Photopatterning proteínas y células en el medio ambiente acuoso Usando TiO
October 26th, 2015
•Hideaki Yamamoto1,2, Takanori Demura3, Kohei Sekine3, Sho Kono3, Michio Niwano2,4, Ayumi Hirano-Iwata2,5, Takashi Tanii6
1Frontier Research Institute for Interdisciplinary Sciences, Tohoku University, 2CREST, Japan Science and Technology Agency, 3School of Fundamental Science and Engineering, Waseda University, 4Research Institute of Electrical Communication, Tohoku University, 5Graduate School of Biomedical Engineering, Tohoku University, 6Faculty of Science and Engineering, Waseda University
We describe a protocol for modifying cell affinity of a scaffold surface in aqueous environment. The method takes advantage of titanium dioxide photocatalysis to decompose organic film in the photo-irradiated region. We show that it can be used to create microdomains of scaffolding proteins, both ex situ and in situ.
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