比色测定的具体措施颗粒酶B蛋白水解活动：水解的Boc-丙氨酸 - 丙氨酸-ASP-S-BZL的

We describe a simple, quantitative colorimetric assay that specifically measures the proteolytic activity of human, mouse or rat Granzyme B (GzmB). This protocol can be easily adapted for determining protease activity of other granule serine proteases by the hydrolysis of other synthetic peptide substrates with an appropriate recognition sequence.