Name: a 'plug and play' method to create water-dispersible nanoassemblies containing an amphiphilic polymer, organic dyes and upconverting nanoparticles
Uploaded: 2015-11-14T13:00:00
Description: Watch this Scientific Journal Video about A 'Plug and Play' Method to Create Water-dispersible Nanoassemblies Containing an Amphiphilic Polymer, Organic Dyes and Upconverting Nanoparticles at JoVE.com

This research was supported by the Natural Sciences and Engineering Research Council (NSERC) of Canada, the Canada Research Chairs Program, and Simon Fraser University. This work made use of 4D LABS shared facilities supported by the Canada Foundation for Innovation (CFI), British Columbia Knowledge Development Fund (BCKDF) and Simon Fraser University.

NaYF 4 / Ybの3+ / ER 3+アップコンバーティングナノ粒子（UCNP）の1合成<ol><li>以下のように装置を設定します。 <ol><li>定期的な撹拌プレート上で250ミリリットル加熱マントルを置き、熱電対の上にマントを差し込みます。 </li><li>適切なクランプで加熱マントル上に磁気撹拌棒を備えた250mlの丸底フラスコを置きます。 </li><li>丸底フラスコの左首に空気アダプタを取り付?...

<ol>
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このプロトコルに従って合成されたナノ粒子は、約22.5 nmの中心20から25ナノメートルのサイズ分布を有する。26,27それらはα-NaYF 4ホスト格子構造を有する球状粒子として分類することができます。このプロトコルで2つの重要なステップがあります。 UCNP合成では、粒子サイズの狭い分布を確実にするために、可能な限り正確な加熱温度および時間を維持することが重要です。?...

今日バイオイメージング剤の新しいタイプを開発する緊急の必要性が依然として存在します。多くの新規蛍光プローブが十分に文書化されている。1-6ただし 、画像の解像度の大幅な改善が課題である。7つの実用的な方法は、直接「光」の発光状態と「暗い」急冷状態との間の蛍光プローブを変調することです。 8-12この特定の方法は、誘導放出の枯渇（STED）顕微鏡13及び確率的光学再構築顕微鏡（STORM）などの技術を開発するために適用されている。14 蛍光を調節するための別のアプローチは、蛍光プローブと一緒に夫婦光応答性発色団である。15,16は、異性体の一方のみが、効率的なエネルギー移動アクセプターとして機能することができる2つの異性体間光応答発色団の切り替え、目からの蛍光の消光の制御を可能にしますフェルスター共鳴エネルギー移動（FRET）、および他の機構を介して電子プローブ。結果は、発光状態と異なる波長の光に光応答性発色団の暴露によって交互に行うことができる急冷状態の作成です。 光応答ジアリールエテン発色団は、可逆的に無色の開環異性体およびUVおよび可視光の照射により着色された閉環体との間で切り替えることができます。17〜19の熱2つの異性体の安定性と閉環体メイクの調整可能な吸収スペクトルジアリールエテン非常に良好な候補を制御FRETアクセプターとして。20-23ランタニドドープNaYF 4アップコンバージョンナノ粒子は、バイオイメージングのために有用である。24これらのナノ粒子は、近赤外光を吸収し、可視スペクトルのいくつかの領域の光を放出します。光応答ジアリールエテン発色団とナノ粒子を組み合わせることで蛍光変調の例としては、事前にされていますviously我々のグループによって報告された。25-27しかしながら 、各実施例に記載されたシステムは、より多様なシステムの開発を複雑にナノ粒子の表面にジアリールエテンを取り付けるために追加の合成修飾を必要としました。 本明細書において、我々は、自己組織化戦略を使用して水分散性有機色素分子と光応答アップコンバートナノ粒子を調製するための単純な「プラグ・アンド・プレイ」方式を示します。ポリマーの選択。 2070アミンポリ（スチレン- ALT -無水マレイン酸）とポリエーテルは、疎水性および親水性環境の両方を提供します。ポリマーの親水性領域は、水溶解性を維持するために重要であるのに対し、ポリマーヘルプの疎水性セクションでは、一緒に通常は水に不溶性の有機分子とアップコンバートナノ粒子を保持します。私たちは、第1の熱核法でアップコンバートナノ粒子の合成を実証します。そこで、ホを証明します有機分子とアップコンバートナノ粒子wのポリマーシェルの疎水性領域内にカプセル化されており、単純に便利なワークアップ手順に続いてアップコンバートナノ粒子、ポリマーと異なる有機色素分子の溶液を、共同攪拌して水性媒体中で安定したまま。また、外光照射を使用してアセンブリの蛍光発光を変調する方法を示します。私たちは、水分散性nanoassembliesが拡大していきますするには、この「プラグアンドプレイ」メソッドを使用しての範囲を期待しています。

<table border="0" cellpadding="0" cellspacing="0">
	<tbody>
		<tr>
			<td>yttrium acetate</td>
			<td>sigma</td>
			<td>326046</td>
			<td>Yttrium(III) acetate hydrate</td>
		</tr>
		<tr>
			<td>ytterbium acetate</td>
			<td>sigma</td>
			<td>544973</td>
			<td>&#160;Ytterbium(III) acetate hydrate&#160;</td>
		</tr>
		<tr>
			<td>erbium acetate</td>
			<td>sigma</td>
			<td>325570</td>
			<td>Erbium(III) acetate hydrate</td>
		</tr>
		<tr>
			<td>oleic acid</td>
			<td>sigma</td>
			<td>75096</td>
			<td>analytical standard</td>
		</tr>
		<tr>
			<td>octadecene</td>
			<td>sigma</td>
			<td>O806&#160;</td>
			<td>Technical grade&#160;</td>
		</tr>
		<tr>
			<td>NaOH</td>
			<td></td>
			<td>S5881&#160;</td>
			<td>reagent grade</td>
		</tr>
		<tr>
			<td>NH4F</td>
			<td></td>
			<td>216011</td>
			<td>ACS reagent</td>
		</tr>
		<tr>
			<td>poly(styrene-alt-maleic anhydride)</td>
			<td>sigma</td>
			<td>4422699</td>
			<td>Average Mn= 1700</td>
		</tr>
		<tr>
			<td>JeffAmine 2070</td>
			<td>Huntsman</td>
			<td>M-2070</td>
			<td></td>
		</tr>
		<tr>
			<td>Varian Carry 300</td>
			<td>Agilent</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>JDSU NIR laser</td>
			<td>JSDU</td>
			<td>L4-9897510-100M</td>
			<td>980 nm diode laser</td>
		</tr>
	</tbody>
</table>

a 'plug and play' method to create water-dispersible nanoassemblies containing an amphiphilic polymer, organic dyes and upconverting nanoparticles

In this protocol, we first describe a procedure to synthesize lanthanide doped upconverting nanoparticles (UCNPs). We then demonstrate how to generate amphiphilic polymers in situ, and describe a protocol to encapsulate the prepared UCNPs and different organic dye molecules (porphyrins and diarylethenes) using polymer shells to form stable water-dispersible nanoassemblies. The nanoassembly samples containing both the UCNPs and the diarylethene organic dyes have interesting photochemical and photophysical properties. Upon 365 nm UV irradiation, the diarylethene group undergoes a visual color change. When the samples are irradiated with visible light of another specific wavelength, the color fades and the samples return to the initial colorless state. The samples also emit visible light from the UCNPs upon irradiation with 980 nm near-infrared light. The emission intensity of the samples can be tuned through alternate irradiation with UV and visible light. Modulation of fluorescence can be performed for many cycles without observable degradation of the samples. This versatile encapsulation procedure allows for the transfer of hydrophobic molecules and nanoparticles from an organic solvent to an aqueous medium. The polymer helps to maintain a lipid-like microenvironment for the organic molecules to aid in preservation of their photochemical behavior in water. Thus this method is ideal to prepare water-dispersible photoresponsive systems. The use of near-infrared light to activate upconverting nanoparticles allows for lower energy light to be used to activate photoreactions instead of more harmful ultraviolet light.

吸収スペクトルおよび発光スペクトルは、サンプルDAE-UCNPのために収集しました。吸収スペクトルは、閉じたジアリールエテン発色団とアップコンバートナノ粒子間のスペクトルの重複を比較するために使用されています。サンプル（TPP-UCNP両方DAE-UCNP）の写真は、水性相中の両親媒性ポリマーシェル内に配置された有機色素分子およびアップコンバートナノ粒子の成功したカプセル化を実証?...

Watch this Scientific Journal Video about A 'Plug and Play' Method to Create Water-dispersible Nanoassemblies Containing an Amphiphilic Polymer, Organic Dyes and Upconverting Nanoparticles at JoVE.com

A 'Plug and Play' Method to Create Water-dispersible Nanoassemblies Containing an Amphiphilic Polymer, Organic Dyes and Upconverting Nanoparticles

Organic dye molecules and oleic acid coated upconverting nanoparticles are not water-soluble. This protocol describes a &#8216;plug and play&#8217; method that enables the transfer of organic dye molecules and upconverting particles from their initial hydrophobic solvent to water.

「プラグアンドプレイ」両親媒性ポリマー、有機染料及びアップコンバーティングナノ粒子を含む水分散性Nanoassembliesを作成する方法を

In this protocol, we first describe a procedure to synthesize lanthanide doped upconverting nanoparticles (UCNPs). We then demonstrate how to generate ...

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Chemistry

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We demonstrate a straightforward protocol to graft pristine multiwalled carbon nanotubes (MWCNTs) with polystyrene (PS) chains at the sidewalls through a ...

Electrochemical Impedance Spectroscopy as a Tool for Electrochemical Rate Constant Estimation

電気化学インピー ダンス分光電気化学定数推定法のためのツールとして

Electrochemical impedance spectroscopy (EIS) was used for advanced characterization of organic electroactive compounds along with cyclic voltammetry (CV). ...

Time-resolved Photophysical Characterization of Triplet-harvesting Organic Compounds at an Oxygen-free Environment Using an iCCD Camera

時間分解光物性評価のトリプレット収穫有機化合物無酸素環境使用、iCCD カメラ

Here, we present a sensible method of the acquisition and analysis of time-resolved photoluminescence using an ultrafast iCCD camera. This system enables ...

Raman and IR Spectroelectrochemical Methods as Tools to Analyze Conjugated Organic Compounds

ラマン散乱とツールとしての IR 分光電気化学法共役有機化合物を分析するには

In the presented work, two spectroelectrochemical techniques are discussed as tools for the analysis of the structural changes occurring in the molecule ...

Controlled Photoredox Ring-Opening Polymerization of O-Carboxyanhydrides Mediated by Ni/Zn Complexes

Controlled Photoredox Ring-Opening Polymerization of O-Carboxyanhydrides Mediated by Ni/Zn Complexes

OCarboxyanhydrides ニッケル/亜鉛錯体による媒介のユウロピウムの開環重合

Here, we describe an effective protocol that combines photoredox Ni/Ir catalysis with the use of a Zn-alkoxide for efficient ring-opening polymerization, ...