Name: surgical technique for the implantation of tissue engineered vascular grafts and subsequent in vivo monitoring
Uploaded: 2015-04-03T13:00:00
Description: Watch this Scientific Journal Video about Surgical Technique for the Implantation of Tissue Engineered Vascular Grafts and Subsequent In Vivo Monitoring at JoVE.com

Questo lavoro è stato sostenuto da sovvenzioni dal Cuore e Polmone Istituto Nazionale (R01 HL086582) e la Stem Cell Science Fund di New York (NYSTEM, Contract # &#160; C024316) per STA e DDS illustrazioni utilizzate in video JoVE sono state completate da John Nyquist; Medical Illustrator dalla State University di New York a Buffalo.

NOTA: Questo protocollo è stato approvato dalla cura e l&#39;uso degli animali Comitato presso la State University di New York a Buffalo. Preparazione 1. Pre-chirurgica <ol><li> Utilizzare pecore (Dorset croce, femmina, circa 1-3 anni con un peso di 40-60 kg) per il seguente studio. Somministrare ciclosporina A (200 mg / die), aspirina (975 mg / die), e coumadin (20-30 mg / die) per via orale, iniziando 3 giorni prima chirurgia e continua per tutta la durata di tutti gli studi. </li><li> ...

<ol>
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G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+fate+of+an+endothelium+layer+after+preconditioning.">The fate of an endothelium layer after preconditioning.</a> Journal of vascular surgery : Official Publication, the Society for Vascular Surgery [and] International Society for Cardiovascular Surgery, North American Chapter. 51, 174-183 (2010).</li><li>Ueberrueck, T., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Comparison+of+the+ovine+and+porcine+animal+models+for+biocompatibility+testing+of+vascular+prostheses.">Comparison of the ovine and porcine animal models for biocompatibility testing of vascular prostheses.</a> Journal of Surgical Research. 124, 305-311 (2005).</li><li>Labb&#233;, R., Germain, L., Auger, F. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+completely+biological+tissue-engineered+human+blood+vessel.">A completely biological tissue-engineered human blood vessel.</a> The FASEB Journal. 12, 47-56 (1998).</li><li>Samaha, F. J., Oliva, A., Buncke, G. M., Buncke, H. J., Siko, P. 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Lo scopo di questo rapporto è quello di fornire una procedura affidabile e riproducibile di TEV impianto di interesse nella carotide ovine. Le arterie carotidi native degli animali utilizzati in questo modello sono stati 0,5-0,75 mm di spessore e 4,5-5 mm di diametro esterno. La tecnica chirurgica qui descritta ha avuto successo per l&#39;impianto di TEV di varie geometrie di misura 0,25-1 mm di spessore, 4-5 mm di diametro esterno e 4 cm di lunghezza con grande successo dimostrando efficace fino a 3 mesi di durata, il...

L&#39;obiettivo principale per lo sviluppo di TEV è stato quello di fornire un sostituto per la sostituzione del trapianto autologo quando vasi autologhi non sono disponibili e limitare donatori di vista morbilità. Ad esempio, il numero di interventi di bypass coronarico all&#39;anno ha superato 350.000 negli Stati Uniti, e la fonte ideale di opportuni innesti rimane fianco arteria mammaria interna, discendente anteriore dell&#39;arteria coronaria e safena 1. Dal momento che molte persone che soffrono di malattie vascolari non possono avere le arterie e le vene adatte per la sostituzione del trapianto autologo, lo sviluppo di TEV è così diventato un campo di intensa ricerca da decenni 1-6. Mentre la progettazione e l&#39;ottimizzazione di nuovi TEV hanno subito molti progressi, relazioni sulle tecniche chirurgiche impiegate per impiantare i TEV stessi non è stato un argomento di tale discussione intensa. Piuttosto, i protocolli per quanto riguarda l&#39;impianto di TEV in modelli animali sono in gran parte lasciatifino alla ricerca investigatori. Il seguente manoscritto illustrato come impiantare TEV utilizzando un approccio anastomosi end-to-end. Questa procedura è stata ottimizzata utilizzando uno specifico modello di sutura anastomotica, stabilizzante tecnica di sutura, ottimizzando tensione longitudinale e l&#39;aggiunta di monitoraggio in vivo strumentazione. Questo metodo è in contrasto con alcune delle molte varianti che sono state usate in precedenza. Inoltre, questa procedura viene descritto come acquisire i parametri come la pressione arteriosa, TEV diametro / conformità e portata attraverso il TEV dopo l&#39;intervento fino espianto. Questa raccolta di dati fornisce un&#39;analisi indispensabile del TEV mentre è in fase di rimodellamento.

<table border="0" cellpadding="0" cellspacing="0" width="1505">
	<tbody>
		<tr height="43">
			<td dir="LTR" height="43" style="height:43px;width:335px;">Equipment</td>
			<td dir="LTR" style="width:208px;">Manufacturer</td>
			<td dir="LTR" style="width:311px;">Serial/Catalog #</td>
			<td dir="LTR" style="width:363px;">Notes</td>
		</tr>
		<tr height="56">
			<td dir="LTR" height="94" rowspan="2" style="height:94px;width:335px;">Pressure Transducer</td>
			<td dir="LTR" rowspan="2" style="width:208px;">Becton Dickinson</td>
			<td dir="LTR" rowspan="2" style="width:311px;">P23XL-1</td>
			<td dir="LTR" style="width:291px;">1+ (1 for each artery) 
			&#160;Used with water-filled diaphragm domes</td>
		</tr>
		<tr height="172">
			<td dir="LTR" height="172" style="height:172px;width:335px;">Amplifier and transducer box</td>
			<td dir="LTR" style="width:208px;">Gould</td>
			<td dir="LTR" style="width:311px;">5900 Signal Conditioner Cage</td>
			<td dir="LTR" style="width:291px;">1 
			Two transducers and amplifiers should be included in cage. While this specific unit may be discontinued, other commercially available pressure transducers with a BNC/analog output will communicate with the Sonometrics equipment</td>
		</tr>
		<tr height="121">
			<td dir="LTR" height="121" style="height:121px;width:335px;">T403 Console with TS420 perivascular flowmeter module(x2)&#160;</td>
			<td dir="LTR" style="width:208px;">Transonic Systems</td>
			<td dir="LTR" style="width:311px;">T403 module and TS420 (x2)</td>
			<td dir="LTR" style="width:291px;">1 
			Flow probes measuring flow through each of the carotid arteries will connect to each of the TS420 units.&#160;&#160;</td>
		</tr>
		<tr height="126">
			<td dir="LTR" height="126" style="height:126px;width:335px;">Digital ultrasonic measurement unit</td>
			<td dir="LTR" style="width:208px;">Sonometrics</td>
			<td dir="LTR" style="width:311px;">TR-USB</td>
			<td dir="LTR" style="width:291px;">1</td>
		</tr>
		<tr height="48">
			<td dir="LTR" height="48" style="height:48px;width:335px;">Flow Probe Precision S-Series 4mm</td>
			<td dir="LTR" rowspan="4" style="width:208px;">Transonic Systems Inc.</td>
			<td dir="LTR" rowspan="4" style="width:311px;">MC4PSS-LS-WC100-CM4B-GA</td>
			<td dir="LTR" rowspan="4" style="width:291px;">2</td>
			<td dir="LTR" rowspan="4" style="width:363px;"></td>
		</tr>
		<tr height="24">
			<td dir="LTR" height="49" rowspan="2" style="height:49px;width:335px;">1mm&#160; Sonometrics Crystals</td>
			<td dir="LTR" rowspan="2" style="width:208px;">Sonometrics Systems</td>
			<td dir="LTR" rowspan="2" style="width:311px;">1R-38S-20-NC-SH</td>
			<td dir="LTR" style="width:291px;">&#160;2-4 (2 for each artery)</td>
		</tr>
		<tr height="81">
			<td dir="LTR" height="134" rowspan="2" style="height:134px;width:335px;">Catheter for implantation</td>
			<td dir="LTR" style="width:208px;">BD (Becton Dickinson)</td>
			<td dir="LTR" rowspan="2" style="width:311px;">&#160;381447</td>
			<td dir="LTR" style="width:291px;">&#160;1+ (1 for each artery) 
			Catheter is cut and secured to microbore tubing, stylette is utilized for insertion&#160;</td>
		</tr>
		<tr height="24">
			<td dir="LTR" height="73" rowspan="2" style="height:73px;width:335px;">Tygon Microbore Tubing</td>
			<td dir="LTR" rowspan="2" style="width:208px;">Norton Performance Plastics</td>
			<td dir="LTR" style="width:311px;">(AAQ04127) Formulation S-54-HL</td>
			<td dir="LTR" style="width:291px;">NA (cut to length for an extension set)</td>
		</tr>
		<tr height="24">
			<td dir="LTR" height="49" rowspan="2" style="height:49px;width:335px;">Luer Stub Adapter</td>
			<td dir="LTR" style="width:208px;">BD (Becton Dickinson)</td>
			<td dir="LTR" style="width:311px;">427564 (20 gauge)</td>
			<td dir="LTR" style="width:291px;">1+ (1 for each arterial catheter)</td>
		</tr>
		<tr height="24">
			<td dir="LTR" height="82" rowspan="2" style="height:82px;width:335px;">Surflo Injection Plug</td>
			<td dir="LTR" rowspan="2" style="width:208px;">Terumo</td>
			<td dir="LTR" rowspan="2" style="width:311px;">SR-IP2</td>
			<td dir="LTR" style="width:291px;">1+ (1 for each arterial catheter)</td>
		</tr>
		<tr height="39">
			<td dir="LTR" height="128" rowspan="2" style="height:128px;width:335px;">Meadox</td>
			<td dir="LTR" rowspan="2" style="width:208px;">PTFE (Teflon) Felt</td>
			<td dir="LTR" rowspan="2" style="width:311px;">19306</td>
			<td dir="LTR" style="width:291px;">NA (cut to size) 
			The PTFE felt used in our studies was discontinued. However, comparable companies such as &#8220;Surgical Mesh&#8221; offer products which are equivalent.&#160;</td>
		</tr>
	</tbody>
</table>

surgical technique for the implantation of tissue engineered vascular grafts and subsequent in vivo monitoring

Lo sviluppo di ingegneria tessutale Vessels (TEV) è avanzata dalla capacità di di routine ed efficace TEV impianto (4-5 mm di diametro) in un grande modello animale. Un protocollo passo-passo per il posizionamento tra posizionale della valutazione TEV digitale in tempo reale del TEV e arterie carotidi native è descritto qui. In vivo monitoraggio è reso possibile dal impiantazione di sonde di flusso, cateteri e cristalli ultrasonici (grado di registrare i cambiamenti di diametro dinamici di TEV impiantati e delle arterie carotidi native) al momento della chirurgia. Una volta impiantato, i ricercatori possono calcolare i modelli flusso di sangue arterioso, invasiva della pressione arteriosa e del diametro dell&#39;arteria che producono parametri come la velocità dell&#39;onda di polso, indice di aumento, le pressioni di impulsi e la conformità. L&#39;acquisizione dei dati viene eseguita utilizzando un singolo programma per computer per l&#39;analisi per tutta la durata dell&#39;esperimento. Tali dati inestimabile permette di comprendere meglio TEV rimodellamento della matrice, la sua resemblanCé a controlli nativi / sham e prestazioni TEV generale in vivo.

Più di 30 pecore sono stati sottoposti alla tecnica chirurgica descritta nella presente relazione per l&#39;impianto di TEV (in corso di stampa) 9. Una tabella che riassume le più recenti operazioni di ovini dopo l&#39;ottimizzazione del protocollo sono riportati nella tabella 2. Tutte le pecore recuperati dopo TEV l&#39;impianto senza pericolo di vita complicazioni. In alcuni animali, la fibrosi è stata osservata in arteria nativa vicino alla punta del catetere arterioso. Non è stato oss...

Watch this Scientific Journal Video about Surgical Technique for the Implantation of Tissue Engineered Vascular Grafts and Subsequent In Vivo Monitoring at JoVE.com

Surgical Technique for the Implantation of Tissue Engineered Vascular Grafts and Subsequent In Vivo Monitoring

Un protocollo step-by-step per il collocamento inter-posizionale di ingegneria tessutale Vessels (TEV) nella carotide di una pecora con anastomosi end-to-end e di valutazione digitale in tempo reale in vivo fino al sacrificio degli animali.

Tecnica chirurgica per l&#39;impianto di ingegneria tessutale vascolari Innesti e successive<em&gt; In Vivo</em&gt; Monitoraggio

The development of Tissue Engineered Vessels (TEVs) is advanced by the ability to routinely and effectively implant TEVs (4-5 mm in diameter) into a large ...

Research

JoVE Journal

Bioengineering

Surgical Technique for the Implantation of Tissue Engineered Vascular Grafts and Subsequent In Vivo Monitoring

Bioluminescence Imaging for Assessment of Immune Responses Following Implantation of Engineered Heart Tissue (EHT)

Bioluminescence Imaging for Assessment of Immune Responses Following Implantation of Engineered Heart Tissue EHT

Imaging bioluminescenza per la valutazione delle risposte immunitarie A seguito di impianto di tessuto cardiaco Composti (EHT)

Various techniques of cardiac tissue engineering have been pursued in the past decades including scaffolding strategies using either native or ...

Ricerca

Bioingegneria

Engineering Biological-Based Vascular Grafts Using a Pulsatile Bioreactor

Ingegneria biologica basata innesti vascolari Utilizzando un bioreattore pulsatile

Much effort has been devoted to develop and advance the methodology to regenerate functional small-diameter arterial bypasses. In the physiological ...

Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy

Senza contatto, Label-free monitoraggio delle cellule e matrice extracellulare mediante Spettroscopia Raman

Non-destructive, non-contact and label-free technologies to monitor cell and tissue cultures are needed in the field of biomedical research.1-5 However, ...

Production, Characterization and Potential Uses of a 3D Tissue-engineered Human Esophageal Mucosal Model

Produzione, caratterizzazione e possibilità di utilizzo di un 3D tissutale umano esofageo mucose Modello

The incidence of both esophageal adenocarcinoma and its precursor, Barrett&#8217;s Metaplasia, are rising rapidly in the western world. Furthermore ...

Athymic Rat Model for Evaluation of Engineered Anterior Cruciate Ligament Grafts

Atimico Rat modello per la valutazione di Engineered legamento crociato anteriore Innesti

Anterior cruciate ligament (ACL) rupture is a common ligamentous injury that often requires surgery because the ACL does not heal well without ...

Engineering 3D Cellularized Collagen Gels for Vascular Tissue Regeneration

Ingegneria 3D Cellularized collagene Gel per Vascolare Rigenerazione Tissutale

Synthetic materials are known to initiate clinical complications such as inflammation, stenosis, and infections when implanted as vascular substitutes. ...

Applications of In Vivo Functional Testing of the Rat Tibialis Anterior for Evaluating Tissue Engineered Skeletal Muscle Repair

Applications of In Vivo Functional Testing of the Rat Tibialis Anterior for Evaluating Tissue Engineered Skeletal Muscle Repair

applicazioni di<em&gt; In Vivo</em&gt; Test funzionale del Topo tibiale anteriore per la valutazione di ingegneria tessutale muscolo scheletrico di riparazione

Despite the regenerative capacity of skeletal muscle, permanent functional and/or cosmetic deficits (e.g., volumetric muscle loss (VML) resulting from ...