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DOI :
10.3791/52982-v
August 27th, 2015
Chapters
0:05
Title
1:30
Isolation of Primary Mouse Hepatocytes
5:27
Fatty Acid Oxidation Assay
8:55
Lipogenesis Assay
10:13
Results: Fatty Acid Oxidation and Lipogenic Responses to Respiration Altering Compounds
11:33
Conclusion
从头脂肪合成和β-脂肪酸氧化构成肝关键代谢途径,被扰动在几种代谢疾病,包括脂肪肝疾病途径。在这里,我们证明了小鼠原代肝细胞的分离和描述了β-脂肪酸氧化和脂肪生成量化。
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