizing biomaterials to regenerate and ...

Method of Isolated Ex Vivo Lung Perfusion in a Rat Model: Lessons Learned from Developing a Rat EVLP Program

Method of Isolated Ex Vivo Lung Perfusion in a Rat Model: Lessons Learned from Developing a Rat EVLP Program

Metodo di isolato<em&gt; Ex Vivo</em&gt; Lung Perfusion in un modello di ratto: Lezioni apprese da Sviluppo di un programma EVLP Rat

The number of acceptable donor lungs available for lung transplantation is severely limited due to poor quality. Ex-Vivo Lung Perfusion (EVLP) has allowed ...

Real-time X-ray Imaging of Lung Fluid Volumes in Neonatal Mouse Lung

In tempo reale a raggi X Imaging del polmone volumi di fluido in neonatale topo polmone

At birth, the lung undergoes a profound phenotypic switch from secretion to absorption, which allows for adaptation to breathing independently. Promoting ...

Apical Resection Mouse Model to Study Early Mammalian Heart Regeneration

Modello apicale resezione mouse per primo studio Mammalian rigenerazione cardiaca

Cardiovascular disease plagues the whole world due to intensive lifestyle changes. Heart regeneration holds great promise for repairing and restoring ...

Open Tracheostomy Gastric Acid Aspiration Murine Model of Acute Lung Injury Results in Maximal Acute Nonlethal Lung Injury

Aprire tracheostomia gastrico acido aspirazione Modello murino di lesioni polmonari acute Risultati in massima acuta non letale Lung Injury

Acid pneumonitis is a major cause of sterile acute lung injury (ALI) in humans. Acid pneumonitis spans the clinical spectrum from asymptomatic to acute ...