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DOI :
10.3791/52514-v
•
9:57 min
May 28th, 2015
Chapters
0:05
Title
1:28
Preparing the Host Embryo
3:30
Preparing the Donor Graft Tissue
5:05
Grafting the Tissue
6:11
Injecting Dil into Blood Vessels
7:20
Harvest Embryos for Sectioning or Wholemount Examination
8:05
Results: Dual Labeling in Chimeric Chick Embryos
9:19
Conclusion
ここでは、血管内DiI注射と組み合わせたひよこGFP 神経管の種内移植を用いた神経堤細胞および血管の二重標識を報告する。この実験技術により、臓器形成中にNCC由来の(腸管)神経系と血管系の発達を同時に可視化し、研究することができます。
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