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11.4K Views
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09:00 min
March 18th, 2015
DOI :
10.3791/52355-v
Chapters
0:05
Title
1:44
RNA Transcription with Corrole Treatment
3:09
Agarose Gel Electrophoresis (1% Agarose Gel)
5:58
RNA Quantification via UV-Vis Spectroscopy
6:47
Results: Assessment of RNA Transcription by Agarose Gel Electrophoresis and UV-Vis Spectroscopy
8:17
Conclusion
Summary
镓(Ⅲ)5,10,15-(三)pentafluorophenylcorrole和其游离碱类似物显示出低的微摩尔细胞的细胞毒性。这个手稿描述的RNA转录反应中,成像的RNA用溴化乙锭染色的凝胶,并用UV-Vis光谱进行定量的RNA,为了通过corroles评估转录抑制和演示评价抗癌候选属性的一个简单的方法。
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