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Live Imaging of Nicotine Induced Calcium Signaling and Neurotransmitter Release Along Ventral Hippocampal Axons

DOI :

10.3791/52730-v

June 24th, 2015

June 24th, 2015

8,766 Views

1Department of Neurobiology and Behavior, Stony Brook University, 2Department of Pharmacological Science, Stony Brook University

We developed a gene-chimeric preparation of ventral hippocampal – accumbens circuit in vitro that allows direct live imaging to analyze presynaptic mechanisms of nicotinic acetylcholine receptors (nAChRs) mediated synaptic transmission. This preparation also provides an informative approach to study the pre- and post-synaptic mechanisms of synaptic plasticity.

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Nicotine

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