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واللونية الفحص أن يقيس على وجه التحديد آخر باء granzyme متعلق ب التحلل البروتيني: التحلل من بوك علاء علاء-ASP-S-BZL

DOI :

10.3791/52419-v

November 28th, 2014

November 28th, 2014

12,784 Views

1Cancer Immunology Program, Peter MacCallum Cancer Centre

We describe a simple, quantitative colorimetric assay that specifically measures the proteolytic activity of human, mouse or rat Granzyme B (GzmB). This protocol can be easily adapted for determining protease activity of other granule serine proteases by the hydrolysis of other synthetic peptide substrates with an appropriate recognition sequence.

Tags

93 granzyme thioesters BOC ASP S BZL

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