Name: a mouse model of subchronic and mild social defeat stress for understanding stress-induced behavioral and physiological deficits
Uploaded: 2015-11-24T14:00:00
Description: Watch this Scientific Journal Video about A Mouse Model of Subchronic and Mild Social Defeat Stress for Understanding Stress-induced Behavioral and Physiological Deficits at JoVE.com

作者感谢博士。长冈健太郎（东京农工大学）和饭尾涉（茨城县）的有益的讨论。这项研究是由农业和医学科学（IUCAM）（文部科学省，日本）和研究项目上的农产品和健康促进的好处食品的发展（NARO）之间的茨城大学合作的部分资助（农林水产省，日本） 。

动物研究，批准并满足两个动物护理和茨城大学使用委员会和教育，文化，体育，科学部，技术部（MEXT），日本（71号通知）的准则。该协议的一个完整的概述如图 1所示。 1.仪器<ol><li>配制2种笼：单笼（宽（W）×深度研究[D]×高[H] =143毫米×293毫米×148毫米），和“社会溃败（SD）”笼（W×D× H = 220毫米×320毫米×135毫米）。 </li><li> 如图2，划分的SD笼...

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有sCSDS小鼠和CSDS小鼠进行标准CSDS协议19（与每天侵略者物理接触5至10分钟）之间的差异确定的体重。时的应力期间sCSDS小鼠显示增加BWG，而标准CSDS小鼠显示体重降低时的应力期间21,22,23。有这两种协议之间的侵略者与身体接触的总时长方面在10天的期限压力很大差异。而原来的CSDS范例包括侵略者和从属小鼠中总（全面CSDS）之间的物理相互作用的50或100分钟时，sCSDS方法包含这种相?...

多种压力事件发生在整个人类的生活。过大的应力常导致在人类和动物有害的生理后果。在人类中，压力事件是主要危险因素沉淀精神障碍，例如抑郁症1。疾病研究的全球负担表明，抑郁症是最致残性疾病的伤残调整寿命年（DALY）和多年居住残疾2项之一。此外，抑郁症占自杀的DALY 3的比例最大。患有抑郁症很难管理自己的生活，作为一个结果，他们的生活质量往往恶化。因此，强烈需要开发有效的治疗剂，以改善生活在这些患者中的质量。 许多研究的重大抑郁症被执行，并揭示了疾病susceptibilit的遗传贡献y为约30〜40％，这是由多个位点的小的影响4的作用进行说明。由于抑郁症背后的复杂的致病机理，疾病的病因的详细仍不清楚。临床报告显示，有抑郁症，亚型如忧郁和非典型抑郁症5，其显示降低的和分别6增加体重。虽然25-30％和抑郁症患者15-30％为纯忧郁和非典型特征，分别大多有两种亚型7的混合特征。因此，严重抑郁症有广泛的症状。为了找到生物标志物和开发目标的治疗剂的各类人类的抑郁症的，它建立凹陷8的几个不同的动物模型是重要的。 抑郁症的动物模型用几种方法已经建立了一个包括学无奈，慢性应激和慢性社会的失败应力（CSDS）9-12。丰田和同事建立大鼠和小鼠13-17的CSDS模型，以阐明代谢和与抑郁症相关的行为。由于抑郁症的动物模型是由表面效度18评估，在其中模型建立的环境是很重要的。此外，金等 19报道的方法进行详细创建CSDS小鼠。已知的是，在CSDS小鼠的社会行为的缺陷可以通过慢性治疗中回收，而不是由急性治疗，与抗抑郁药，而且它们在脑源性​​神经营养的调节方面共享的症状类似于在抑郁症患者因素6。 Goto等13以前开发的亚慢性和轻度社会败应力（sCSDS）小鼠模型通过修改方法金等人 。19。该sCSDS小鼠显示以下收益体重和增加身体水分含量13 polydipsia-和亢进样症状。在这份报告中，提供了协议建立sCSDS小鼠模型和我们讨论这个模型的效用。

<table border="0" cellpadding="0" cellspacing="0">
	<tbody>
		<tr>
			<td>single cage</td>
			<td>Charles River Laboratories Japan</td>
			<td></td>
			<td>width [W] &#215; depth [D] &#215; height [H] = 143 &#215; 293 &#215; 148 mm</td>
		</tr>
		<tr>
			<td>M cage</td>
			<td>Natsume Seisakusho</td>
			<td></td>
			<td>W &#215; D &#215; H = 220 &#215; 320 &#215; 135 mm</td>
		</tr>
		<tr>
			<td>Whiteflake</td>
			<td>Charles River Laboratories Japan</td>
			<td></td>
			<td>Wood-shaving chips made from spruce trees</td>
		</tr>
		<tr>
			<td>AIN-93G</td>
			<td>Oriental Yeast</td>
			<td></td>
			<td>purified-diet food pellets</td>
		</tr>
		<tr>
			<td>Kimtowel</td>
			<td>Nippon Paper Crecia Co.</td>
			<td></td>
			<td>Paper towels</td>
		</tr>
		<tr>
			<td>open-field arena</td>
			<td>O&#8217;Hara &#38; Co.</td>
			<td></td>
			<td>made of gray polyvinylchloride</td>
		</tr>
	</tbody>
</table>

a mouse model of subchronic and mild social defeat stress for understanding stress-induced behavioral and physiological deficits

Stressful life events often increase the incidence of depression in humans. To study the mechanisms of depression, the development of animal models of depression is essential. Because there are several types of depression, various animal models are needed for a deeper understanding of the disorder. Previously, a mouse model of subchronic and mild social defeat stress (sCSDS) using a modified chronic social defeat stress (CSDS) paradigm was established. In the paradigm, to reduce physical injuries from aggressors, the duration of physical contact between the aggressor and a subordinate was reduced compared to in the original CSDS paradigm. sCSDS mice showed increased body weight gain, food intake, and water intake during the stress period, and their social behaviors were suppressed after the stress period. In terms of the face validity of the stress-induced overeating and overdrinking following the increased body weight gain, the sCSDS mice may show some features related to atypical depression in humans. Thus, a mouse model of sCSDS may be useful for studying the pathogenic mechanisms underlying depression. This protocol will help establish the sCSDS mouse model, especially for studying the mechanisms underlying stress-induced weight gain and polydipsia- and hyperphagia-like symptoms.

以监测物理应力的程度超过10天的期间，攻击叮咬ICR小鼠的数目由研究人员进行手动计数。 图5A指示各值接收攻击叮咬的数目。有在早期阶段（第1天约10-120叮咬）相当大的变化，但这种变化被减少了后期（第10天约为：5-20叮咬）。 图5B表示接收攻击叮咬的平均数量逐渐降低随着时间的推移，由于物理接触的持续时间减少（从5分钟至0.5分钟）。 受试者...

Watch this Scientific Journal Video about A Mouse Model of Subchronic and Mild Social Defeat Stress for Understanding Stress-induced Behavioral and Physiological Deficits at JoVE.com

A Mouse Model of Subchronic and Mild Social Defeat Stress for Understanding Stress-induced Behavioral and Physiological Deficits

这里，为了开发亚慢性和轻度社会败应力的小鼠模型中的方法描述和用于研究抑郁症的致病特性，包括hyperphagia-和烦渴样症状以下增加体重。

亚慢性和温和的社会失败应激的小鼠模型理解应激诱导行为和生理赤字

Stressful life events often increase the incidence of depression in humans. To study the mechanisms of depression, the development of animal models of ...

Research

JoVE Journal

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The Resident-intruder Paradigm: A Standardized Test for Aggression, Violence and Social Stress

驻地入侵者范式：一种标准化考试的侵略，暴力和社会压力

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哺乳期鼠害使用慢性社会压力型号产后抑郁症

Exposure to chronic stress is a reliable predictor of depressive disorders, and social stress is a common ethologically relevant stressor in both animals ...

Moderate Prenatal Alcohol Exposure and Quantification of Social Behavior in Adult Rats

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Alterations in social behavior are among the major negative consequences observed in children with Fetal Alcohol Spectrum Disorders (FASDs). Several ...

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颅脑损伤后大鼠行为缺陷的检测

With the increasing incidence of traumatic brain injury (TBI) in both civilian and military populations, TBI is now considered a chronic disease; however, ...

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诱导小鼠快感缺失的不可预测慢性轻度应力协议

Depression is a highly prevalent and debilitating condition, only partially addressed by current pharmacotherapies. The lack of response to treatment by ...

Stress-Enhanced Fear Learning, a Robust Rodent Model of Post-Traumatic Stress Disorder

压力增强恐惧学习--创伤后应激障碍的健壮啮齿动物模型

Fear behaviors are important for survival, but disproportionately high levels of fear can increase the vulnerability for developing psychiatric disorders ...

A Visual Guide for Studying Behavioral Defenses to Pathogen Attacks in Leaf-Cutting Ants

叶切蚁病原体攻击行为防御研究的可视化指南

The complex lifestyle, evolutionary history of advanced cooperation, and disease defenses of leaf-cutting ants are well studied. Although numerous studies ...