Name: rat model of photochemically-induced posterior ischemic optic neuropathy
Uploaded: 2015-11-29T14:00:00
Description: Watch this Scientific Journal Video about Rat Model of Photochemically-Induced Posterior Ischemic Optic Neuropathy at JoVE.com

我们感谢Eleut埃尔南德斯畜牧业，加布Gaidosh显微镜的专业知识，以及奎Tran和镇扬赵视频编辑。该研究由美国国家眼科研究所授予R01-EY022129到JLG和P30补助EY022589到加州大学圣地亚哥分校和EY014801到UM;美国心脏协会，詹姆斯和Esther国王基金会，复旦大学研究生院（编号2010033）的博士研究生交流计划资金，以及无限制的赠款防盲研究公司

所有动物的程序批准了加州大学圣地亚哥和迈阿密体制动物护理和使用委员会（IACUC）大学等按照ARVO声明对动物的眼科和视觉研究中的应用进行。所有试剂和外科手术使用的仪器是不育的。 1.麻醉和准备鼠外科手术<ol><li>前程序，将大鼠按照体重麻醉腹膜内注射氯胺酮（60毫克/千克）和赛拉嗪（8毫克/千克）的。麻醉足够的深度应到脚趾捏刺激了否定的答复来确定。 </li><li>麻醉后，将舌头?...

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Here we describe in detail a method for inducing PION in a rat model. The most critical part of the protocol is the exposure and irradiation of the optic nerve &#8211; to expose the nerve as long as possible while avoiding damage caused by the sharp fine tip forceps or from stretching. In rats, the ophthalmic artery enters the optic nerve &#8804;1 mm from the optic nerve head. Therefore, irradiation of the optic nerve 3-4 mm away from the optic nerve head should only result in ischemia of the capillaries feeding the nerv...

患者50岁，缺血性视神经病变（ION）是最常见的类型的急性视神经病变2。前（AION）或后（π介子）3：条件可根据具体的受影响的血液供给和临床上介绍的源呈现为一个两种亚型的。虽然AION的发病机理和过程已被广泛研究4-7，PION一直由于其低流行，变呈现，不明确的诊断标准，缺乏动物模型知之甚少。此外，没有任何的治疗已被证明有效地防止或从AION或π介子反向视力丧失。因此，π介子可重复的和可靠的动物模型是很有价值的研究在体内的疾病过程和神经保护和轴突再生测试新的治疗方案。 化学诱发缺血性损伤导致vasog微血管ENIC水肿和血栓形成有效地创建区域组织缺血8-12。注射到血管循环后，光敏染料赤藓红B最终产生在激活活性单线氧分子靶血管激光照射。单重态氧直接peroxidizes血管内皮，刺激血小板粘附/聚集并导致闭塞性血栓的形成。缺血性损伤蔓延到邻近地区和微血管压迫因血管性水肿进一步加剧。此协议的总体目标是光化学诱发缺血的球后视神经镜像造成PION的损害。 据我们所知，这是在后视神经1缺血性损伤的第一模型。由于这种模式产生缺血，同时避免身体创伤，对后部缺血性视神经病变的生理过程是更好的模仿和学习。此外，这种模式提供了一个新的平台，为候选人为治疗视神经病变等中枢神经系统缺血性疾病的筛查。在这里，股静脉置管，视神经接触，静脉注射赤藓红的B和激光照射大鼠模型PION了详细的方案进行了说明。

<table>
	<tbody>
		<tr>
			<td>532-nm Nd:YAG laser&#160;</td>
			<td>Laser glow</td>
			<td>LRS-532-KM-200-3</td>
			<td></td>
		</tr>
		<tr>
			<td>Beam chopper</td>
			<td>custom-made</td>
			<td>custom-made</td>
			<td></td>
		</tr>
		<tr>
			<td>Mechanical shutter and corresponding shutter drive timer</td>
			<td>AAM Vincent Associates</td>
			<td>SD-10</td>
			<td></td>
		</tr>
		<tr>
			<td>25-cm focal length spherical lens</td>
			<td>CVI/Mellles-Griot</td>
			<td>01 LPX 293</td>
			<td>plano-convexBK7 glass lens with HEBBARTM antireflection coating</td>
		</tr>
		<tr>
			<td>Erythrosin B&#160;</td>
			<td>MP Biomedicals</td>
			<td>190449</td>
			<td></td>
		</tr>
		<tr>
			<td>Fluorogold</td>
			<td>Fluorochrome,LLC</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Gelfoam</td>
			<td>Cardinal Health</td>
			<td>CAH1203421</td>
			<td></td>
		</tr>
		<tr>
			<td>Polyethylene tubing (PE10)</td>
			<td>BD Intramedic</td>
			<td>427400</td>
			<td></td>
		</tr>
		<tr>
			<td>No. 10 Blade</td>
			<td>Miltex</td>
			<td>4-110</td>
			<td></td>
		</tr>
		<tr>
			<td>Fine Forceps</td>
			<td>F.S.T.</td>
			<td>91150-20</td>
			<td>DUMONT #5 RUSTLESS NON-MAGNETIC</td>
		</tr>
		<tr>
			<td>Forceps with Teeth</td>
			<td>F.S.T.</td>
			<td>11153-10</td>
			<td>Germany stainless</td>
		</tr>
		<tr>
			<td>Forceps</td>
			<td>F.S.T.</td>
			<td>18025-10&#160;</td>
			<td>Germany stainless</td>
		</tr>
		<tr>
			<td>Vannas spring scissors</td>
			<td>F.S.T.</td>
			<td>2-220&#160;</td>
			<td>JJECK Stainless</td>
		</tr>
		<tr>
			<td>Polyglactin suture</td>
			<td>Ethicon</td>
			<td>J488G</td>
			<td>7-0 suture</td>
		</tr>
		<tr>
			<td>hemostat</td>
			<td>F.S.T.</td>
			<td>12075-12&#160;</td>
			<td>Germany stainless</td>
		</tr>
	</tbody>
</table>

rat model of photochemically-induced posterior ischemic optic neuropathy

后部缺血性视神经病变（PION）是一见倾心，破坏性疾病的临床实践。然而，其发病机制与自然史仍然知之甚少。最近，我们开发PION的一个可靠的，可再现的动物模型和测试在这个模型1的一些神经营养因子的治疗效果。该视频的目的是表明我们光化学诱导的后部缺血性视神经病变模型，并评估其与视网膜神经节细胞的逆行标记作用。以下后视神经，光敏染料，赤藓红乙手术暴露，静脉注射和将激光束聚焦到视神经表面。赤藓红B和激光的期间，照射损伤血管内皮，促使微血管闭塞血小板血栓和水肿压缩介导的光化学相互作用。由此产生的缺血性损伤产生一个渐进的，但pronounc编视网膜神经节细胞枯死，由于亏损轴索输入 - 远程，损伤诱发和临床相关的结果。因此，这种模型提供了一种新颖的平台来研究PION的病理生理过程中，而且可用于测试治疗方法为视神经病变以及其它CNS缺血性疾病进一步优化。

诱导这种技术将所得的缺血性损伤的产生视网膜神经节细胞轴突缺血损伤后逐渐但明显的死亡。这类似于在人类疾病观察到临床上相关的结果。 FG逆行标记用于PION后量化RGC存活。相同的方法被用来验证一个模型的创建成功以及评估不同治疗方案的效果。 图1示出 FG阳性细胞的代表性共聚焦图像在视网膜平面坐骑控制（图1A），假治疗（激光只/无赤藓红B， 图1B），</s...

Watch this Scientific Journal Video about Rat Model of Photochemically-Induced Posterior Ischemic Optic Neuropathy at JoVE.com

Rat Model of Photochemically-Induced Posterior Ischemic Optic Neuropathy

该协议的目的是光化学诱导缺血性损伤至大鼠后视神经。这个模型是至关重要的后部缺血性视神经病变的病理生理的研究，以及治疗方法，这和其他视神经病变，以及其它CNS缺血性疾病。

光化学诱导后部缺血性视神经病变的大鼠模型

Posterior Ischemic optic neuropathy (PION) is a sight-devastating disease in clinical practice. However, its pathogenesis and natural history ...

Research

JoVE Journal

Medicine

Creation of Reversible Cholestatic Rat Model

可逆的胆汁淤积大鼠模型的建立

Cholestasis is a clinical condition commonly encountered by both surgeons and gastroenterologists. Cholestasis can cause various physiological changes and ...

科研

医学

Gene Transfer for Ischemic Heart Failure in a Preclinical Model

缺血性心力衰竭在临床前模型的转基因

Various emerging technologies are being developed for patients with heart failure. Well-established preclinical evaluations are necessary to determine ...

A Simplified Technique for Producing an Ischemic Wound Model

一个生产缺血性损伤模型的简化技术

One major obstacle in current diabetic wound research is a lack of an ischemic wound model that can be safely used in diabetic animals. Drugs that work ...

Endothelin-1 Induced Middle Cerebral Artery Occlusion Model for Ischemic Stroke with Laser Doppler Flowmetry Guidance in Rat

内皮素-1诱导大脑中动脉闭塞模型缺血性中风激光多普勒血流仪在大鼠指导

Stroke is the number one cause of disability and third leading cause of death in the world, costing an estimated $70 billion in the United States in ...

Angiogenesis in the Ischemic Rat Lung

在缺血大鼠肺的血管生成

The  adult lung is perfused by both the systemic bronchial artery and the entire  venous return flowing through the pulmonary arteries. In most lung ...

A Piglet Model of Neonatal Hypoxic-Ischemic Encephalopathy

新生儿缺氧缺血性脑病的小猪模型

Birth asphyxia, which causes hypoxic-ischemic encephalopathy (HIE), accounts for 0.66 million deaths worldwide each year, about a quarter of the ...

Demonstration of the Rat Ischemic Skin Wound Model

在大鼠缺血性皮肤创伤模型的演示

The propensity for chronic wounds in humans increases with ageing, disease conditions such as diabetes and impaired cardiovascular function, and ...

The Rodent Model of Nonarteritic Anterior Ischemic Optic Neuropathy (rNAION)

The Rodent Model of Nonarteritic Anterior Ischemic Optic Neuropathy rNAION

非动脉炎性前部缺血性视神经病变的啮齿动物模型（rNAION）

Nonarteritic anterior ischemic optic neuropathy (NAION) is a focal ischemic lesion of the optic nerve that affects 1/700 individuals throughout their ...

Isometric Contractility Measurement of the Mouse Mesenteric Artery Using Wire Myography

用线 Myography 测定小鼠肠系膜动脉的等轴收缩力

The wire myograph technique is used to assess the contractility of vascular smooth muscles in response to depolarization, GPCR agonists/inhibitors and ...

Oxygen-Induced Retinopathy Model for Ischemic Retinal Diseases in Rodents

罗登斯缺血性视网膜疾病的氧诱导视网膜病变模型

One of the commonly used models for ischemic retinopathies is the oxygen-induced retinopathy (OIR) model. Here we describe detailed protocols for the OIR ...