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DOI :
10.3791/52839-v
•
10:27 min
November 10th, 2015
Chapters
0:05
Title
1:58
Template Preparation
3:46
PCR Amplification
5:37
HRM Analysis
8:26
Results: Blocked-probe-based and MAAB Increases HRM Genotyping Sensitivity
9:46
Conclusion
高解像度融解分析は、不均一な集団中の単一塩基多型を区別する能力を提供するが、変異対立遺伝子の増幅偏りは、試料内の比較的低い割合で存在する対立遺伝子を検出する能力を高めることができます。このプロトコルは、高分解能融解分析の感度を向上させる改良が記載されています。
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