Boc-अला-अला-ASP-एस-Bzl की hydrolysis: एक वर्णमिति विशेष रूप से granzyme बी proteolytic गतिविधि उपाय है कि परख

We describe a simple, quantitative colorimetric assay that specifically measures the proteolytic activity of human, mouse or rat Granzyme B (GzmB). This protocol can be easily adapted for determining protease activity of other granule serine proteases by the hydrolysis of other synthetic peptide substrates with an appropriate recognition sequence.