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DOI :
10.3791/52665-v
May 1st, 2015
Chapters
0:05
Title
2:09
Cell Culture and Lentiviral Transfection
3:20
3D-Multiple Myeloma Spheroid Model
4:47
3D Multiple Myeloma Xenograft Model in the CAM
6:17
Quantification of eGFP Protein by ELISA
7:03
Immunohistochemical Analysis of Blood Vessels and Invading Tumor Cells
8:05
Results: Bortezomib Treatment of Multiple Meyloma Xenografts
9:17
Conclusion
ヒト多発性骨髄腫(MM)細胞は、間葉系細胞と生存および増殖のための細胞外マトリックス成分の支援微小環境が必要です。我々は、腫瘍の増殖、浸潤および血管形成に対する抗がん剤の効果を研究するために移植されたヒト骨髄腫および間葉細胞とin vivoでニワトリ胚モデルを確立しました。
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