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DOI :
10.3791/52982-v
August 27th, 2015
Chapters
0:05
Title
1:30
Isolation of Primary Mouse Hepatocytes
5:27
Fatty Acid Oxidation Assay
8:55
Lipogenesis Assay
10:13
Results: Fatty Acid Oxidation and Lipogenic Responses to Respiration Altering Compounds
11:33
Conclusion
デノボ脂質生成およびβ脂肪酸酸化は、肝細胞に脂肪肝疾患を含むいくつかの代謝障害に摂動された経路を主要な代謝経路を構成しています。ここでは、マウス初代肝細胞の単離を実証し、β - 脂肪酸の酸化および脂質生成の定量化について説明します。
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