Name: contrast imaging in mouse embryos using high-frequency ultrasound
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This work was supported by the Terry Fox Program of the National Cancer Institute of Canada.

OBS: De experimentella granskningsåtgärder som vidtas i denna studie godkändes av Animal Care kommittén vid Sunnybrook Research Institute (Toronto, Ontario, Kanada). Rutiner för human behandling av djur skall iakttas vid alla tillfällen. Det antas att utredaren är utbildad i den grundläggande hanteringen av ett ultraljud bildsystem. Detta protokoll fungerar bäst med två personer. 1. Djur Modeller <ol><li> Mate CD-1 manliga och kvinnliga Mus musculus att erhålla vildtyp ...

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Ultraljudskontrastmedel injicerades i slutskedet dräktighet musembryon och olinjära kontrastbilder förvärvades för att mäta perfusion parametrar och riktade mikrobubblor bindande. Framgångsrik avbildning av mikrobubblor i embryonal kärl var beroende av ett antal faktorer, den första är embryot livskraft. All utrustning och apparater framställdes i förväg för att minimera den tid som krävs för isolering av embryon från livmodern till början av injektionen. Eftersom effekterna av enstaka eller upprepad e...

Kontrastförstärkt ultraljud använder mikrobubblor kontrastmedel för att visualisera och karakterisera vaskulära miljön. Dessa medel möjliggör icke-invasiv bedömning av mikrocirkulationen, kärlteckning och kardiovaskulär funktion. Dessutom kan modifiering av bubblan ytan resultera i målinriktad mikrobubblor bindning till endotelceller biomarkörer, som visats i prekliniska applikationer av angiogenes, åderförkalkning och inflammation 1,2 gör molekylär ultraljud av vaskulära händelser möjliga. Kontrastförstärkt ultraljud kan därför användas för att identifiera de komplexa och skiftande miljöer som påverkar friska och sjuka kärltillstånd 3-5. Under det senaste antal år har intresset för nyttan av mikrobubblor avbildning utvidgas till den mångsidiga musembryo modell. Som en modell för utveckling däggdjur, förbättrar fysiologisk införandet av mikrobubblor i embryonala kärlstudie av utvecklingscirkulationssystemet (t.ex. blodflöde, hjärtminutvolym) och i fall av transgena och riktade muterade musmodeller av hjärtsjukdom 6,7, kan ge insikter i hur genetiska faktorer förändrar kardiovaskulär funktion. I själva verket, analyserar kvantitativ och kvalitativ 2D av embryonala hjärnkärl har redan uppnåtts 8. Dessutom presenterar musen embryot som en utmärkt modell för att undersöka bindningen av riktade mikrobubblor till vaskulära markörer in vivo. Et al. Bartelle 9, till exempel, har infört avidin mikrobubblor i embryohjärt ventriklarna att bedöma genom riktad bindning i Biotag-BirA transgena embryon och undersöka kärlanatomi. Den generation av heterozygota och homozygota musmodeller kan också användas som ett surrogat för tumörmodellstudier som syftar till att definiera kvantitativa natur molekylär ultraljud - ett viktigt riktmärke i att översätta denna teknik till kliniken. 8-10. I livmodern injektioner, dock står inför ett antal utmaningar. Dessa inkluderar injektion vägledning, behovet av att motverka rörelse i modern och exterioriserad embryo, underhåll av hemodynamisk livskraft hos modern och exterioriserades embryon, adresse långsiktiga effekter av narkos och komplikationer på grund av blödning 11. Därför var målet med undersökningen att utveckla en teknik för att injicera mikrobubblor i isolerade levande slutskedet embryon 12. Detta alternativ ger större frihet i fråga om insprutningsstyrning och positionering, reproducerbarhet av avbildningsplanet utan hinder, och förenklad bildanalys och kvantifiering. I den aktuella studien, vi beskriva ett nytt förfarande för injektion av mikrobubblor i levande murina embryon for att studera mikrobubblor kinetiska beteende och studera riktade mikrobubblor bindning till endogena endotelceller ytmarkörer. Icke-linjär kontrastspecifika ultraljud används för att mäta om ett antal grundläggande perfusion parametrar inklusive toppförstärkning (PE), tvätta-in hastighet och tid till maximal (TTP) i isolerade E17.5 embryon. Vi visar också giltigheten av embryot modell för bedömning av kvantitativ karaktär molekylär ultraljud i en embryonal endoglin funktionsförlust transgen musmodell där endoglin är en kliniskt relevant mål på grund av dess höga uttryck i vaskulära endotelceller på ställen där aktiv angiogenes 13 . Vidhäftningen av endoglin inriktade (MB E), råtta isotypkontroll IgG 2 kontroll (MB C) och oriktade (MB U) mikrobubblor utvärderas i heterozygot endoglin (Eng +/-) och homozygot endoglin (Eng + / +) uttrycker embryon. Analys av den målinriktade binding avslöjar att molekylär ultraljud är kapabel att skilja mellan endoglin genotyper och avser receptortäthet till kvantifierbara molekylära ultraljudsnivåer.

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			<td>This antibody/microbubble combination is often required as experimental control&#160;</td>
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		</tr>
		<tr height="19">
			<td height="19" style="height:19px;">Biotinylated rat MJ 7/18 antibody to mouse endoglin</td>
			<td style="width:215px;">In house hybridoma</td>
			<td style="width:207px;"></td>
			<td>Outside antibodies may also be appropriate: we&#160; have used eBioscience (13-1051-85 ) in the past</td>
		</tr>
		<tr height="19">
			<td height="19" style="height:19px;">Distilled water</td>
			<td style="width:215px;"></td>
			<td style="width:207px;"></td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Embryo media</td>
			<td style="width:215px;"></td>
			<td style="width:207px;"></td>
			<td></td>
		</tr>
		<tr height="19">
			<td height="19" style="height:19px;">&#160;&#160;&#160;&#160; 500 mL Dulbecco&#8217;s Modified Eagle&#8217;s Medium with high glucose</td>
			<td>Sigma</td>
			<td>D5796</td>
			<td></td>
		</tr>
		<tr height="19">
			<td height="19" style="height:19px;">&#160;&#160;&#160;&#160; 50 mL Fetal Bovine Serum</td>
			<td>ATCC</td>
			<td style="width:207px;">30-2020</td>
			<td>lot # 7592456</td>
		</tr>
		<tr height="19">
			<td height="19" style="height:19px;">&#160;&#160;&#160;&#160; Hepes&#160;</td>
			<td style="width:215px;">Gibco</td>
			<td style="width:207px;">15630</td>
			<td>5mL, 1M</td>
		</tr>
		<tr height="19">
			<td height="19" style="height:19px;">&#160;&#160;&#160;&#160; Penicillin-Streptomycin&#160;</td>
			<td style="width:215px;">Gibco</td>
			<td style="width:207px;">15140-122</td>
			<td>5 mL, 10,000 units Pen., 10,000 ug Strep</td>
		</tr>
		<tr height="19">
			<td height="19" style="height:19px;">Ethanol, 70%</td>
			<td style="width:215px;"></td>
			<td style="width:207px;"></td>
			<td></td>
		</tr>
		<tr height="19">
			<td height="19" style="height:19px;">Ice</td>
			<td style="width:215px;"></td>
			<td style="width:207px;"></td>
			<td></td>
		</tr>
		<tr height="19">
			<td height="19" style="height:19px;">Paraformaldehyde</td>
			<td style="width:215px;">Sigma</td>
			<td style="width:207px;">76240</td>
			<td>4%</td>
		</tr>
		<tr height="19">
			<td height="19" style="height:19px;">Phosphate Buffered Saline [1x]&#160;</td>
			<td style="width:215px;">Sigma</td>
			<td style="width:207px;">D8537</td>
			<td>1x, w/o calcium chloride &#38; magnesium chloride</td>
		</tr>
		<tr height="19">
			<td height="19" style="height:19px;">Pregnant mouse, CD-1</td>
			<td style="width:215px;">Charles River Laboratories Inc.&#160;</td>
			<td style="width:207px;"></td>
			<td></td>
		</tr>
		<tr height="19">
			<td height="19" style="height:19px;">0.9% sodium chloride (saline)</td>
			<td style="width:215px;">Hospira</td>
			<td style="width:207px;">0409-7984-11</td>
			<td></td>
		</tr>
		<tr height="19">
			<td height="19" style="height:19px;">Ultrasound contrast agent, target ready and untargeted</td>
			<td style="width:215px;">MicroMarker; VisualSonics Inc.</td>
			<td style="width:207px;"></td>
			<td></td>
		</tr>
		<tr height="19">
			<td height="19" style="height:19px;">Ultrasound gel (Aquasonic 100, colourless)</td>
			<td style="width:215px;">CSP Medical</td>
			<td style="width:207px;">133-1009</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;"></td>
			<td style="width:215px;"></td>
			<td style="width:207px;"></td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Equipment</td>
			<td style="width:215px;"></td>
			<td style="width:207px;"></td>
			<td></td>
		</tr>
		<tr height="19">
			<td height="19" style="height:19px;"></td>
			<td style="width:215px;"></td>
			<td style="width:207px;"></td>
			<td></td>
		</tr>
		<tr height="19">
			<td height="19" style="height:19px;">Cell culture plates (4) :&#160; 100x20 mm</td>
			<td style="width:215px;">Fisher Scientific</td>
			<td style="width:207px;">08-772-22</td>
			<td></td>
		</tr>
		<tr height="19">
			<td height="19" style="height:19px;">Cell culture plates (12) : 60x15 mm</td>
			<td style="width:215px;">Sigma</td>
			<td style="width:207px;">D8054</td>
			<td></td>
		</tr>
		<tr height="19">
			<td height="19" style="height:19px;width:419px;">Centrifuge</td>
			<td>Sorvall Legend RT centrifuge&#160;</td>
			<td style="width:207px;"></td>
			<td></td>
		</tr>
		<tr height="19">
			<td height="19" style="height:19px;">Conical tubes, 50 mL BD Falcon</td>
			<td style="width:215px;">VWR</td>
			<td style="width:207px;">21008-938</td>
			<td></td>
		</tr>
		<tr height="19">
			<td height="19" style="height:19px;">Diluent</td>
			<td style="width:215px;">Beckman Coulter</td>
			<td style="width:207px;">Isoton II Diluent, 8448011</td>
			<td></td>
		</tr>
		<tr height="19">
			<td height="19" style="height:19px;">Dissection scissors (Wagner)</td>
			<td style="width:215px;">Fine Science Tools</td>
			<td style="width:207px;">Wagner 14068-12</td>
			<td></td>
		</tr>
		<tr height="19">
			<td height="19" style="height:19px;">Forceps (2), Dumont SS (0.10x0.06 mm)</td>
			<td style="width:215px;">Fine Science Tools</td>
			<td style="width:207px;">11200-33</td>
			<td></td>
		</tr>
		<tr height="19">
			<td height="19" style="height:19px;">Forceps, splinter</td>
			<td style="width:215px;">VWR</td>
			<td style="width:207px;">25601-134</td>
			<td></td>
		</tr>
		<tr height="19">
			<td height="19" style="height:19px;">Glass beaker, 2 L (Griffin Beaker)</td>
			<td style="width:215px;">VWR</td>
			<td style="width:207px;">89000-216</td>
			<td></td>
		</tr>
		<tr height="19">
			<td height="19" style="height:19px;">Glass capillaries, 1x90 mm GD-1 with filament</td>
			<td style="width:215px;">Narishige</td>
			<td style="width:207px;">GD-1</td>
			<td></td>
		</tr>
		<tr height="19">
			<td height="19" style="height:19px;">Glass needle puller</td>
			<td style="width:215px;">Narishige</td>
			<td style="width:207px;">PN-30</td>
			<td></td>
		</tr>
		<tr height="19">
			<td height="19" style="height:19px;">Gloves</td>
			<td style="width:215px;">Ansell</td>
			<td style="width:207px;">4002</td>
			<td></td>
		</tr>
		<tr height="19">
			<td height="19" style="height:19px;">Gross anatomy probe</td>
			<td style="width:215px;">Fine Science Tools</td>
			<td style="width:207px;">10088-15</td>
			<td></td>
		</tr>
		<tr height="19">
			<td height="19" style="height:19px;">Hot plate</td>
			<td style="width:215px;">VWR</td>
			<td style="width:207px;">89090-994</td>
			<td></td>
		</tr>
		<tr height="19">
			<td height="19" style="height:19px;">Ice bucket</td>
			<td style="width:215px;">Cole Parmer</td>
			<td style="width:207px;">RK 06274-01</td>
			<td></td>
		</tr>
		<tr height="19">
			<td height="19" style="height:19px;">Imaging Platform</td>
			<td style="width:215px;">VisualSonics Inc.</td>
			<td style="width:207px;">Integrated Rail System</td>
			<td></td>
		</tr>
		<tr height="19">
			<td height="19" style="height:19px;">Light source, fiber-optic</td>
			<td style="width:215px;">Fisher Scientific</td>
			<td style="width:207px;">12-562-36</td>
			<td>Ideally has adjustable arms</td>
		</tr>
		<tr height="19">
			<td height="19" style="height:19px;">Luers (12), polypropylene barbed female &#188;-28 UNF thread</td>
			<td style="width:215px;">Cole Parmer</td>
			<td style="width:207px;">45500-30</td>
			<td></td>
		</tr>
		<tr height="19">
			<td height="19" style="height:19px;">Micro-ultrasound system, high-frequency</td>
			<td style="width:215px;">VisualSonics Inc.</td>
			<td style="width:207px;">Vevo2100</td>
			<td></td>
		</tr>
		<tr height="19">
			<td height="19" style="height:19px;">Needles, 21 gauge&#160; (1&#8221;)</td>
			<td style="width:215px;">VWR</td>
			<td style="width:207px;">305165</td>
			<td></td>
		</tr>
		<tr height="19">
			<td height="19" style="height:19px;">Particle size analyzer</td>
			<td style="width:215px;">Beckman Coulter</td>
			<td style="width:207px;">Multisizer 3 Coulter Counter</td>
			<td></td>
		</tr>
		<tr height="19">
			<td height="19" style="height:19px;">Perforated spoon (Moria)</td>
			<td style="width:215px;">Fine Science Tools</td>
			<td style="width:207px;">MC 17 10373-17</td>
			<td></td>
		</tr>
		<tr height="19">
			<td height="19" style="height:19px;">Pins (6), black anodized minutien 0.15 mm</td>
			<td style="width:215px;">Fine Science Tools</td>
			<td style="width:207px;">26002-15</td>
			<td></td>
		</tr>
		<tr height="57">
			<td height="57" style="height:57px;">Pipettors [2-20 uL, 20-200uL, 100-1000uL]</td>
			<td style="width:215px;">Eppendorf</td>
			<td style="width:207px;">Research Plus&#160; adjustable 3120000038;&#160;&#160;&#160;&#160;&#160;&#160; 3120000054;&#160;&#160;&#160;&#160;&#160;&#160; 3120000062</td>
			<td style="width:639px;"></td>
		</tr>
		<tr height="38">
			<td height="38" style="height:38px;">Pipettor tips [2-200uL, 50-1000uL]</td>
			<td style="width:215px;">Eppendorf</td>
			<td style="width:207px;">epT.I.P.S.&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160; 22491334;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160; 022491351</td>
			<td style="width:639px;"></td>
		</tr>
		<tr height="19">
			<td height="19" style="height:19px;">Scissors</td>
			<td style="width:215px;"></td>
			<td style="width:207px;"></td>
			<td></td>
		</tr>
		<tr height="19">
			<td height="19" style="height:19px;">Sylgard 184 Silicone Elastomer Kit</td>
			<td style="width:215px;">Dow Corning</td>
			<td style="width:207px;"></td>
			<td></td>
		</tr>
		<tr height="19">
			<td height="19" style="height:19px;">Tubing, Tygon laboratory 1/32x3/32&#8221;</td>
			<td style="width:215px;">VWR</td>
			<td style="width:207px;">63010-007</td>
			<td></td>
		</tr>
		<tr height="19">
			<td height="19" style="height:19px;">Wooden applicator stick (swab, cotton head)</td>
			<td style="width:215px;">VWR</td>
			<td style="width:207px;">CA89031-270</td>
			<td></td>
		</tr>
		<tr height="19">
			<td height="19" style="height:19px;">Surgical microscope 5-8x magnification</td>
			<td style="width:215px;">Fisher Scientific</td>
			<td style="width:207px;">Steromaster</td>
			<td></td>
		</tr>
		<tr height="19">
			<td height="19" style="height:19px;">Syringes, 1 mL Normject</td>
			<td style="width:215px;">Fisher</td>
			<td style="width:207px;">14-817-25</td>
			<td></td>
		</tr>
		<tr height="19">
			<td height="19" style="height:19px;">Syringes (10), 30 mL</td>
			<td style="width:215px;">VWR</td>
			<td style="width:207px;">CA64000-041</td>
			<td></td>
		</tr>
		<tr height="19">
			<td height="19" style="height:19px;">Syringe infusion pump&#160;</td>
			<td style="width:215px;">Bio-lynx&#160;</td>
			<td style="width:207px;">NE-1000</td>
			<td></td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;">Thermometer, -20-110oC</td>
			<td style="width:215px;">VWR</td>
			<td style="width:207px;">89095-598</td>
			<td></td>
		</tr>
		<tr height="19">
			<td height="19" style="height:19px;">Timer</td>
			<td style="width:215px;">VWR</td>
			<td style="width:207px;">33501-418</td>
			<td></td>
		</tr>
		<tr height="19">
			<td height="19" style="height:19px;">Tubes, Eppendorf</td>
			<td style="width:215px;">VWR</td>
			<td style="width:207px;">20170-577</td>
			<td></td>
		</tr>
		<tr height="19">
			<td height="19" style="height:19px;">Tube racks (3)</td>
			<td style="width:215px;">VWR</td>
			<td style="width:207px;">82024-462</td>
			<td></td>
		</tr>
		<tr height="19">
			<td height="19" style="height:19px;">Ultrasound transducer, 20 MHz</td>
			<td style="width:215px;">VisualSonics Inc.</td>
			<td style="width:207px;">MS250</td>
			<td></td>
		</tr>
		<tr height="19">
			<td height="19" style="height:19px;">Vannas-Tubingen, angled up</td>
			<td style="width:215px;">Fine Science Tools</td>
			<td style="width:207px;">15005-08</td>
			<td></td>
		</tr>
	</tbody>
</table>

contrast imaging in mouse embryos using high-frequency ultrasound

Ultrasound contrast-enhanced imaging can convey essential quantitative information regarding tissue vascularity and perfusion and, in targeted applications, facilitate the detection and measure of vascular biomarkers at the molecular level. Within the mouse embryo, this noninvasive technique may be used to uncover basic mechanisms underlying vascular development in the early mouse circulatory system and in genetic models of cardiovascular disease. The mouse embryo also presents as an excellent model for studying the adhesion of microbubbles to angiogenic targets (including vascular endothelial growth factor receptor 2 (VEGFR2) or &#945;v&#946;3) and for assessing the quantitative nature of molecular ultrasound. We therefore developed a method to introduce ultrasound contrast agents into the vasculature of living, isolated embryos. This allows freedom in terms of injection control and positioning, reproducibility of the imaging plane without obstruction and motion, and simplified image analysis and quantification. Late gestational stage (embryonic day (E)16.6 and E17.5) murine embryos were isolated from the uterus, gently exteriorized from the yolk sac and microbubble contrast agents were injected into veins accessible on the chorionic surface of the placental disc. Nonlinear contrast ultrasound imaging was then employed to collect a number of basic perfusion parameters (peak enhancement, wash-in rate and time to peak) and quantify targeted microbubble binding in an endoglin mouse model. We show the successful circulation of microbubbles within living embryos and the utility of this approach in characterizing embryonic vasculature and microbubble behavior.

Injektion av ultraljudskontrastmedel till ex utero musembryon är beroende av framgångsrika isolering av levande, sen graviditetsscen embryon från livmodern och underhåll av lönsamheten under loppet av injektionen och tillhörande ultraljudsundersökningar. När fostret har exterioriseras och placeras, såsom visas i fig 1, är det möjligt noggrann injicering av kontrastmedel in i embryonala vaskulaturen. En typisk B-mode ultraljudsbild av en E17.5 musembryo visas i fig 2A.</strong...

Watch this Scientific Journal Video about Contrast Imaging in Mouse Embryos Using High-frequency Ultrasound at JoVE.com

Contrast Imaging in Mouse Embryos Using High-frequency Ultrasound

Här presenterar vi ett protokoll för att injicera ultraljudsmikrobubblor kontrastmedel i levande, isolerade sena dräktighetsstadiet murina embryon. Denna metod gör det möjligt att studera perfusion parametrar och kärl molekylära markörer inom embryot använder kontrastförstärkt högfrekvent ultraljud.

Contrast Imaging i musembryon Använda Högfrekvent Ultraljud

Ultrasound contrast-enhanced imaging can convey essential quantitative information regarding tissue vascularity and perfusion and, in targeted ...

Research

JoVE Journal

Developmental Biology

Analyzing In Vivo Cell Migration using Cell Transplantations and Time-lapse Imaging in Zebrafish Embryos

Analyzing In Vivo Cell Migration using Cell Transplantations and Time-lapse Imaging in Zebrafish Embryos

analysera<em&gt; In Vivo</em&gt; Cell Migration använder Cell Transplantationer och Time-lapse avbildning i Zebrafish embryon

Cell migration is key to many physiological and pathological conditions, including cancer metastasis. The cellular and molecular bases of cell migration ...

Live Imaging of Mouse Secondary Palate Fusion

The fusion of the secondary palatal shelves to form the intact secondary palate is a key process in mammalian development and its disruption can lead to ...

A Novel Use of Three-dimensional High-frequency Ultrasonography for Early Pregnancy Characterization in the Mouse

En ny användning av tredimensionella högfrekvent ultraljud för tidig graviditet karakterisering i musen

High-frequency ultrasonography (HFUS) is a common method to non-invasively monitor the real-time development of the human fetus in utero. The mouse is ...

Fetal Mouse Cardiovascular Imaging Using a High-frequency Ultrasound (30/45MHZ) System

Fetal Mouse Cardiovascular Imaging Using a High-frequency Ultrasound 30/45MHZ System

Fostrets mus hjärt Imaging använder ett högfrekvent ultraljud (30 / 45MHZ)

Congenital heart defects (CHDs) are the most common cause of childhood morbidity and early mortality. Prenatal detection&#160;of the underlying molecular ...

Visualizing the Node and Notochordal Plate In Gastrulating Mouse Embryos Using Scanning Electron Microscopy and Whole Mount Immunofluorescence

Visualisera den noden och Notochordal plattan i Gastrulating musembryon som använder Scanning Electron Microscopy och hela Mount immunofluorescens

The post-implantation mouse embryo undergoes major shape changes after the initiation of gastrulation and morphogenesis. A hallmark of morphogenesis is ...

High Frequency Ultrasound for the Analysis of Fetal and Placental Development In Vivo

High Frequency Ultrasound for the Analysis of Fetal and Placental Development In Vivo

Hög frekvens ultraljud för analys av foster och placenta utveckling In Vivo

Ultrasound imaging is a widespread method used to detect organ anomalies and tumors in human and animal tissues. The method is non-invasive, harmless, and ...

Analysis of Congenital Heart Defects in Mouse Embryos Using Qualitative and Quantitative Histological Methods

Analys av medfödda hjärtfel i musembryon med hjälp av kvalitativa och kvantitativa histologiska metoder

Congenital heart defects (CHD) are the most common type of birth defect in humans, affecting up to 1% of all live births. However, the underlying causes ...

High-Frequency Ultrasound Echocardiography to Assess Zebrafish Cardiac Function

Högfrekvent ultraljud ekokardiografi för att bedöma zebrafisk hjärtfunktion

The zebrafish (Danio rerio) has become a very popular model organism in cardiovascular research, including human cardiac diseases, largely due to its ...

Imaging Intranuclear Actin Rods in Live Heat Stressed Drosophila Embryos

Imaging Intranuclear Actin Rods in Live Heat Stressed Drosophila Embryos

Imaging Intranuclear Actin Rods i levande värme stressade Drosophila embryon

The purpose of this protocol is to visualize intranuclear actin rods that assemble in live Drosophila melanogaster embryos following heat stress. Actin ...

Is My Mouse Pregnant? High-Frequency Ultrasound Assessment

Är musen gravid? Ultraljudsbedömning med hög frekvens

The mouse is the mammalian animal model of choice for many human diseases and biological processes. Developmental biology often requires staged-pregnant ...