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DOI :
10.3791/52851-v
•
11:44 min
May 13th, 2015
Chapters
0:05
Title
1:49
Isolation of Individual Transposon Mutants
3:44
Evaluation of Bacterial Concentration and Single Primer Colony PCR
5:34
Eukaryotic Cell Challenge with Coxiella Mutants and Monitoring of Intracellular Growth
7:17
Preparation of Samples for Automated Image Acquisition
9:25
Results: Viability and Infectivity of Coxiella Transposon Mutants
11:14
Conclusion
Coxiella burnetii의는 인수 공통 질병 Q 열병에 대한 책임 의무적 세포 내 그람 음성 세균이다. 여기에서 우리는 Coxiella 형광 트랜스포존 돌연변이의 발생뿐만 아니라 자동 식별 및 결과 국제화, 복제 및 세포 독성 표현형의 분석 방법을 설명합니다.
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