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El estudio de la función de proteínas y el Papel de la expresión de la proteína alterada por la interferencia de anticuerpos y reconstrucciones tridimensionales

DOI :

10.3791/53049-v

April 21st, 2016

April 21st, 2016

6,555 Views

1Institute for Biochemistry, Emil-Fischer Centre, University of Erlangen-Nuremberg, 2Department of Biology, Animal Physiology, University of Erlangen-Nuremberg

Controlling protein expression is not only essential to every organism alive, but also an important strategy to investigate protein functions in cellular models. The protocol presented shows the application of antibody interference in mammalian cells including primary hippocampal neurons and demonstrates the use of three-dimensional reconstructions in studying protein function.

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