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القيطم المورق نموذجا لتحديد الترجمة انخفاض قيمة

10.6K Views

10:24 min

September 27th, 2015

September 27th, 2015

10,627 Views

0:05

Title

2:39

mRNA Synthesis

3:55

Microinjection of Synthesized RNA and Stimulation of Oocyte Maturation

5:15

Germinal Vesicle Breakdown and Western Blot Analysis

6:21

Polyadenylation Assay

8:08

Results: Xenopus Oocyte Translation Analysis on Maturation

9:43

Conclusion

Protein synthesis control occurs mainly at the translation initiation step, deficiencies in which are linked to diverse disorders. To better understand their etiology, we described here a protocol using Xenopus laevis oocytes assessing the translation of mos transcript in the presence of a mutant of translation initiation factor eIF4G1.

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