Name: mechanical vessel injury in zebrafish embryos
Uploaded: 2015-02-17T14:00:00
Description: Watch this Scientific Journal Video about Mechanical Vessel Injury in Zebrafish Embryos at JoVE.com

The authors would like to thank Drs. Stephen Wilson and Lisa Wilsbacher for helpful discussions. This work was supported in part by NIH HL054737.

وتمت الموافقة على إجراءات باستخدام الزرد التي كتبها المؤسسي رعاية الحيوان UCSF واللجنة الاستخدام: ملاحظة. 1. إعداد أدوات <ol style=";text-align:right;direction:rtl"><li style=";text-align:right;direction:rtl"> إدراج التفاصيل التافهة دبوس إلى حامل دبوس المشبك ودبوس. </li><l...

<ol>
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وقد استخدمت بنجاح الزرد كنموذج لأنواع مختلفة من الجروح بما في ذلك الضرر الليزر 13-15، التي يسببها الليزر تخثر 16، والظهارية اصابة 10. نحن التقرير وسيلة لإصابة الميكانيكية التي هي بسيطة لتنفيذ وتنتج الإصابة التي تسيطر عليها في نموذج في الجسم الحي ا?...

Zebrafish have been used extensively to study a variety of topics in vascular biology, including vascular development, angiogenesis, and hematopoietic development and pathology1-3. Embryos develop a functional circulation as well as leukocytes and other components of the innate immune system by 1 day post fertilization (dpf) 1,4,5. The conservation of the inflammatory and leukocyte response to injury has made the zebrafish embryo an informative model for such diverse inflammatory processes as tuberculous infection, enterocolitis, and tissue regeneration6-9. Zebrafish embryos have been used to study injury-related inflammation particularly in the context of epithelial wounding and the neutrophil response10,11. Injury to the embryo results in a highly conserved cellular response from cells at the injury site and the innate immune cells recruited to respond to the injury and regulate its resolution11,12. Other injury models have used focused laser pulses to spatially localize injury to specific cell types including neurons, muscle cells, and cardiomyocytes13-15. 
Zebrafish embryos have been used as a model to study hemostasis and thrombosis in conditions of pharmacological and genetic manipulation, using both mechanical and laser-induced thrombus formation16-19. Components of the coagulation cascade appear to be well-conserved and transgenics have allowed for detailed studies of thrombocyte and fibrin deposition at the site of coagulation17,20,21. The procedure presented in this paper complements these methods by providing a system for studying mechanical vessel injury resulting in vessel breach, thrombus formation and resolution, and vessel repair.

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			<td height="21" style="height:21px;width:248px;">Insulin syringe with 28G1/2 needle</td>
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			<td style="width:159px;">329461</td>
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mechanical vessel injury in zebrafish embryos

Zebrafish (Danio rerio) embryos have proven to be a powerful model for studying a variety of developmental and disease processes. External development and optical transparency make these embryos especially amenable to microscopy, and numerous transgenic lines that label specific cell types with fluorescent proteins are available, making the zebrafish embryo an ideal system for visualizing the interaction of vascular, hematopoietic, and other cell types during injury and repair in vivo. Forward and reverse genetics in zebrafish are well developed, and pharmacological manipulation is possible. We describe a mechanical vascular injury model using micromanipulation techniques that exploits several of these features to study responses to vascular injury including hemostasis and blood vessel repair. Using a combination of video and timelapse microscopy, we demonstrate that this method of vascular injury results in measurable and reproducible responses during hemostasis and wound repair. This method provides a system for studying vascular injury and repair in detail in a whole animal model.

تم تنفيذ إصابة سفينة الميكانيكية في 2 DPF الأجنة (الشكل 2A - C). النتائج إصابة في استجابة سريعة وموثوق بها تخثر مقاسا الوقت لوقف النزيف (الشكل 2D). لتحديد ما إذا كان الاختلاف في الاستجابة التخثر يمكن قياسها، كانت تدار من هيرودين تخثر إلى الأجنة عن...

Watch this Scientific Journal Video about Mechanical Vessel Injury in Zebrafish Embryos at JoVE.com

Mechanical Vessel Injury in Zebrafish Embryos

This article describes a method for creating a mechanical vessel injury in zebrafish embryos. This injury model provides a platform for studying hemostasis, injury-related inflammation, and wound healing in an organism ideally suited for real-time microscopy.

إصابة سفينة الميكانيكية في الزرد الأجنة

Zebrafish (Danio rerio) embryos have proven to be a powerful model for studying a variety of developmental and disease processes.  External development ...

Research

JoVE Journal

Developmental Biology

Kidney Regeneration in Adult Zebrafish by Gentamicin Induced Injury

التجديد الكلى في اسماك الزرد الكبار قبل جنتاميسين المستحثة الإصابات

The kidney is essential for fluid homeostasis, blood pressure regulation and filtration of waste from the body. The fundamental unit of kidney function is ...

Isolation and Characterization of Single Cells from Zebrafish Embryos

عزل وتوصيف الخلايا واحدة من اسماك الزرد الأجنة

The zebrafish (Danio rerio) is a powerful model organism to study vertebrate development. Though many aspects of zebrafish embryonic development have been ...

Affinity Labeling Detection of Endogenous Receptors from Zebrafish Embryos

تقارب كشف وسمها الذاتية المستقبلات من الزرد الأجنة

By combining the powers of Affinity Labeling and Immunoprecipitation (AFLIP), a technique for the detection of low abundance receptors in zebrafish ...

Ploidy Manipulation of Zebrafish Embryos with Heat Shock 2 Treatment

التلاعب الصيغة الصبغية من الزرد الأجنة مع الحرارة صدمة 2 العلاج

Manipulation of ploidy allows for useful transformations, such as diploids to tetraploids, or haploids to diploids. In the zebrafish Danio rerio, ...

Biosensing Motor Neuron Membrane Potential in Live Zebrafish Embryos

بيوسنسينغ موتور الخلايا العصبية الغشاء المحتملة في الأجنة الزرد الحية

The protocols described here are designed to allow researchers to study cell communication without altering the integrity of the environment in which the ...

Precise Cellular Ablation Approach for Modeling Acute Kidney Injury in Developing Zebrafish

دقة الخلوية طريقة الاجتثاث لنمذجة إصابة الكلى الحادة في تطوير الزرد

Acute Kidney Injury (AKI) is a common medical condition with a high mortality rate. With the repair abilities of the kidney, it is possible to restore ...

Histological Analyses of Acute Alcoholic Liver Injury in Zebrafish

التحاليل النسيجية لإصابات الكبد الكحولية الحادة في الزرد

Alcoholic Liver Disease (ALD) refers to damage to the liver due to acute or chronic alcohol abuse. It is among the leading causes of alcohol-related ...

Induction of Hypoxia in Living Frog and Zebrafish Embryos

تحريض نقص الأكسجة في الضفدع الحي والأجنة الزرد

Here, we introduce a novel system for hypoxia induction, which we developed to study the effects of hypoxia in aquatic organisms such as frog and ...

Visualization of Cellular Electrical Activity in Zebrafish Early Embryos and Tumors

التصور للنشاط الكهربائي الخلوية في الزرد الأجنة في وقت مبكر، والأورام

Bioelectricity, endogenous electrical signaling mediated by ion channels and pumps located on the cell membrane, plays important roles in signaling ...

Whole Mount Immunohistochemistry in Zebrafish Embryos and Larvae

كامل جبل المناعة الكيمياء في الأجنة حمار وحشي واليرقات

Immunohistochemistry is a widely used technique to explore protein expression and localization during both normal developmental and disease states. ...