Sign In

JoVE Journal

Bioengineering

A subscription to JoVE is required to view this content.

live

Speed

×

MEDIA_ELEMENT_ERROR: Format error

Novel Atomic Force Microscopy Based Biopanning for Isolation of Morphology Specific Reagents against TDP-43 Variants in Amyotrophic Lateral Sclerosis

8.7K Views

13:31 min

February 12th, 2015

February 12th, 2015

8,700 Views

0:05

Title

2:47

12 Rounds of Negative Panning Against Bovine Serum Albumin (BSA)

4:19

AFM Process

6:26

8 Rounds of Negative Panning Against Healthy Immunoprecipitated TDP-43

7:54

Positive Panning against Immunoprecipitated TDP-43 from Individuals with ALS

11:17

Results: Analysis of Potential ALS Clones

12:59

Conclusion

Using atomic force microscopy in combination with biopanning technology we created a negative and positive biopanning system to acquire antibodies against disease-specific protein variants present in any biological material, even at low concentrations. We were successful in obtaining antibodies to TDP-43 protein variants involved in Amyotrophic Lateral Sclerosis.

We use cookies to enhance your experience on our website.

By continuing to use our website or clicking “Continue”, you are agreeing to accept our cookies.

Learn More