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DOI :
10.3791/52333-v
June 17th, 2015
Chapters
0:05
Title
2:02
H9 Cell Culture on Basement Membrane Matrix Coated Plates
4:40
Embryoid Bodies Formation
6:17
Collection of Embryoid Bodies and Treatment
7:55
RNA Isolation
9:31
Splitting and Maintenance of H9 Cells
10:55
Differentiation of hESC towards Neuroectodermal Progenitor Cells (NEP)
15:06
Results: Methyl Mercury Exposure Causes Gene Expression Changes in Embryoid Bodies
16:40
Conclusion
プロトコルは、ヒト胚性幹細胞およびトランスクリプトーム研究に基づいて、2つのin vitro発生毒性試験システム(UKKとUKN1)を記述します。試験系は、ヒトの発生毒性の危険性を予測し、動物実験、コストおよび化学安全性試験のために必要な時間を短縮するために寄与することができます。
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