Name: development of an algorithm to perform a comprehensive study of autonomic dysreflexia in animals with high spinal cord injury using a telemetry device
Uploaded: 2016-07-29T13:00:00
Description: Watch this Scientific Journal Video about Development of an Algorithm to Perform a Comprehensive Study of Autonomic Dysreflexia in Animals with High Spinal Cord Injury Using a Telemetry Device at JoVE

Denne forskningen hvis finansiert av Canadian Institute of Health Research and the Heart og stiftelsen av BC og Yukon Stroke. Vi ønsker å takke Mr. Rayshad Gopaul og Dr. Shelly McErlane for teknisk støtte og kompetanse i dyr omsorg.

Mannlige Wistar (Hsd: WI Wistar-rotter) ved 7 ukers alder og vekt på 300-350 g, ble anvendt i dette eksperimentet. Alle rottene ble holdt på en 12 timers lys / mørke syklus og fikk standard laboratorie-rottefor og vann ad libitum. Alle eksperimentelle prosedyrer dannet med guide til omsorg og bruk av forsøksdyr etablert av den kanadiske Council on Animal Care og gitt etikk godkjenning ved University of British Columbia. Kirurgi og dyr behandling ble utført i henhold til standard fremgangsmåter i vårt laboratorium...

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Den protokollen beskriver en detaljert implementering av en ny JAVA-plattformen AD Detection programvare som ville være kombinert med en telemetri-enhet, for en langvarig grundig analyse av ABP i SCI-dyr (figur 1B). Dette er den første programvaren som gjør det mulig for karakterisering av ABP-mønstre for å detektere spontane AD hendelser som de uregelmessige gjennom hele dagen. Et godt karakterisert T3 SCI dyremodell kan illustrere den funksjonelle kapasiteten til programvare for å oppdage frekve...

Autonom dysrefleksi (AD) er en livstruende krise i individer etter akutt eller kronisk ryggmargsskade (SCI) på livmorhalsen eller høy-thorax segmenter og er vanligvis preget av episoder av vedvarende hypertensjon og bradykardi en. AD er hovedsakelig forårsaket av forstyrrelser i synkende spinal veier som vanligvis gir innspill fra supraspinal sentrene til rygg sympatiske preganglionic nevroner som kontrollerer sympatisk aktivitet og vaskulær tone 1-4. AD episoder er preget av en topp i systolisk blodtrykk (SBP) opp til 300 mmHg og hvis den forblir ubehandlet kan føre til anfall, hemorrhagisk hjerneslag, hjerteinfarkt og død 5-8. En rekke giftige og ikke-giftige stimuli fungere som en utløser av AD, inkludert tarm og blære distensjon, spasmer, trykksår, urinblæren kateterisering eller iatrogen prosedyrer 9-12. Den tidsmessige utvikling av AD i hhvOnse til SCI har blitt undersøkt hos både mennesker 9 og dyremodeller 13,14. Vanligvis disse studiene har brukt en &quot;indusert AD &#39;metode (dvs. urodynamics, penile vibrostimulations hos mennesker eller kolorektal utspilt i dyr) for å bestemme tidsmessig utvikling av AD. En slik tilnærming er begrenset av behovet for gjentatte vurderinger ved isolerte tidspunkter som kan utelukke en nøyaktig bestemmelse av tidsmessige utviklingen av AD. Anvendelse av 24-timers overvåking av blodtrykket hos mennesker lar serieblodtrykksmålinger gjøres ved forhåndsbestemte intervaller. Denne teknikken har nylig blitt anvendt for å overvåke spontant forekommende AD hos pasienter med kronisk ryggmargsskade. I dyremodeller, er SSD-trykkgivere blir stadig mer brukt til kronisk overvåke slå-av-slå arterielle blodtrykket. Nylig, Rabchesvky et al. (2012), utviklet en algoritme som ekstraheres ett sekund gjennomsnitt av gjennomsnittlig arterietrykk (MAP) etd sammenlignet mot et glidende gjennomsnitt terskel 15. Spontane AD hendelsene ble preget basert på MAP topper som er 10 mmHg eller mer over terskel samtidig med en HR fall på 10 slag i minuttet eller mer. Her en roman JAVA programvare som har en innebygd AD algoritme er presentert. Denne algoritmen fungerer ved å registrere forhåndsbestemte mønstre i arterielt blodtrykk (ABP) og hjertefrekvens (HR) som er en indikasjon på et spontant forekommende AD hendelsen. Brukeren er i stand til å justere alle input variabler manuelt til programvaren slik at den &quot;algoritme&quot; kan enkelt tilpasses til de spesifikke parametre av interesse. Programvaren er også i stand til å dichotomize ABP og HR i en gitt epoke slik at dagaktive rhythmicity av hemodynamiske parametre kan analyseres 16. I den foreliggende manuskriptet, er en detaljert beskrivelse gitt av den kirurgiske teknikk som brukes for å implantere telemetri-enheter og utføre SCI kirurgi. examples er også utstyrt med hensyn til etterbehandlingsmulighetene i AD Detection programvare og hvordan hjerte-funksjon endres post-SCI. For sammenligningsformål, metodikk og resultater oppnådd fra en fremgangsmåte for indusert AD kjent som kolorektal utspilt (CRD) er også vist.

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development of an algorithm to perform a comprehensive study of autonomic dysreflexia in animals with high spinal cord injury using a telemetry device

Spinal cord injury (SCI) is a debilitating neurological condition characterized by somatic and autonomic dysfunctions. In particular, SCI above the mid-thoracic level can lead to a potentially life-threatening hypertensive condition called autonomic dysreflexia (AD) that is often triggered by noxious or non-noxious somatic or visceral stimuli below the level of injury. One of the most common triggers of AD is the distension of pelvic viscera, such as during bladder and bowel distension or evacuation. This protocol presents a novel pattern recognition algorithm developed for a JAVA platform software to study the fluctuations of cardiovascular parameters as well as the number, severity and duration of spontaneously occurring AD events. The software is able to apply a pattern recognition algorithm on hemodynamic data such as systolic blood pressure (SBP) and heart rate (HR) extracted from telemetry recordings of conscious and unrestrained animals before and after thoracic (T3) complete transection. With this software, hemodynamic parameters and episodes of AD are able to be detected and analyzed with minimal experimenter bias.

Ved hjelp av telemetri, er det arterielle blodtrykket samplet ved en frekvens på 1000 Hz kontinuerlig i 24 timer. En illustrerende opptak av arterielt blodtrykk (ABP) ved hjelp av LabChart er vist i figur 1B. Prøven ABP ble overvåket med en faststoff-trykksensor settes inn i den nedadgående aorta. Romanen JAVA-plattformen AD Detection programvare er i stand til å trekke ut relevante SBP (mmHg) topper (figur 1C). Vi kan også trekke HR (bpm) fra tidsintervallet mellom tilstøtende S...

Watch this Scientific Journal Video about Development of an Algorithm to Perform a Comprehensive Study of Autonomic Dysreflexia in Animals with High Spinal Cord Injury Using a Telemetry Device at JoVE

Development of an Algorithm to Perform a Comprehensive Study of Autonomic Dysreflexia in Animals with High Spinal Cord Injury Using a Telemetry Device

The catheter of a telemetry device is implanted into the abdominal aorta in order to continuously collect beat-by-beat hemodynamic data from animals pre and post-high thoracic spinal cord transection. A novel JAVA software was employed to analyze hemodynamic parameters as well as frequency and intensity of spontaneous episodes of autonomic dysreflexia.

Utvikling av en algoritme for å utføre en omfattende studie av Autonom dysrefleksi i dyr med høy ryggmargsskade Ved hjelp av en Telemetri Device

Spinal cord injury (SCI) is a debilitating neurological condition characterized by somatic and autonomic dysfunctions. In particular, SCI above the ...

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A Murine Model of Cervical Spinal Cord Injury to Study Post-lesional Respiratory Neuroplasticity

En Murint Model of Cervical Spinal Cord Injury å studere Post-lesional Respiratory Neuroplasticity

A cervical spinal cord injury induces permanent paralysis, and often leads to respiratory distress. To date, no efficient therapeutics have been developed ...

Controlled Cortical Impact Model for Traumatic Brain Injury

Kontrollert Cortical Impact Modell for traumatisk hjerneskade

Every year over a million Americans suffer a traumatic brain injury (TBI).  Combined with the incidence of TBIs worldwide, the physical, emotional, ...

A Radio-telemetric System to Monitor Cardiovascular Function in Rats with Spinal Cord Transection and Embryonic Neural Stem Cell Grafts

En Radio-telemetrisk system for å overvåke kardiovaskulær funksjon i rotter med ryggmargstran og Embryonic Neural Stem Cell Grafts

High thoracic or cervical spinal cord injury (SCI) can lead to cardiovascular dysfunction. To monitor cardiovascular parameters, we implanted a catheter ...

Synergetic Use of Neural Precursor Cells and Self-assembling Peptides in Experimental Cervical Spinal Cord Injury

Synergisk Bruk av Neural forløperceller og Selv montering Peptider i Experimental Cervical ryggmargsskade

Spinal cord injuries (SCI) cause serious neurological impairment and psychological, economic, and social consequences for patients and their families. ...

Calibrated Forceps Model of Spinal Cord Compression Injury

Calibrated pins Modell av ryggmargskompresjon Injury

Compression injuries of the murine spinal cord are valuable animal models for the study of spinal cord injury (SCI) and spinal regenerative therapy. The ...

Contrast Enhanced Ultrasound Imaging for Assessment of Spinal Cord Blood Flow in Experimental Spinal Cord Injury

Kontrastforsterket ultralyd Imaging for Assessment of Spinal Cord Blood Flow i Experimental ryggmargsskade

Reduced spinal cord blood flow (SCBF) (i.e., ischemia) plays a key role in traumatic spinal cord injury (SCI) pathophysiology and is accordingly an ...