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DOI :
10.3791/52465-v
February 17th, 2015
Chapters
0:05
Title
1:19
Differentiation Procedure
4:56
Selection of Sinus Nodal Cells
7:12
Results: Generation of Cardiac Pacemaker Cell Aggregates
8:20
Conclusion
本协议描述了如何制作从小鼠多能干细胞(PSC)功能性鼻窦淋巴组织。 T-BOX3(TBX3)表达以及心肌肌球蛋白重链(MYH6)启动抗生素的选择导致了高纯度的起搏细胞聚集。这些“诱导窦房结-机构”(“iSABs”)含有80%以上的起搏细胞,显示高度增加跳动的速度,并能步伐心肌体外 。
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