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본래 Neuroepithelium에서 마우스 강한 감각 뉴런의 천공 패치 클램프 녹음 : 확인 된 부 취제 수용체를 발현 뉴런의 기능 분석

DOI :

10.3791/52652-v

July 13th, 2015

July 13th, 2015

26,445 Views

1UMR Centre des Sciences du Goŭt et de l'Alimentation, CNRS, INRA, Université de Bourgogne

Analyzing the physiological properties of olfactory sensory neurons still faces technical limitations. Here we record them through perforated patch-clamp in an intact preparation of the olfactory epithelium in gene-targeted mice. This technique allows the characterization of membrane properties and responses to specific ligands of neurons expressing defined olfactory receptors.

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