Name: surgical technique for the implantation of tissue engineered vascular grafts and subsequent in vivo monitoring
Uploaded: 2015-04-03T13:00:00
Description: Watch this Scientific Journal Video about Surgical Technique for the Implantation of Tissue Engineered Vascular Grafts and Subsequent In Vivo Monitoring at JoVE.com

这项工作是由国家心肺研究所（R01 HL086582）和纽约干细胞科学基金（NYSTEM，合同＃资金支持 &#160; C024316），以STA和朱庇特的视频中使用由约翰·奈奎斯特完成DDS插图;医学插画从纽约州立大学布法罗分校。

注：该协议已获得的动物护理和使用委员会在纽约州立大学布法罗分校。 1.预外科制备<ol><li>用羊（赛特交叉，女，约1-3岁，体重40-60公斤）的后续研究。施用环孢菌素A（200毫克/天），阿司匹林（975毫克/天），和香豆定（20-30毫克/天）经口，起始3天手术前，继续对所有研究的持续时间。 </li><li>确保羊已经禁食，术前12小时（正常饲料=1.8公斤干草和0.4公斤粮食）。 </li><li>?...

<ol>
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A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+completely+biological+tissue-engineered+human+blood+vessel.">A completely biological tissue-engineered human blood vessel.</a> The FASEB Journal. 12, 47-56 (1998).</li><li>Samaha, F. J., Oliva, A., Buncke, G. M., Buncke, H. J., Siko, P. 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此报告的目的是提供一种可靠的和可再现的过程来在绵羊颈动脉兴趣植入TEVS。在此模型中使用的动物的天然颈动脉是0.5-0.75毫米厚和4.5-5毫米的外径。这里介绍的手术技术已经成功为植入不同的几何形状测量0.25-1毫米的厚度，4-5毫米外径和4厘米的长度大获成功的TEVS证明是有效的长达3个月期限，预期的终点。使用这种手术技术已使数据采集更容易收集和更一致。 此外，为了测...

对于TEVS发展的首要重点是提供一个替代自体移植替代自体时，船只无法使用，并限制捐助视线的发病率。例如，每年冠状动脉搭桥手术的数量已经超过35万，在美国，和适合移植的理想来源仍然是左乳内动脉，左冠状动脉前降支和大隐静脉1。因为许多个人谁从血管疾病患可能没有合适的动脉和静脉自体移植物置换，TEVS的发展也因此成为研究的强烈场数十年1-6。而新型TEVS工程和优化都发生了很多的进步，所采用植入TEVS自己一直没有这样的激烈讨论的话题的手术技术报告。相反，对于TEVS的植入动物模型的协议在很大程度上离开达研究调查。 下面手稿演示如何植入通过利用端 - 端吻合的方法TEVS。此过程被优化通过使用特定的吻合缝合图案，稳定缝合技术，优化纵向张力并增加体内监测仪器。此方法是对比与一些先前已经使用的许多变型。此外，此过程将介绍如何获得的参数，如动脉血压，TEV直径/合规性和手术，直到外植后通过TEV流量。此数据采集提供了TEV的一个不可缺少的分析，而它在重塑的过程。

<table border="0" cellpadding="0" cellspacing="0" width="1505">
	<tbody>
		<tr height="43">
			<td dir="LTR" height="43" style="height:43px;width:335px;">Equipment</td>
			<td dir="LTR" style="width:208px;">Manufacturer</td>
			<td dir="LTR" style="width:311px;">Serial/Catalog #</td>
			<td dir="LTR" style="width:363px;">Notes</td>
		</tr>
		<tr height="56">
			<td dir="LTR" height="94" rowspan="2" style="height:94px;width:335px;">Pressure Transducer</td>
			<td dir="LTR" rowspan="2" style="width:208px;">Becton Dickinson</td>
			<td dir="LTR" rowspan="2" style="width:311px;">P23XL-1</td>
			<td dir="LTR" style="width:291px;">1+ (1 for each artery) 
			&#160;Used with water-filled diaphragm domes</td>
		</tr>
		<tr height="172">
			<td dir="LTR" height="172" style="height:172px;width:335px;">Amplifier and transducer box</td>
			<td dir="LTR" style="width:208px;">Gould</td>
			<td dir="LTR" style="width:311px;">5900 Signal Conditioner Cage</td>
			<td dir="LTR" style="width:291px;">1 
			Two transducers and amplifiers should be included in cage. While this specific unit may be discontinued, other commercially available pressure transducers with a BNC/analog output will communicate with the Sonometrics equipment</td>
		</tr>
		<tr height="121">
			<td dir="LTR" height="121" style="height:121px;width:335px;">T403 Console with TS420 perivascular flowmeter module(x2)&#160;</td>
			<td dir="LTR" style="width:208px;">Transonic Systems</td>
			<td dir="LTR" style="width:311px;">T403 module and TS420 (x2)</td>
			<td dir="LTR" style="width:291px;">1 
			Flow probes measuring flow through each of the carotid arteries will connect to each of the TS420 units.&#160;&#160;</td>
		</tr>
		<tr height="126">
			<td dir="LTR" height="126" style="height:126px;width:335px;">Digital ultrasonic measurement unit</td>
			<td dir="LTR" style="width:208px;">Sonometrics</td>
			<td dir="LTR" style="width:311px;">TR-USB</td>
			<td dir="LTR" style="width:291px;">1</td>
		</tr>
		<tr height="48">
			<td dir="LTR" height="48" style="height:48px;width:335px;">Flow Probe Precision S-Series 4mm</td>
			<td dir="LTR" rowspan="4" style="width:208px;">Transonic Systems Inc.</td>
			<td dir="LTR" rowspan="4" style="width:311px;">MC4PSS-LS-WC100-CM4B-GA</td>
			<td dir="LTR" rowspan="4" style="width:291px;">2</td>
			<td dir="LTR" rowspan="4" style="width:363px;"></td>
		</tr>
		<tr height="24">
			<td dir="LTR" height="49" rowspan="2" style="height:49px;width:335px;">1mm&#160; Sonometrics Crystals</td>
			<td dir="LTR" rowspan="2" style="width:208px;">Sonometrics Systems</td>
			<td dir="LTR" rowspan="2" style="width:311px;">1R-38S-20-NC-SH</td>
			<td dir="LTR" style="width:291px;">&#160;2-4 (2 for each artery)</td>
		</tr>
		<tr height="81">
			<td dir="LTR" height="134" rowspan="2" style="height:134px;width:335px;">Catheter for implantation</td>
			<td dir="LTR" style="width:208px;">BD (Becton Dickinson)</td>
			<td dir="LTR" rowspan="2" style="width:311px;">&#160;381447</td>
			<td dir="LTR" style="width:291px;">&#160;1+ (1 for each artery) 
			Catheter is cut and secured to microbore tubing, stylette is utilized for insertion&#160;</td>
		</tr>
		<tr height="24">
			<td dir="LTR" height="73" rowspan="2" style="height:73px;width:335px;">Tygon Microbore Tubing</td>
			<td dir="LTR" rowspan="2" style="width:208px;">Norton Performance Plastics</td>
			<td dir="LTR" style="width:311px;">(AAQ04127) Formulation S-54-HL</td>
			<td dir="LTR" style="width:291px;">NA (cut to length for an extension set)</td>
		</tr>
		<tr height="24">
			<td dir="LTR" height="49" rowspan="2" style="height:49px;width:335px;">Luer Stub Adapter</td>
			<td dir="LTR" style="width:208px;">BD (Becton Dickinson)</td>
			<td dir="LTR" style="width:311px;">427564 (20 gauge)</td>
			<td dir="LTR" style="width:291px;">1+ (1 for each arterial catheter)</td>
		</tr>
		<tr height="24">
			<td dir="LTR" height="82" rowspan="2" style="height:82px;width:335px;">Surflo Injection Plug</td>
			<td dir="LTR" rowspan="2" style="width:208px;">Terumo</td>
			<td dir="LTR" rowspan="2" style="width:311px;">SR-IP2</td>
			<td dir="LTR" style="width:291px;">1+ (1 for each arterial catheter)</td>
		</tr>
		<tr height="39">
			<td dir="LTR" height="128" rowspan="2" style="height:128px;width:335px;">Meadox</td>
			<td dir="LTR" rowspan="2" style="width:208px;">PTFE (Teflon) Felt</td>
			<td dir="LTR" rowspan="2" style="width:311px;">19306</td>
			<td dir="LTR" style="width:291px;">NA (cut to size) 
			The PTFE felt used in our studies was discontinued. However, comparable companies such as &#8220;Surgical Mesh&#8221; offer products which are equivalent.&#160;</td>
		</tr>
	</tbody>
</table>

surgical technique for the implantation of tissue engineered vascular grafts and subsequent in vivo monitoring

的组织工程血管（TEVS）的发展是由能力推进到常规和有效地植入TEVS（4-5毫米直径）成一个大的动物模型。一步一步协议为TEV和本机的颈动脉的TEV和实时数字评估间位置放置是由流量探针，导管和超声晶体的植入（能够省略说明。 在体内监测成为可能的记录植入TEVS和本机的颈动脉），在外科手术时的动态直径的变化。一旦植入，研究人员可以计算的动脉血流模式，有创血压和动脉直径产生的参数，如脉搏波传导速度，增强指数，脉压和法规遵从。数据采集​​是利用一个单一的计算机程序，用于在整个实验期间分析来实现的。这种宝贵的数据提供了洞察TEV基质重塑，其resemblanCE到本机/深水控制和体内的整体性能TEV。

30多只羊经历了本报告中的TEVS植入描述（出版）的手术技术9。附表综合方案优化后的最新的羊操作见表2。后TEV植入，没有危及生命的并发症恢复所有的羊。在一些动物中，纤维化，观察在天然动脉留置动脉导管的尖端附近。炎症与加入仪器的存在的显著增加并没有被观察到。很少（1 18的导管），导管引起阻塞通过TEV血流。后一个月发生的这一个障碍。从导管轻微的并发症?...

Watch this Scientific Journal Video about Surgical Technique for the Implantation of Tissue Engineered Vascular Grafts and Subsequent In Vivo Monitoring at JoVE.com

Surgical Technique for the Implantation of Tissue Engineered Vascular Grafts and Subsequent In Vivo Monitoring

一个一步一步的协议，用于组织工程血管（TEVS）到使用终端到终端的吻合和实时数字评估在体内 ，直到动物的牺牲羊的颈动脉间的位置放置。

手术技术组织工程血管移植和后续的植入<em&gt;在体内</em&gt;监控

The development of Tissue Engineered Vessels (TEVs) is advanced by the ability to routinely and effectively implant TEVs (4-5 mm in diameter) into a large ...

Research

JoVE Journal

Bioengineering

Surgical Technique for the Implantation of Tissue Engineered Vascular Grafts and Subsequent In Vivo Monitoring

Bioluminescence Imaging for Assessment of Immune Responses Following Implantation of Engineered Heart Tissue (EHT)

Bioluminescence Imaging for Assessment of Immune Responses Following Implantation of Engineered Heart Tissue EHT

生物发光成像评估的免疫反应，植入心脏组织工程（东隧）

Various techniques of cardiac tissue engineering have been pursued in the past decades including scaffolding strategies using either native or ...

科研

生物工程

Engineering Biological-Based Vascular Grafts Using a Pulsatile Bioreactor

基于生物工程，人造血管使用脉动生物反应器

Much effort has been devoted to develop and advance the methodology to regenerate functional small-diameter arterial bypasses. In the physiological ...

Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy

非接触，无标签使用拉曼光谱研究细胞和细胞外基质的监测

Non-destructive, non-contact and label-free technologies to monitor cell and tissue cultures are needed in the field of biomedical research.1-5 However, ...

Production, Characterization and Potential Uses of a 3D Tissue-engineered Human Esophageal Mucosal Model

三维组织工程人食管黏膜型号生产，表征和潜在用途

The incidence of both esophageal adenocarcinoma and its precursor, Barrett&#8217;s Metaplasia, are rising rapidly in the western world. Furthermore ...

Athymic Rat Model for Evaluation of Engineered Anterior Cruciate Ligament Grafts

无胸​​腺大鼠模型工程前交叉韧带移植评估

Anterior cruciate ligament (ACL) rupture is a common ligamentous injury that often requires surgery because the ACL does not heal well without ...

Engineering 3D Cellularized Collagen Gels for Vascular Tissue Regeneration

工程三维细胞化胶原凝胶的血管组织再生

Synthetic materials are known to initiate clinical complications such as inflammation, stenosis, and infections when implanted as vascular substitutes. ...

Applications of In Vivo Functional Testing of the Rat Tibialis Anterior for Evaluating Tissue Engineered Skeletal Muscle Repair

Applications of In Vivo Functional Testing of the Rat Tibialis Anterior for Evaluating Tissue Engineered Skeletal Muscle Repair

中的应用<em&gt;在体内</em&gt;评定组织的鼠胫骨前的功能测试工程化骨骼肌修复

Despite the regenerative capacity of skeletal muscle, permanent functional and/or cosmetic deficits (e.g., volumetric muscle loss (VML) resulting from ...