INSERM UMR-S1172, CHRU Lille, University of Lille 1 and Lille 2

1 ARTICLES PUBLISHED IN JoVE

Biology

Xenopus laevis as a Model to Identify Translation Impairment

Amélie de Broucker¹, Pierre Semaille¹, Katia Cailliau², Alain Martoriati², Thomas Comptdaer¹, Jean-François Bodart², Alain Destée¹, Marie-Christine Chartier-Harlin¹

¹Team "Early Stages of Parkinson's Disease" of the Jean-Pierre Aubert Research Center, INSERM UMR-S1172, CHRU Lille, University of Lille 1 and Lille 2, ²Team "Signal Division Regulation", CNRS UMR 8576, University of Lille 1

Protein synthesis control occurs mainly at the translation initiation step, deficiencies in which are linked to diverse disorders. To better understand their etiology, we described here a protocol using Xenopus laevis oocytes assessing the translation of mos transcript in the presence of a mutant of translation initiation factor eIF4G1.