Name: a murine model of arterial restenosis: technical aspects of femoral wire injury
Uploaded: 2015-03-10T15:00:00
Description: Watch this Scientific Journal Video about A Murine Model of Arterial Restenosis: Technical Aspects of Femoral Wire Injury at JoVE.com

This work was supported by a Wisconsin Partnership Program New Investigator Award (ID 2832), a National Heart, Lung, Blood Institute R01 Grant (HL-068673) and a T32 training Grant (HL-110853). We thank Dr. Melina Kibbe&#8217;s group at Northwestern University for providing helpful information.

NOT: Etik Açıklama: Tüm işlemler Wisconsin Üniversitesi Kurumsal Hayvan Bakım ve Kullanım Kurulu tarafından onaylanan Laboratuvar Hayvanları Bakım ve Kullanımı Kılavuzu (Ulusal Akademiler Press, 8. baskı, 2011), ve protokollere uygun. Tüm ameliyatlar (oran 2 ml / dak akış, teneffüs yoluyla) izofluran anestezi altında, ve tüm çabalar acı en aza indirmek için yapılmıştır. Hayvanlar kademeli CO2 ile doldurulmuş bir odacığm içine kurban edilmiştir. Genel ...

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	<li>Dzau, V. J., Braun-Dullaeus, R. C., Sedding, D. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Vascular+proliferation+and+atherosclerosis:+new+perspectives+and+therapeutic+strategies.">Vascular proliferation and atherosclerosis: new perspectives and therapeutic strategies.</a> Nat Med. 8 (11), 1249-1256 (2002).</li><li>Hui, D. Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Intimal+hyperplasia+in+murine+models.">Intimal hyperplasia in murine models.</a> Curr Drug Targets. 9 (3), 251-260 (2008).</li><li>Ferns, G. A., Avades, T. Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+mechanisms+of+coronary+restenosis:+insights+from+experimental+models.">The mechanisms of coronary restenosis: insights from experimental models.</a> Int J Exp Pathol. 81 (2), 63-88 (2000).</li><li>Sata, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+mouse+model+of+vascular+injury+that+induces+rapid+onset+of+medial+cell+apoptosis+followed+by+reproducible+neointimal+hyperplasia.">A mouse model of vascular injury that induces rapid onset of medial cell apoptosis followed by reproducible neointimal hyperplasia.</a> J Mol Cell Cardiol. 32 (11), 2097-2104 (2000).</li><li>Xu, Q. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mouse+models+of+arteriosclerosis:+from+arterial+injuries+to+vascular+grafts.">Mouse models of arteriosclerosis: from arterial injuries to vascular grafts.</a> Am J Pathol. 165 (1), 1-10 (2004).</li><li>Lv, L., Meng, Q., Ye, M., Wang, P., Xue, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=STAT4+deficiency+protects+against+neointima+formation+following+arterial+injury+in+mice.">STAT4 deficiency protects against neointima formation following arterial injury in mice.</a> J Mol Cell Cardiol. 74C, 284-294 (2014).</li><li>Zhu, B., Kuhel, D. G., Witte, D. P., Hui, D. Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Apolipoprotein+E+inhibits+neointimal+hyperplasia+after+arterial+injury+in+mice.">Apolipoprotein E inhibits neointimal hyperplasia after arterial injury in mice.</a> Am J Pathol. 157 (6), 1839-1848 (2000).</li><li>Fu, Y., Duru, E. A., Davies, M. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effect+of+metabolic+syndrome+on+the+response+to+arterial+injury.">Effect of metabolic syndrome on the response to arterial injury.</a> J Surg Res. , (2014).</li><li>Kuhel, D. G., Zhu, B., Witte, D. P., Hui, D. Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Distinction+in+genetic+determinants+for+injury-induced+neointimal+hyperplasia+and+diet-induced+atherosclerosis+in+inbred+mice.">Distinction in genetic determinants for injury-induced neointimal hyperplasia and diet-induced atherosclerosis in inbred mice.</a> Arterioscler Thromb Vasc Biol. 22 (6), 955-960 (2002).</li><li>Cooley, B. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mouse+strain+differential+neointimal+response+in+vein+grafts+and+wire-injured+arteries.">Mouse strain differential neointimal response in vein grafts and wire-injured arteries.</a> Circ J. 71 (10), 1649-1652 (2007).</li><li>Ishigami, N., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Deficiency+of+CuZn+superoxide+dismutase+promotes+inflammation+and+alters+medial+structure+following+vascular+injury.">Deficiency of CuZn superoxide dismutase promotes inflammation and alters medial structure following vascular injury.</a> J Atheroscler Thromb. 18 (11), 1009-1017 (2011).</li></ol>

tel yaralanma prosedürü, biz 12-16 haftalık bir yaş erkek C57BL / 6 fareler kullanıldı sürece onların anatomik yapıları benzer olarak. 4 Bu yazıda farelerin tüm suşları için geçerlidir. Bizim ön çalışmalardan öğrendiğimiz gibi, 10 haftadan küçük farelerin femoral arter genellikle tel ekleme oldukça zorlu olduğunu çok küçük. Diğer yandan, 16 haftadan daha yaşlı farelerin tel ekleme teknik olarak daha kolay olan, ancak çok büyük bir çeşitlilik neointimal hiperplazi neden ...

In the era of expanded application of endovascular treatments for various cardiovascular diseases, restenosis after angioplasty is one of the major problems for patients undergoing such treatments. Damage to the vascular endothelium at the time of angioplasty, in concert with the atherosclerotic background, induces excessive smooth muscle cell proliferation in the medial layer, resulting in neointimal hyperplasia.1,2 A viable animal model that recapitulates post-angioplasty neointimal hyperplasia is, thus, important not only for the investigation of disease mechanisms but also for the development of effective therapeutics to treat this pathology.
Mice represent an excellent model animal to recapitulate neointimal hyperplasia for the following reasons: the genetic backgrounds of experimental mice are well established; a wide variety of genetically-modified strains are available;2 obtaining littermates of the same background is easy; and the cost of the animals is relatively low. Arterial ligation model and wire injury model are the two most common mouse models of mechanically-induced neointimal hyperplasia. The arterial ligation model is easy to create, but physiologically dissimilar to the actual angioplasty procedure. The wire injury model closely mimics actual angioplasty procedures but is technically difficult due to the small size of mouse arteries.2,3 Sata et al. first described a wire injury method for mouse femoral arteries based on the anatomical structure of the vasculature and the use of proper-sized flexible wire. Utilizing this technique, they succeeded in reproducibly inducing neointimal hyperplasia in various strains of mice.4
Although femoral wire injury is a well-established model, some of the technical aspects of the technique are highly challenging compared to other models such as ligation.5 The purpose of this paper is to describe our mouse wire injury model procedures in detail, which is a modified version of Sata&#8217;s original method. We have made two main modifications: 1) Looping only the arteries, and 2) No lidocaine use.

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a murine model of arterial restenosis: technical aspects of femoral wire injury

Ateroskleroz neden Kardiyovasküler hastalık gelişmiş dünyada önde gelen ölüm nedenidir. Nedeniyle aterosklerotik plak gelişimi veya kurulmuş plakların çatlaması, damar lümeninin daralması gibi miyokard enfarktüsü ve inme gibi çeşitli hastalıklar giden normal kan akışını keser. Anjiyoplasti gibi klinik endovasküler işlemlerde yaygın lümen yeniden açmak için yapılır. Bununla birlikte, bu tedaviler, kaçınılmaz olarak bir fazla iyileşme yanıtı ve damar restenozu (yeniden daralma) neden olan lümen içine uzanan bir neointimal plak gelişimini tetikleme, damar duvarı hem de vasküler endotel hasar. Restenoz ateroskleroz için endovasküler tedavi başarısızlığı önemli bir nedenidir. Böylece, restenoz preklinik hayvan modelleri öteleme vasküler müdahalelere yaklaşımları yanı sıra patofizyolojik mekanizmaları araştırmak için hayati önem taşımaktadır. Bir çok murin experime arasındayakından endotel ve damar duvarını hem yaralı anjiyoplasti işlemi benzer çünkü ntal modelleri, femoral arter tel yaralanması yaygın anjiyoplasti sonrası restenoz çalışmaları için en uygun olarak kabul edilir. Ancak, birçok araştırmacı nedeniyle teknik zorluk yüksek derecede bu modeli kullanan zorluk. Bu, bir metal tel belirgin neointima indüklemek için yeterli darbe üretmek için, yaklaşık olarak tel üç kat daha incedir femoral arter içine takılması gerekir öncelikle çünkü. Burada, biz etkili bu modelin önemli teknik zorlukların üstesinden gelmek için gerekli cerrahi ayrıntıları açıklar. , Sunulan prosedürleri takip gerçekleştirerek fare femoral arter tel yaralanması kolaylaşır. Aşina sonra, bütün işlem 20 dakika içinde tamamlanabilir.

Ameliyatı takiben dört hafta, tel yaralı femoral arter hasat ve neointima oluşumunu analiz etmek için bir parafin blok örneği olun. Yukarıda tarif edilen genel anestezi neden. Hemen göğüs açın ve sol ventrikül bir 20-gauge kelebek iğne takın. Bir infüzyon şişe hattını bağlayın. % 4 paraformaldehid (genellikle 10-20 ml, her biri), ardından PBS damla infüzyon yoluyla perfüzyon fiksasyon gerçekleştirin. Hayvan daha sonra tel yaralı CFA çıkar ve 4 ° C&#39;de gece boyunca% 4 paraforma...

Watch this Scientific Journal Video about A Murine Model of Arterial Restenosis: Technical Aspects of Femoral Wire Injury at JoVE.com

A Murine Model of Arterial Restenosis: Technical Aspects of Femoral Wire Injury

restenoz fare femoral arter yaralanması tel modeli teknik olarak zordur. Bu protokolde başarıyla restenoz çalışmaları için tutarlı neointima ikna etmek tel yaralanması gerçekleştirmek için anahtar teknik detaylar önemlidir göstermektedir.

Arter Restenozun bir fare modeli: Femur Tel Yaralanma Teknik Yönleri

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