May 25th, 2015
•Designer chromosomes of the Synthetic Yeast Genome project, Sc2.0, can be distinguished from their native counterparts using a PCR-based genotyping assay called PCRTagging, which has a presence/absence endpoint. Here we describe a high-throughput real time PCR detection method for PCRTag genotyping.
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved