Name: athymic rat model for evaluation of engineered anterior cruciate ligament grafts
Uploaded: 2015-03-26T15:00:00
Description: Watch this Scientific Journal Video about Athymic Rat Model for Evaluation of Engineered Anterior Cruciate Ligament Grafts at JoVE.com

저자는이 프로젝트의 이전 반복에 기술적 공헌 가브리엘 AROM 마이클 Yeranosian에게 감사의 말씀을 전합니다. 이 프로젝트는 OREF 임상 과학자 교육 그랜트 (NL)에 의해 투자되었다, HH 리 외과 연구비 (NL), 재향 군인의 관리 BLR &amp; D 탁월성 평가 1 I01 BX00012601 (DM) 및 근골격계 이식 재단 젊은 연구자 상 (FP).

참고 : 모든 동물의 수술이 실험을 시작하기 전에 지역 수의학 직원과 동물 사용위원회에 의해 승인되었다. 전기 방사 된 폴리 카프로 락톤 비계의 1. 준비 <ol><li> w 10 %를 생성하는 1,1,1,3,3,3- 헥사 플루오로 -2- 프로판올의 과립 형태로 의료용 폴리 에스테르 말단 (ε 카프로 락톤)를 계량하고 용해 PCL 중합체 용액 w /. 균일 한 솔루션을 보장하기 위해 적어도 3 시간 동안 교반 ?...

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ACL 부상은 현 시점에서 재구성에 대한 제한 옵션 정형 외과 스포츠 수술의 일반적인 조건입니다. 생체 내에서 재생을 허용 ACL에 대한 적절한 대체 조직 공학을 개발하기 위해, 적절한 동물 모델이 요구된다. 흉선 쥐에서 전방 십자 인대 재건술의 재현 모델을 사용하여 생체 내 주입 같이 본 연구에서는 생분해 설계 이식의 제조는 설명한다. 이 모델은 세포 이식하고 통합하는 성장 인...

전방 십자 인대 (ACL) 파열은 무릎 하나의 가장 일반적인 인대 부상 중 하나입니다. 파열 ACL의 수술 개입없이 치유 할 수없는 때문에, 일상 생활뿐만 아니라 스포츠에 참여의 활동에 제한은 매년 억 달러 (3)의 추정 비용으로, 매년 2 수술을 175,000 명 이상의 환자를 구동한다. 현재, 이식 또는 동종 이식 건 중 하나는 인대 재건술에 사용됩니다. 높은 성공률 모두 이식과 동종 이식 교체로 달성 될 수 있지만, 심각한 합병증이 복원 옵션 4와 연결되어 있습니다. 이식 조직은 특히 재 파열 또는 멀티 인대 손상의 경우에, 공여부의 이환율과 관련된 공급에 제한됩니다. 한편, 동종 이식 조직 지연된 그래프트 통합 불리한 염증 반응, 이론적 감염성 위험 및 제한 좌제와 링크LY 5. 합성 비 분해성 이식은 1970 년대와 1980 년대에 개발되었지만 조기 이식 파열, 이물 반응, 골 용해 및 활액막염 (6)에 의해 방해되었다. 이러한 심각한 문제의 결과로서, 미국에서의 임상 사용할 수있는 합성 이식은 현재 존재하지 않는다. 때문에 기존의 이식 옵션 및 생물학, 공학, 재생 의학의 최근의 발전에 이러한 제한, ACL 이식을위한 조직 설계 솔루션에 큰 관심이 있었다. 현재 조직 공학 전략 영구 합성 재료 주입 7과 관련된 제한을 회피하면서 호스트 조직 내 증식을 허용하는 생물 분해성 및 합성 물질을 사용한다. 폴리 카프로 락톤 (PCL)는 FDA가 접착 장벽을 포함한 의료 분야의 다수의 승인과 8 드레싱 감겨 생분해 성 고분자, 즉 U를했습니다혈관, 뼈, 연골, 신경, 피부, 식도 조직 공학 5,9-16 포함한 다양한 애플리케이션에 나오지. 바람직한 생체 적합성, 상대적으로 긴 생체 내 반감기, 충분한 기계적 강도, 높은 탄성 조직 공학에서의이 중합체의 인기에 기여한다. 상처 치유의 쥐 모델에서 이식 전기 방사 PCL은 비 면역 원성 및 이상 반응 (13)없이 지방 조직으로 통합하는 것으로 나타났다. 전기 방사 된 PCL의 SEM 이미지는도 1에 도시된다. 크고 작은 동물 모델에서 현재 FDA 규제 기준, 유효성과 안전성으로 미국에서 임상 시험에 이동 PCL 또는 기타 설계 ACL 이식을 위해 필요한 것입니다. 또한, 생체 내 조건은 종종 시험관 조직 설계 ACL 이식의 특성을 보강 할 수 있습니다. 굴곡 digitor와자가 전방 십자 인대 재건술의 쥐 모델음 longus 건은 이전에 기본 ACL이 절단 대퇴 및 경골 터널을 뚫고하고, 이식이 통과하고 봉합 17-22로 제 위치에 고정 된 한에서, 기술되었다. 이 논문에서는 설계 ACL 교체의 평가보다는 이식 기반 재건 (그림 2)이 모델의 변형 예를 설명한다. 많은 동물 모델은 인대 조직 공학을 위해 존재하지만, 래트는 여러 이유로 큰 모델에 비해 유리하다. 이러한 장점은 쉽게 사육 및 처리, 적은 수의 윤리적 고려 사항 및 비용 절감의 17, 23 등이 있습니다. 또한, 쥐 모델 연골, 힘줄, 뼈 조직 공학 (24)를 포함하여 정형 조직 재생을위한 모델로서 광범위하게 사용되어왔다. 특히, 흉선 누드 쥐 최종 주입 O를 허용, 세포 매개 성 면역 반응 (25)의 부족으로 인해 선택되었다이 모델 F 이종 도너 세포는 또한 미래의 설계 그래프트 향상시킬. 이 방법의 논문에서는 전방 십자 인대 재건술의 흉선 쥐 모델의 제조 및 무 세포의 수술 주입, 생분해 성 고분자 이식에 대해 설명합니다.

<table border="0" cellpadding="0" cellspacing="0" width="888">
	<tbody>
		<tr height="34">
			<td height="34" style="height:34px;width:347px;">Name of Material/ Equipment</td>
			<td style="width:147px;">Company</td>
			<td style="width:77px;">Catalog Number</td>
			<td style="width:317px;">Comments/Description</td>
		</tr>
		<tr height="34">
			<td height="34" style="height:34px;width:347px;">Medical grade ester terminated poly (&#949;-caprolactone), granule form (MW = 110,000)</td>
			<td style="width:147px;">Lactel Absorbable Polymers</td>
			<td style="width:77px;"></td>
			<td style="width:317px;">Custom synthesized polymer to desired molecular weight</td>
		</tr>
		<tr height="17">
			<td height="17" style="height:17px;width:347px;">1,1,1,3,3,3-hexafluoro-2-propanol</td>
			<td style="width:147px;">Sigma-Aldrich</td>
			<td>105228</td>
			<td style="width:317px;">Solvent for PCL polymer</td>
		</tr>
		<tr height="17">
			<td height="17" style="height:17px;width:347px;">18G x 1 1/2&#34;bevel needle</td>
			<td style="width:147px;">BD Medical</td>
			<td>305196</td>
			<td style="width:317px;"></td>
		</tr>
		<tr height="17">
			<td height="17" style="height:17px;width:347px;">Remote Infuse/Withdraw Programmable Syringe Pump</td>
			<td style="width:147px;">Harvard Apparatus</td>
			<td style="width:77px;">702101</td>
			<td style="width:317px;"></td>
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			<td height="17" style="height:17px;width:347px;">VersaLaser VLS2.30 Laser Engraver</td>
			<td style="width:147px;">Microgeo USA</td>
			<td style="width:77px;">VLS2.30</td>
			<td style="width:317px;"></td>
		</tr>
		<tr height="34">
			<td height="34" style="height:34px;width:347px;">Expanded Plasma Cleaner 115V</td>
			<td style="width:147px;">Harrick Plasma</td>
			<td style="width:77px;">PDC-001</td>
			<td style="width:317px;">Plasma etch just prior to collagen coating for surface modification</td>
		</tr>
		<tr height="34">
			<td height="34" style="height:34px;width:347px;">PureCol&#160; Collagen Standard Solution, 3 mg/ml</td>
			<td style="width:147px;">Advanced Biomatrix</td>
			<td style="width:77px;">5015-A</td>
			<td style="width:317px;">Mix 8:1:2.5 solution of PureCol, 10x PBS, 0.1N NaOH 1:9 in 1x PBS</td>
		</tr>
		<tr height="17">
			<td height="17" style="height:17px;width:347px;">Suture, 5-0 Vicryl</td>
			<td style="width:147px;">Henry Schein</td>
			<td style="width:77px;">1086471</td>
			<td style="width:317px;"></td>
		</tr>
		<tr height="17">
			<td height="17" style="height:17px;">Suture, 4-0 Vicryl</td>
			<td style="width:147px;">Henry Schein</td>
			<td style="width:77px;">6540072</td>
			<td style="width:317px;"></td>
		</tr>
		<tr height="17">
			<td height="17" style="height:17px;width:347px;">Sharp-pointed Dissecting Scissors (Straight; 4.5 inch)</td>
			<td style="width:147px;">Fisher Scientific</td>
			<td style="width:77px;">8940</td>
			<td style="width:317px;"></td>
		</tr>
		<tr height="34">
			<td height="34" style="height:34px;width:347px;">Buphrenorphine hydrochloride</td>
			<td style="width:147px;">Sigma-Aldrich</td>
			<td style="width:77px;">B9275</td>
			<td style="width:317px;">Use 0.03 mg/kg for both intra- and post-operatively for pain control</td>
		</tr>
		<tr height="17">
			<td height="17" style="height:17px;width:347px;">Ampicillin, injectable</td>
			<td style="width:147px;">Henry Schein</td>
			<td style="width:77px;">1185678</td>
			<td style="width:317px;">Use 25 mg/kg subcutaneously during the procedure</td>
		</tr>
		<tr height="17">
			<td height="17" style="height:17px;width:347px;">K-wire, 1.6 mm</td>
			<td style="width:147px;">Spectrum Surgical</td>
			<td style="width:77px;">SI040062</td>
			<td style="width:317px;"></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:347px;">Keith Needle, Straight 1 1/2&#34;</td>
			<td style="width:147px;">Delasco Dermatology Lab &#38; Supply</td>
			<td style="width:77px;">KE-112</td>
			<td style="width:317px;"></td>
		</tr>
		<tr height="17">
			<td height="17" style="height:17px;width:347px;">Immunocal Decalcifying Solution</td>
			<td style="width:147px;">Fisher Scientific</td>
			<td>NC9491030</td>
			<td style="width:317px;"></td>
		</tr>
		<tr height="34">
			<td height="34" style="height:34px;width:347px;">Opticryl Acrylic Resin Bone Cement (PMMA) (Monomer and polymer)</td>
			<td style="width:147px;">US Dental Depot</td>
			<td style="width:77px;">OPTICRYL 100410&#160;</td>
			<td style="width:317px;"></td>
		</tr>
		<tr height="23">
			<td height="23" style="height:23px;width:347px;">Instron Model 5564 Tensile Testing Machine</td>
			<td style="width:147px;">Instron</td>
			<td style="width:77px;">5564</td>
			<td style="width:317px;">Any comparable tensile testing apparatus is suitable</td>
		</tr>
	</tbody>
</table>

athymic rat model for evaluation of engineered anterior cruciate ligament grafts

Anterior cruciate ligament (ACL) rupture is a common ligamentous injury that often requires surgery because the ACL does not heal well without intervention. Current treatment strategies include ligament reconstruction with either autograft or allograft, which each have their associated limitations. Thus, there is interest in designing a tissue-engineered graft for use in ACL reconstruction. We describe the fabrication of an electrospun polymer graft for use in ACL tissue engineering. This polycaprolactone graft is biocompatible, biodegradable, porous, and is comprised of aligned fibers. Because an animal model is necessary to evaluate such a graft, this paper describes an intra-articular athymic rat model of ACL reconstruction that can be used to evaluate engineered grafts, including those seeded with xenogeneic cells. Representative histology and biomechanical testing results at 16 weeks postoperatively are presented, with grafts tested immediately post-implantation and contralateral native ACLs serving as controls. The present study provides a reproducible animal model with which to evaluate tissue engineered ACL grafts, and demonstrates the potential of a regenerative medicine approach to treatment of ACL rupture.

하나의 외과 의사 92 쥐 수술의 우리의 경험에 의하면, 상처의 완료에 절개에서 수술 시간을 의미하는 것은 4.7 분, 표준 편차, 16.9 분이었다. 희생의 시점에서, 쥐 356 ± 23g의 무게. 모든 쥐들이 수술을 허용하고, 합병증을 경험하지. 즉시 수술 후, 쥐가 수술 말단에 무게를 견딜 언급,하지만 약간의 미약을 전시했다. 일주함으로써 수술 후 모든 쥐없이 감지 할 수있을 정도의 미약으로 ambulating했다. ?...

Watch this Scientific Journal Video about Athymic Rat Model for Evaluation of Engineered Anterior Cruciate Ligament Grafts at JoVE.com

Athymic Rat Model for Evaluation of Engineered Anterior Cruciate Ligament Grafts

Animal models are important tools for the evaluation of tissue-engineered grafts. This paper presents the protocol for preparing an electrospun biodegradable polymer graft for use in anterior cruciate ligament tissue engineering, as well as a surgical protocol for implantation in a rat model.

엔지니어링 전방 십자 인대 이식의 평가 흉선 쥐 모델

Anterior cruciate ligament (ACL) rupture is a common ligamentous injury that often requires surgery because the ACL does not heal well without ...

Research

JoVE Journal

Bioengineering

Magnetic Resonance Elastography Methodology for the Evaluation of Tissue Engineered Construct Growth

조직 평가에 대한 자기 공명 Elastography 방법론은 구조 성장하는 기술

Traditional mechanical testing often results in the destruction of the sample, and in the case of long term tissue engineered construct studies, the use ...

연구

생체공학

Surgical Retrieval, Isolation and In vitro Expansion of Human Anterior Cruciate Ligament-derived Cells for Tissue Engineering Applications

Surgical Retrieval, Isolation and In vitro Expansion of Human Anterior Cruciate Ligament-derived Cells for Tissue Engineering Applications

외과 검색, 분리 및<em&gt; 체외</em&gt; 조직 공학 응용 프로그램에 대한 인간의 전방 십자 인대에서 파생 된 세포의 확장

Injury to the ACL is a commonly encountered problem in active individuals. Even partial tears of this intra-articular knee ligament lead to biomechanical ...

Surgical Technique for the Implantation of Tissue Engineered Vascular Grafts and Subsequent In Vivo Monitoring

Surgical Technique for the Implantation of Tissue Engineered Vascular Grafts and Subsequent In Vivo Monitoring

티슈 엔지니어링 혈관 이식 및 이후의 이식을위한 수술 기법<em&gt; 생체</em&gt; 모니터링

The development of Tissue Engineered Vessels (TEVs) is advanced by the ability to routinely and effectively implant TEVs (4-5 mm in diameter) into a large ...

Generation and Grafting of Tissue-engineered Vessels in a Mouse Model

마우스 모델에서 조직 공학 혈관의 생성 및 손톱 확장

The construction of vascular conduits is a fundamental strategy for surgical repair of damaged and injured vessels resulting from cardiovascular diseases. ...

Production, Characterization and Potential Uses of a 3D Tissue-engineered Human Esophageal Mucosal Model

3 차원 조직 공학 인간의 식도 점막 모델의 제조, 특성 및 사용 가능성

The incidence of both esophageal adenocarcinoma and its precursor, Barrett&#8217;s Metaplasia, are rising rapidly in the western world. Furthermore ...

Engineered 3D Silk-collagen-based Model of Polarized Neural Tissue

편광 신경 조직의 엔지니어링 3D 실크 콜라겐 기반 모델

Despite huge efforts to decipher the anatomy, composition and function of the brain, it remains the least understood organ of the human body. To gain a ...

Applications of In Vivo Functional Testing of the Rat Tibialis Anterior for Evaluating Tissue Engineered Skeletal Muscle Repair

Applications of In Vivo Functional Testing of the Rat Tibialis Anterior for Evaluating Tissue Engineered Skeletal Muscle Repair

의 응용 프로그램<em&gt; 생체</em&gt; 조직 평가를위한 쥐 앞정 전방의 기능 테스트는 골격근 수리 엔지니어링

Despite the regenerative capacity of skeletal muscle, permanent functional and/or cosmetic deficits (e.g., volumetric muscle loss (VML) resulting from ...

Scaling of Engineered Vascular Grafts Using 3D Printed Guides and the Ring Stacking Method

3D 인쇄 가이드 및 반지 스태킹 방법을 사용하여 엔지니어링 혈관 이식의 크기 조정

Coronary artery disease remains a leading cause of death, affecting millions of Americans. With the lack of autologous vascular grafts available, ...

Treatment of Ligament Constructs with Exercise-conditioned Serum: A Translational Tissue Engineering Model

운동 조건의 혈청을 이용한 인대 구조의 치료 : Translational Tissue Engineering Model

In vitro experiments are essential to understand biological mechanisms; however, the gap between monolayer tissue culture and human physiology is large, ...

A New Ex Vivo Model for the Evaluation of Endoscopic Submucosal Injection Material Performance

A New Ex Vivo Model for the Evaluation of Endoscopic Submucosal Injection Material Performance

내 시경 Submucosal 주입 재료 성능 평가 대 한 새로운 Ex Vivo 모델

Increasing the performance of submucosal injection materials (SIMs) is important for endoscopic therapy of early gastrointestinal cancer. It is essential ...